Characterization of Embryonic Stages through Variations in the Egg＇s Contents in Spodoptera fitura

This paper describes the embryonic development of the common cutworm Spodoptera litura （Fabricius） （Lepidoptera： Noctuidae） which is divided into eight embryonic stages, through variations in the egg＇s contents. Experiments were conducted on the eggs ofS. litura under laboratory conditions. The highest concentration of the protein （min.-max.： 32-! 40 lag/egg） and the lowest of the carbohydrate （min.-max.： 3.0-44.0 lag/egg） were obtained; similarly, the DNA content was smaller （min.-max.： 1.0-38.2 lag/egg） than the RNA （min.-max.： 9.2-360.0 lag/egg） throughout the embryogenesis. Three peaks of ecdysteroid titre were observed which were responsible for appearance of particular events during embryogenesis. The last highest peak of 400_＋33.4 lag/egg was appeared at 84 h after oviposition when larva was ready to hatch. Therefore, it is concluded that eight embryonic stages in S. litura were characterized by fluctuation in the egg＇s contents, which were responsible for gradual-progressive embryonic development. The egg-larval development did not show a normal distribution of embryonic developmental-time.     

Effect of NaCl-induced saline sodicity on the interpretation of soil potassium dynamics

Excess of exchangeable sodium (Na) in salt-affected soils causes ion toxicity and decrease in nutrient uptake by plants, particularly potassium (K). A number of studies have been conducted to investigate the effect of K-fertilization on plant growth under sodic and saline-sodic conditions but the results are much diverse to process for concrete recommendations. To explore the possible reasons, it was hypothesized that Na applied as NaCl to produce salinity/sodicity in the soil may release non-exchangeable K, minimizing the effect of K-fertilization. Incubation studies were conducted for 2, 4 and 6 days in the light (sandy loam) and heavy (clay loam) textured soils producing two saline/sodic levels, i.e. 20 and 30 sodium adsorption ratio (SAR) along with control (SAR 3). Potassium fertilizer applied was calculated according to 40 (general recommendations based on soil-nutrient status), 80 and 160 kg K ha^?1. Interestingly, it was observed that addition of NaCl possibly released non-exchangeable K from the soil minerals and increased the K concentration in soil solution. Total K release was more in heavy textured soil but initial release was more in light textured soil. This release may eliminate the effect of K-fertilization applied under salt stress induced by NaCl. Therefore, it is suggested that while studying Na–K interaction in salt-affected soils, NaCl should be avoided to produce salinity, and naturally occurring saline-sodic soils may be used. Soil Na–K interaction studies including ameliorating effect of K under sodic or saline-sodic conditions should be conducted carefully considering the above-stated argument.     

Seed Priming Influence on Early Crop Growth,Phenological Development and Yield Performance of Linola (Linum usitatissimum L.)

Reduced early crop growth and limited branching are amongst yield limiting factors of linola. Field response of seed priming treatments viz. 50 mmol L⁻¹ salicylic acid (SA), 2.2% CaCl₂ and 3.3% moringa leaf extract (MLE) including untreated dry and hydropriming controls was evaluated on early crop growth and yield performance of linola. Osmopriming with CaCl₂ reduced emergence time and produced the highest seedling fresh and dry weights including Chl. a contents. Osmopriming with CaCl₂ reduced crop branching and flowering and maturity times and had the maximum plant height, number of branches, tillers, pods and seeds per pod followed by MLE. Increase in seed weight, biological and seed yields was 9.30, 34.16 and 39.49%, harvest index (4.12%) and oil contents (13.39%) for CaCl₂ osmopriming. Positive relationship between emergence and seedling vigor traits, 100-seed weight, seed yield with maturity time, 100-seed weight and seed yield were found. The study concludes that seed osmopriming with CaCl₂ or MLE can play significant role to improve early crop growth and seed yields of linola.     

