回归分析法在累积流量测量中的应用

在非定点使用标准表的标准装置中,进行累积流量的测量时要用到流量计的仪表系数。通过实例计算介绍了另一种求得仪表系数的方法,即回归分析法。利用这种方法求得的流量计的仪表系数,由于减少了人为的误差,因此,大大提高了计算整个累积流量测量过程总液量的准确度,同时提高了工作效率。     

STAT6介导的巨噬细胞极化对布鲁氏菌胞内存活的影响

旨在探讨STAT6介导的巨噬细胞极化对布鲁氏菌胞内生存的影响。本研究采用布鲁氏菌光滑株S2308（S2308）和粗糙型疫苗株RB51（RB51）侵染巨噬细胞。利用qRT-PCR检测M1型巨噬细胞标志因子p65、NOS2和IL-1β,M2型巨噬细胞标志因子STAT6、ARG1、IL-10的mRNA表达水平;流式细胞术检测M1型标记分子CD86和M2型标记分子CD206的表达;Western blot检测p-STAT6蛋白及抑制剂AS对蛋白的抑制作用;ELISA检测M1型细胞因子TNF-α、IL-12和M2型细胞因子IL-4、IL-10的表达量;最后对胞内菌落进行CFU计数。qRT-PCR结果显示,在感染8、12 h时可显著诱导M1型因子mRNA转录表达,72 h时低表达,而M2型因子在72 h时高表达;流式细胞术结果显示,S2308感染12 h可显著诱导CD86的表达,感染72 h可显著诱导CD206的表达,但RB51对二者无影响;Western blot结果显示,S2308菌株在感染72 h时激活STAT6信号通路,而RB51几乎不激活该通路,抑制剂AS在2 μmol·L^-1浓度时抑制效果最佳;ELISA结果显示,AS抑制剂可显著抑制IL-4、IL-10的释放,并促进TNF-α、IL-12的释放;CFU计数结果显示,S2308组的胞内菌呈先降低后显著上升趋势,加入AS抑制剂后可显著抑制布鲁氏菌胞内复制。布鲁氏菌S2308在感染后期能够通过STAT6诱导M1型巨噬细胞向M2型转化,并促进Th2型细胞因子的释放,从而有利于布鲁氏菌的胞内生存。而RB51几乎不激活该通路,不影响胞内生存。     

The Effect of Training on Yili Horse 1 600 m Test Results and Heart Rate Variability

【Objective】 The objective of this study was to analyze the effect of different training stages on the performance and heart rate variability(HRV) of Yili horses and to provide data for reference in the conditioning training of 1 600 m distance Yili horses.【Method】 Eight 3-year-old Yili horses (stallions) were selected as test subjects.A 3-month speed specific performance training program was conducted, and a speed test race was organized in the last week of each month of training, and HRV was collected before, immediately after, 0.5 h after, and 1 h after 1 600 m test race.Among them, the time-domain indexes included the mean of all R-R intervals (Mean RR), the standard deviation of all R-R intervals (SDNN), the mean heart rate (Mean HR), the root mean square of the difference between adjacent R-R intervals (RMSSD), the number of adjacent R-R intervals with a difference greater than 50 ms (NN50), and the percentage of adjacent R-R intervals with a difference greater than 50 ms to the total number of heartbeats (pNN50).Frequency domain metrics included very low frequency (VLF), low frequency power (LF), high frequency power (HF) and nonlinear metrics:standard deviation (Y) of all R-R-spacing (SD1), standard deviation (X) of all R-R-spacing (SD2).Consequently, the variability of HRV indexes in horses at different training stages was analyzed.【Result】 The race time in the post training period in 1 600 m speed test race of Yili horses was significantly lower than that in the early training period (P<0.05), and Mean RR, NN50 and pNN50 in the middle and post training periods were significantly lower than that in the early training period (P<0.05).Mean HR in the beginning of training was significantly lower than that in post training (P<0.05).VLF and LF in the end of training were significantly lower than that in the beginning of training (P<0.05).【Conclusion】 Under the conditions of this test, the HRV indexes of 1 600 m test race of Yili horses in different conditioning training stages were analyzed.The outcomes demonstrated that the types of neural activity in Yili horses at the beginning, middle and end of training presented some differences, showing changes of increased sympathetic excitability and decreased parasympathetic excitability, and the horses' athletic performance improved.Therefore, HRV could be considered as an effective tool to evaluate the training load and intensity of Yili horses during training.     

