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通过反转录-聚合酶链反应(RT-PCR),从中国北京分离株牛流行热病毒JB76H基因组RBA中扩增出主要保护性抗原糖蛋白G的cDNA。采用Sanger’s双脱氧末端终 止法测定cDNA片段的核苷酸序列,并推导出氨基酸序列。G基因全长为1872个碱基,单一的开放阅读框架阅读框架编码623个氨基酸的多肽。将测得的序列与澳大利亚六个分离株进行比较,发现我国分离株与渊大利亚分离株间同源性为91%,低于澳大利亚各分 相似文献
124.
印度的农业生产条件比我国要恶劣的多,但也正是因为印度积极加强农业基础设施建设,才在农业的很多方面超越了中国。印度农业设施建设的基本经验,对于我国加快社会主义新农村建设,促进农业向集约化现代化转变提供了极其重要的借鉴和启示。 相似文献
125.
淋雨和添加剂对苜蓿青贮品质的影响 总被引:1,自引:0,他引:1
为研究淋雨和添加剂对苜蓿(Medicago sativa)青贮品质的影响,试验设新鲜和淋雨苜蓿青贮2个因素,添加剂分别为对照、甲酸、绿汁发酵液、改良绿汁发酵液、玉米粉、绿汁发酵液+玉米粉、改良绿汁发酵液+玉米粉7个水平,共14个处理。青贮90 d后,测定各处理的pH、氨态氮、有机酸、营养成分和硝基化合物含量。结果表明:淋雨和添加剂均极显著降低苜蓿青贮饲料pH、氨态氮、乳酸和乙酸含量(P<0.01)。改良绿汁发酵液+玉米粉组的乳酸含量显著高于对照和其他处理组(P<0.05)。淋雨和添加剂显著影响苜蓿青贮饲料的干物质、酸性洗涤纤维、中性洗涤纤维含量和缓冲能值(P<0.05),添加剂对粗灰分、粗蛋白、粗脂肪和水溶性碳水化合物含量有显著影响(P<0.05)。淋雨、添加剂及其交互作用极显著影响苜蓿青贮饲料的硝酸盐和亚硝酸盐含量(P<0.01)。添加剂可改善淋雨苜蓿青贮的发酵品质,其中改良绿汁发酵液+玉米粉组合效果最明显。 相似文献
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YU Lei HAN Bing TIAN Tian ZHENG Lu YANG Ting LIU Xing TANG Lei LUO Xuan YANG Qin XIE Ru-jia 《园艺学报》2017,33(12):2151-2156
AIM: To investigate the effect of suberoylanilide hydroxamic acid (SAHA) on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and to explore its possible mechanism. METHODS: HepG2 cells were treated with SAHA at different concentrations for 48 h. The proliferation of HepG2 cells was detected by real-time cellular analysis. The protein levels of acetylated histones H3K9 and H3K27, glucose-regulated protein 78 (GRP78), protein kinase R-like endoplasmic reticulum kinase (PERK) and p-PERK were determined by Western blot. The cell apoptosis was analyzed by flow cytometry. RESULTS: Compared with control group, treatment with SAHA at 0.1 μmol/L and 1 μmol/L for 48 h showed no significant inhibitory effect on the proliferation of HepG2 cells, while SAHA at 6 μmol/L and 12 μmol/L significantly inhibited the proliferation of HepG2 cells (P<0.05). The results of Western blot showed that the protein levels of acH3K9, acH3K27, GRP78 and p-PERK increased significantly after treated with SAHA at diffe-rent concentrations for 48 h, while the protein level of PERK was decreased significantly (P<0.05). The results of flow cytometry analysis showed that the apoptotic rates of the HepG2 cells increased with the increase in SAHA concentration. CONCLUSION: SAHA up-regulates the acetylation of H3K9 and H3K27 in the HepG2 cells and induces apoptosis of HepG2 cells by activating the endoplasmic reticulum stress-related apoptosis pathway. 相似文献
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128.
LI Jin-bo XIA Ming-yuan WAN Bing-liang DU Xue-shu ZHA Zhong-ping YU Da-zhao QI Hua-xiong 《水稻科学》2009,16(1):79-82
A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene. 相似文献
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AIM: To investigate the effect of SIRT1 on the autophagy of pancreatic cancer cells under hypoxia condition, and to analyze the underlying mechanism of regulating FOXO1/RAB7 signaling pathway. METHODS: Western blot and immunofluorescence methods were used to determine the expression of SIRT1 in the pancreatic cancer cells. The small interfering RNA targeting SIRT1 and SIRT1 over-expression plasmid were transfected into the pancreatic cancer Panc-1 cells. Confocal microscopy was used to detect the LC3 expression. Western blot was used to analyze the protein levels of LC3, p62 and FOXO1/RAB7 signaling pathway-related molecules. Co-immunoprecipitation was used to detected the protein interaction between SIRT1 and FOXO1. RESULTS: The expression level of SIRT1 in the nucleus of Panc-1 cells was increased under hypoxia condition. Compared with negative control under hypoxia condition, knock-down of SIRT1 expression attenuated the autophagy flux in the pancreatic cancer Panc-1 cells (P<0.05). Over-expression of SIRT1 increased the protein levels of FOXO1 and RAB7. On the contrary, knock-down of SIRT1 expression inhibited the protein levels of FOXO1 and RAB7. The protein interaction between SIRT1 and FOXO1 in the pancreatic cancer cells was observed. CONCLUSION: SIRT1 in pancreatic cancer Panc-1 cells under hypoxia condition is over-expressed in the nucleus. Down-regulation of SIRT1 inhibits autophagy and its mechanism may be related to FOXO1/RAB7 signaling pathway. 相似文献