Habitat use and preliminary demographic evaluation of the critically endangered Mediterranean monk seal (Monachus monachus) in the Cilician Basin (Eastern Mediterranean)

Over the last few decades a dramatic decline in the number of Mediterranean monk seals (Monachus monachus) limited scientific studies on the biology, behaviour, and ecology of the species. This lack of information impaired the effectiveness of conservation strategies. Thus, any further information gathered on the monk seal has utmost value in the work to halt and reverse the plight of the species. A hitherto unknown Cilician Basin colony of the Mediterranean monk seal has been investigated during a 6-year period between 1995 and 2001. Results of direct in-cave surveys and land based seal-watch observations enabled the evaluation of habitat use and preliminary demographic information. A total of 39 caves were discovered among which only three were used for breeding. Scarcity of the breeding caves emphasised the importance of breeding habitat in the survival of the colony. Common features of the breeding caves are found as an entrance with a protective barrier against strong waves; a chamber with a wide beach; and a well-sheltered shallow pool. Supplementary data obtained from infrared monitors installed in three actively used caves were used to assess the number of individuals. Throughout the study period, 25 individuals were identified and 11 newborn pups were found. Evaluation of the results suggests the presence of sub-regions inhabited by an individually identified sub-group of monk seals. Recommendations for conservation guided by this information is a Marine Protected Area consisting of two zones; a restricted core zone prohibiting any human activity securing breeding habitat and a restricted fishery zone securing sustainability of the food source.     

A reliable gender diagnostic PCR assay for jojoba (<Emphasis Type="Italic">Simmondsia chinensis</Emphasis> (Link) Schneider)

Jojoba (Simmondsia chinensis (Link) Schneider) is an obligate cross-pollinated shrub native to the Sonora desert. The most valuable product of the jojoba seeds is the liquid wax or jojoba oil which is marketed extensively in the cosmetic industry. Differing from the most of the cultivated crop species, jojoba has slow-growing habit, difficulties in the determination of sex at the early seedling stages, a male-biased ratio and low seed yield. In jojoba, the development of molecular strategies for the identification of sexes at early stages has been a priority in plantations and breeding programs. Two previous studies reported two candidate male-specific jojoba DNA markers. However, present study indicated that these markers were not useful in jojoba sex identification. A reliable gender diagnostic marker for jojoba is, therefore, needed. In the present study a novel jojoba male-specific touch-down polymerase chain reaction based DNA marker (JMS900) was reported using a total of 120 individual jojoba plants bulked into 16 samples. This sex specific DNA marker may have considerable theoretical and practical applications in the establishment of jojoba plantation and breeding studies.     

Genetic diversity of Russian common wheat varieties for leaf rust resistance

The results of evaluating resistance to leaf rust pathogen in Russian common wheat varieties recommended for growing in the Russian Federation are given. Their diversity with respect to Lr genes is assessed with the use of phytopathological and molecular methods and representativeness in growing regions is analyzed. A low genetic diversity among resistant varieties with domination of genes Lr9 and Lr19 and their confinement to Volga, Ural, and West Siberian regions are revealed.     

Development and utilization of diagnostic DAMD-PCR markers for <Emphasis Type="Italic">Capsicum</Emphasis> accessions

A polymerase chain reaction (PCR) based approach involving the directed amplification of minisatellite DNA region (DAMD-PCR) was used to identify accession specific DNA markers and study genetic relationships between and within 15 accessions corresponding to 11 species in genus Capsicum. A touch down PCR profile and unique chemical concentration of ingredients resulted in reproducible and reliable DNA amplifications. The number of amplified products varied from 1 to 12 fragments depending on the template DNA and the primers. The DAMD-PCR technique provided a total of 38 accession specific DNA markers (diagnostic DAMD-PCR) which can be utilized in accession identification, preservation and genetic studies of Capsicum germplasm. Based on 1,292 polymorphic and monomorphic DNA markers directed with 22 minisatellite specific primers, accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Capsicum chacoense was found to be the most distinct species.     