蜀宣花牛体型外貌、线性评定及生理指标测定

[目的]为了充分了解牛群的体型外貌及生理指标情况,我们对牛群体型外貌进行了评定、同时对牛群的毛色、肤色、踢、角及生理指标进行了调查研究。[方法]通过对牛群生理指标测定、体型外貌评定及毛色、肤色、踢、角颜色和形状分析。[结果]表明:蜀宣花牛肉用指数公母牛分别为5.3769和4.0725,低于国外专门化肉牛品种,但也明显高于国内几个比较有名的地方牛品种,由此说明,蜀宣花牛的肉用指数界于专用型肉牛和肉役兼用型牛之间。毛色以黄白花和红白花为主,占90.16%;皮肤颜色以纯粉色和粉色有斑点的为主,占96.92%;蹄质颜色以纯蜡黄色为主,占86.28%;角型以照阳角为主,占93.94%。成年母牛的生理指标分别为:体温平均38.38℃、心跳74.56次/min、呼吸18.74次/min和瘤胃蠕动2.84次/2min;体型外貌线性评定分析表明,蜀宣花牛母牛的14个性状中,除后房宽度、尻角度和乳房底部3个性状平均分略低于75分,强壮度、后房高度、乳头位置和乳静脉4个性状的平均分高于80分外,其余7个性状的平均分都在75~80分之间。[结论]从乳用性能的角度看,牛群的平均体型尚处在较理想型和理想型之间,还有待进一步选育提高。     

蚕丝纤维在反相乳液中对酸性染料的吸附性能

为了达到节水节能的目的,使用非离子型表面活性剂TX-100、正辛醇、异辛烷和酸性染料水溶液制备用于蚕丝纤维染色的非离子型反胶束体系。首先研究pH值和增溶水量对染料平衡吸附量的影响,接着使用酸性橙156、酸性黑234和酸性媒介黑9对蚕丝纤维进行吸附实验,并使用Langmuir和Freundlich等温吸附模型对实验数据进行拟合,考察染料结构与染料平衡吸附量之间的关系。结果表明,在反胶束体系中pH值的增加不利于蚕丝对酸性染料的吸附,在增溶水量W=10时染料的平衡吸附量达到极大值。此外,蚕丝纤维对酸性染料的吸附模型符合Langmuir吸附模型,该吸附过程为单分子吸附,并属于优惠吸附;蚕丝对酸性媒介黑9的平衡吸附量最大,这与染料的分子结构有关。     

柑橘新品种‘华红橘 1 号’

 ‘华红橘1 号’为浙江地方红橘品种‘满头红’的芽变,具有丰产性好、品质优良、风味浓 郁、化渣性好、酸甜适度等优良性状。在湖北荆门果实成熟期为11 月中下旬。     

卵黄抗体及其在家禽生产中的应用

1 IgY的性质及特点卵黄抗体(IgY)是禽类B淋巴细胞在抗原物质刺激下所产生,是母鸡将血液中的特异性抗体选择性地(主要是IgG)转移并沉积到卵黄,能与抗原发生特异性反应的一种球蛋白,是禽类在长期进化过程中形成的用于提高雏禽抵抗力的物质[1].     

青藏高原东南部冻土区夏季降水及河水氢氧同位素的变化特征

基于青藏高原2019年7月31日至2019年8月10日采集的5个降水样品、25个河水样品、1个湖水样品,结合气象资料,进行青藏高原东南部冻土区冻融期夏季降水和河水同位素方法与海拔、降水量、气温相关关系分析,同时采用克里金插值分析了δ18O和δD的空间分布特征。结果表明,夏季河水水量受气温影响显著,冻土融化水与河水交互作用更加密切,表明土壤冻融过程在多年冻土区径流过程中起到重要影响。