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Current study was conducted to identify constituents of Taxus wallichiana Zucc. that might be responsible for its folk use in anti-inflammatory conditions. Taxusabietane A was isolated from the bark extract of Taxus wallichiana Zucc. Taxusabietane A was analyzed for in-vitro and in-vivo anti-inflammatory activities using Lipoxygenase (LOX) inhibition assay and carrageenan-induced paw edema model. Taxusabietane A revealed considerable LOX inhibitory activity with the IC50 value being 57 ± 0.31. Standard compound Baicalein showed the IC50 value being 22.1 ± 0.03 μM. Taxusabietane A also showed significant (5 and 10 mg/kg) anti-inflammatory activity induced by carrageenan. However, this study highlighted the potential of Taxusabietane A to be further explored as a new lead compound for management of conditions associated with inflammation.  相似文献   
33.
Extensive subsurface drainage system was installed in districtMardan in the North West Frontier Provinceof Pakistan in 1987 to control increasingwater logging and salinity problems due tocanal irrigation. Several recentlycompleted fields studies have indicatedthat subsurface drainage system hasenormously lowered watertable in certainareas due to extensive drainage network. Therefore, a study of controlled subsurfacedrainage technique was initiated in MardanScarp area to observe the temporal andspatial variations in water table depths ofthis specific case under various modes ofcanal irrigation and monsoon rains. Twoartificially drained areas, consisting of40 ha and 160 ha respectively, werecontrolled and selected for extensivemonitoring. A total of 98 observationswells (7.6 cm dia. and 4.1 m depth) wereinstalled in between lateral drains toobserve water table fluctuation. Theresults of this study are very interesting.Each of the two areas monitored in thestudy behaved differently. It was observedthat in one of the areas design water tabledepth at 1.1 m was maintained with properfunctioning of the controlled techniqueapplied to the subsurface drainage system. The results from this area showed that 25to 55% of the time throughout the yearachieved this objective whereas in thesecond area desired water table could notbe maintained and water table depth in thisarea remained between 2.0 to 2.7 m causingunnecessary water stress to plants. Alsoit was observed that watertable in theformer area is mostly controlled by thefunctional behavior of the irrigationcanal. In addition, the proper functioningof controlled techniques in subsurfacedrainage system supplemented veryefficiently to retain the groundwater levelto the optimal limits in dry season and tothe design ones in the others for timelyneeds of the crops. Also rainfalls havesignificant impact on the spatial andtemporal behaviors of water table depths inboth the areas during the monsoon season.  相似文献   
34.
In this study, we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed by SDS-PAGE. Fourteen bands ranging from 25 to 240 kDa in size were observed for cats, and 17 bands ranging from 25 to 289 kDa were observed for dogs. The densities of the bands differed among control, diabetic, and insulin-treated animals. In conclusion, we found that serum protein profiles and IGF-I concentrations were altered in both diabetic and insulin-treated animals. When judiciously interpreted in the light of other clinical and laboratory data, the techniques used in our study provide a valuable modality for measuring the severity of diabetes mellitus in dogs and cats.  相似文献   
35.
The composite effects of gum arabic (GA) (5, 10, 15, and 20%) and chitosan (CH) (1.0%) on the biochemical and physiological characteristics of banana fruits stored at 13 ± 1 °C and 80 ± 3% relative humidity (RH) for 28 days and afterward for 5 days at simulated marketing conditions (25 °C, 60% RH) were investigated. Significant (P ≤ 0.05) differences were observed for the entire GA plus CH treatments as compared to the control. However, the results showed that after 33 days of storage, the weight loss and soluble solids concentration of fruits treated with 10% GA plus 1.0% CH composite coating were 24 and 54% lower, whereas fruit firmness, total carbohydrates, and reducing sugars were 31, 59, and 40% higher than the control, respectively. Furthermore, the composite edible coating of 10% GA plus 1.0% CH delayed color development and reduced the rate of respiration and ethylene evolution during storage as compared to the control. Similarly, sensory evaluation results also proved the effectiveness of 10% GA plus 1.0% CH composite coating by maintaining the overall quality of banana fruits. Consequently, the results of scanning electron microscopy also confirmed that the fruits coated with 10% GA plus 1.0% CH composite edible coating had very fewer cracks and showed a smooth surface. These findings suggest that 10% GA plus 1.0% CH as an edible composite coating can be used commercially for extending the storage life of banana fruits for up to 33 days.  相似文献   
36.