Evaluation of the effects of topical tripeptide-copper complex and zinc oxide on open-wound healing in rabbits

In this study the clinical and histopathological effects of topically applied tripeptide-copper complex (TCC) and zinc oxide on open-wound healing in rabbits was evaluated. Eighteen New Zealand rabbits were divided into three groups: TCC, zinc oxide and no treatment. One full thickness wound was created on each side of the dorsal midline in each rabbit. Wound margins were traced on days 0, 7, 14, and 21 and topical TCC and zinc oxide were applied daily for 21 days to each rabbit in the respective treatment groups. The mean unhealed wound area was significantly smaller in the TCC than in the zinc oxide group on day 7, but it was significantly smaller in the TCC group than in to the control group on days 7, 14 and 21. The mean percentage of wound contraction on day 7 was significantly higher in the TCC than in the zinc oxide group; however, it was significantly higher in the TCC group than in the control group on days 7, 14 and 21. Median time for the coverage of the wound bed with granulation tissue was significantly shorter in the TCC group than in the other groups. Filling of the open wound with granulation tissue to skin level was significantly slower in the control group than in the other two groups. Neutrophil counts decreased regardless of the group in parallel with healing, while neovascularization was best observed in the TCC group. The results suggest that TCC is a better choice in the treatment protocols of open wounds in rabbits than zinc oxide.     

Early detection of the effects of compaction in forested soils: evidence from selective extraction techniques

Purpose

Soil compaction resulting from mechanisation of forest operations reduces air permeability and hydraulic conductivity of soil and can result in the development of hydromorphic and/or anoxic conditions. These hydromorphic conditions can affect physico-chemical properties of the soils. However, early detection of these effects on mineralogical portion of soils is methodologically difficult.

Materials and methods

To analyse the effects of soil compaction on iron minerals in loamy Luvisol, three compacted and three non-compacted soil profiles up to the depth of 50 cm were collected from an artificially deforested and compacted soils after 2 years of treatment. Soil was compacted with the help of 25 Mg wheeler’s load to increase the dry bulk density of soil from 1.21?±?0.05 to 1.45?±?0.1 g cm^?3. Soil samples were analysed by X-ray diffraction (XRD) and were treated by citrate bicarbonate (CB) and dithionite citrate bicarbonate (DCB) under controlled conditions. Major and minor elements (Fe, Al, Mg, Si and Mn) were analysed by ICP-AES in the CB and DCB extracts.

Results and discussion

It was found that X-ray diffraction is not an enough sensitive method to detect the quick mineralogical changes due to soil compaction. Results obtained from CB-DCB extractions showed that soil compaction resulted in larger CB and smaller DCB extractable elements as compared to non-compacted soil. Labile Fe was found 30 % of total Fe oxides in compacted soil against 10–14 % in non-compacted soils. Compaction thus resulted in Fe transfer from non-labile to labile oxides (s.l.). Results showed that soil compaction leads to the reduction of Fe³⁺ to Fe²⁺. The effects of hydromorphic conditions due to soil compaction were observed up to the depth of 35 cm in forest soil profile. Furthermore, a close association of Al with Fe oxides was observed in the soil samples, while Mn and Si were mainly released from other sources, Mg showing an intermediate behaviour.

Conclusions

Hydromorphic conditions owing to soil compaction affect the mobility and crystallisation process of iron mineral. CB-DCB selective extraction technique, in contrast to XRD technique, can be effectively used to examine the possible effects of soil compaction on iron minerals.

     

The conjunctive use of compost tea and inorganic fertiliser on the growth,yield and terpenoid content of Centella asiatica (L.) urban

The conjunctive use of a compost tea (CT) and an inorganic fertiliser (NPK) on the growth, yield and terpenoid content of Centella asiatica (L.) urban was evaluated. CT and NPK applied at half the recommended concentration resulted in the significant enhancement of vegetative growth, yield and antioxidant content. The synergistic effect of CT₅₀ and NPK₅₀ was highly pronounced on the bioactive components Asiatic acid, madecassoside and asiaticoside. The distribution of the total antioxidants was highest in roots (80%), followed by leaves (66%) and petioles (54%). There was a strong positive correlation (r = 0.990) between fertility and total antioxidant content, suggesting an enhanced synthesis of bioactive components resulting from the treatment. This response by C. asiatica to the integrated experimental treatment suggests a viable option for the commercial cultivation of the herb. Augmenting the vegetative biomass production and bioactive components will increase the availability of this medicinal herb for the treatment of various human ailments.