严格控制由刺盘孢属(Colletotrichum spp.)真菌引起的炭疽病对减少热带水果工业的经济损失十分重要。本文主要研究了阿拉伯胶(GA)(10%)、柠檬香草精油(LG)(0.05%)、肉桂精油(CM)(0.4%)及其复合组分在试验条件下对离体与活体香蕉和番木瓜炭疽病菌的抑制活性。结果表明,0.05%的LG和0.4%的CM对于引起香蕉和番木瓜炭疽病的两种病原菌Colletotrichum musae和Colletotrichum gloeosporioides分别具有抑制活性;单独GA处理并未表现出抑菌活性,但将0.05%LG、0.4%CM与10%GA混合处理则具有较大的抑菌活性;在各处理中,以PDA培养基中加入10%GA及0.4%CM的处理对C.musae和C.gloeosporioides这两种病原菌抑制活性最显著,对于两种病原菌菌丝生长抑制率分别为73.4%和70.0%,对孢子萌发抑制率分别为88%和85%。活体试验结果也表明,10%GA结合0.4%的CM处理具有最佳的作用效果,对C.musae和C.gloeosporioides的抑制方面表现出较强的增效作用,对人工接种的香蕉和番木瓜炭疽病导致的腐烂抑制率分别为80%和71%。品质评价的试验结果也表明,10%GA结合0.4%CM的涂膜处理具有良好效果,果实失重率、硬度、可溶性固形物和可滴定酸含量的变化均表明该处理可显著推迟果实的成熟。上述结果表明,10%阿拉伯胶结合0.4%肉桂精油处理可以作为控制诸如香蕉和番木瓜等常见热带水果采后炭疽病的生物杀菌剂。  相似文献   
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ABSTRACT

A pot study was conducted on Gujranwala series (Udic Haplustalf) to compare zinc (Zn) requirement of maize hybrids (FHY- 456, FHY- 396, and FHY- 421) and indigenous varieties (EV-1089, Golden, and Soneri). Uniform rates of nitrogen:phosphorus:potassium (N:P:K) and four rates of Zn were applied in triplicate according to CRD. There was a significant (P < 0.05) main and interactive effect of maize genotypes and zinc application on shoot growth and Zn uptake. The three maize varieties uniformly produced maximum shoot dry weight at 3 mg Zn kg?1 soil. The maize hybrids produced maximum shoot dry weight at 9 mg Zn applied kg?1 soil. A 6.3 mg Zn kg?1 plant tissue was optimum for FHY-421 (hybrid) and 9.5 mg Zn kg?1 plant tissue was optimum for Soneri (variety). Hence, more pronounced response in maize hybrids than indigenous varieties require higher rates of Zn application. However, further verification of the results is warranted under field conditions.  相似文献   
38.
ABSTRACT

Microbial fortified organic amendment in chili cultivation may affect plant development and disease suppression. Microbial infused rice straw compost, commercial rice straw compost, and fungicide Benomyl for chili (Capsicum annum L.) cultivation and control of Sclerotium foot rot were studied under glass house condition. Chili seed cv. Kulai were sown in the Sclerotium rolfsii infested and non-infested soil. After two weeks, five healthy seedlings were transplanted into planting bags. Growth performance and development of disease symptoms associated with S. rolfsii foot rot infection were assessed. Applying microbial infused rice straw compost increased seed germination and plant growth, and suppressed development of foot rot compared to using commercial rice straw compost and the Benomyl. A higher disease reduction (84.6%) occurred with 15 Mg · ha?1 microbial infused rice straw compost (62.7%), followed by Benomyl (53.8%), and 15 Mg · ha?1 commercial rice straw compost (46.2%). Application of microbial infused rice straw compost at 15 Mg · ha?1 yielded optimum seed germination and seedling establishment, plant growth, and disease suppression. Microbial infused rice straw compost is a good alternative to chemical fungicide in controlling Sclerotial disease in chili.  相似文献   
39.
In this study, we report on pyrazin-2(1H)-ones as lead for the development of potent adenosine triphosphate (ATP) competitive protein kinase inhibitors with implications as anti-cancer drugs. Initially, we identified the pyrazin-2(1H)-one scaffold from hamacanthins (deep sea marine sponge alkaloids) by Molecular Modeling studies as core binding motif in the ATP pocket of receptor tyrosine kinases (RTK), which are validated drug targets for the treatment of various neoplastic diseases. Structure-based design studies on a human RTK member PDGFR (platelet-derived growth factor receptor) suggested a straight forward lead optimization strategy. Accordingly, we focused on a Medicinal Chemistry project to develop pyrazin-2(1H)-ones as optimized PDGFR binders. In order to reveal Structure-Activity-Relationships (SAR), we established a flexible synthetic route via microwave mediated ring closure to asymmetric 3,5-substituted pyrazin-2(1H)-ones and produced a set of novel compounds. Herein, we identified highly potent PDGFR binders with IC50 values in an enzymatic assay below µM range, and possessing significant activity against PDGFR dependent cancer cells. Thus, marine hamacanthin-derived pyrazin-2(1H)-ones showing interesting properties as lead for their further development towards potent PDGFR-inhibitors.  相似文献   
40.
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