马尾松银松素合酶基因启动子区的克隆及特征分析

银松素合酶(pinosylvin synthase,PS)是合成银松素类植物抗毒素的关键酶。本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。结果表明,在翻译起始密码子上游167 nt处可能存在TATA-box。此外还发现3个GATA-box(-260 nt、-295 nt、-386 nt和-295 nt)和若干个TGAC-like序列。     

三种禾草萌发期抗旱性研究

采用蒸馏水及浓度为5%、10%、15%、20%和25%的PEG溶液处理梭罗草、青海草地早熟禾、垂穗披碱草种子,测定其发芽率、苗长、胚根/胚芽比值,以研究PEG对种子萌发的影响。结果显示:PEG浓度为5%时,青海草地早熟禾的相对发芽率出现小幅上升趋势,垂穗披碱草和梭罗草均呈下降趋势;不同处理对胚根/胚芽比值和苗长均有影响,梭罗草受影响小。因此这三种牧草在萌发期抗旱性依次为:梭罗草、青海草地早熟禾、垂穗披碱草。     

卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇、磷脂含量的影响

为了探索绵羊冷冻精液受精率低的原因,在稀释液中用低密度脂蛋白代替卵黄冷冻绵羊精液,研究LDL对冷冻中精子脂类含量的影响。结果表明,以卵黄稀释液冷冻精液,精子胆固醇含量从鲜精的234．54nmol／10^9降低到163．76nmol／10^9精子,胆固醇与磷脂的比值（c／p）从0．54下降到0．36;以9g／100mL LDL代替卵黄,冷冻后胆固醇含量为243．73nmol／10^9精子,c／p值保持在0．50。研究得出在卵黄稀释液中冷冻绵羊精液,精子胆固醇流失,c／p值下降;以LDL代替卵黄,可有效保护精子胆固醇,避免流失,维持了精子c／p值。     

不同施用方式下保水剂浓度对作物成苗的影响

针对内蒙古干旱地区农业生产实际和保水剂在节水农业中的应用前景,对干旱条件下保水剂浓度对作物出苗和生长的影响进行了研究。通过盆栽试验,研究了保水剂不同施用方式下,浓度对萝卜、玉米和糜子的出苗率和幼苗生长的影响。结果表明：施用保水剂可显著提高作物出苗率并改善幼苗生长状况,但其效果因施用方式不同,差异较大。对萝卜而言,5cm施用深度下,沟施时对应浓度在0．10％～0．50％范围内,出苗率比对照提高25．3～44．0个百分点,混施在浓度小于1．00％时,皆能提高出苗率,但提高幅度明显低于沟施;10cm施用深度下,混施时浓度在0．10％～0．50％范围内,出苗率比对照高30个百分点左右,而沟施则不明显。对于玉米和糜子,混施浓度低于0．30％时,玉米出苗率提高5～7倍,糜子出苗率提高2～4倍。从幼苗生长看,萝卜在试验最低浓度0．10％,玉米和糜子在0．10％～0．30％,各处理株高明显优于对照。总体上保水剂和土壤混施优于沟施,施用深度对作物成苗的影响在不同施用方式下不一致,这种差异与保水剂和土壤的相互作用有关。     

玉米叶片保绿度F1杂种优势与环境互作效应分析

采用加性-显性及与环境互作的遗传模型,利用玉米6个亲本,按完全双列杂交设计组配的15个杂交组合在4种环境条件下的试验资料,分析玉米叶片保绿度F1杂种优势与环境互作效应。结果表明:玉米叶片保绿度F1杂种优势存在着极显著的基因型与环境互作效应。不同杂交组合在同一环境下的F1杂种优势表现不尽相同,同一杂交组合在不同环境条件下表现也不尽相同。玉米自交系沈137是选育保绿性玉米杂交种较好的亲本材料。     

不同发育时期玉米叶片保绿度遗传主效应及其与环境互作效应分析

采用包括基因型×环境互作效应的加性-显性遗传模型及非条件和条件的分析方法,研究多环境下玉米吐丝后不同发育时期叶片保绿度性状的遗传主效应及其与环境互作效应。结果表明:非条件遗传分析表明控制玉米叶片保绿度在发育不同时期的各遗传效应均受到环境条件的影响并存在着表达水平上的差异;条件遗传方差分量分析表明,控制玉米吐丝后不同时期叶片保绿度表现的净遗传效应在各个发育时期均有新的不同程度的表达,对玉米由抽丝期到成熟期发育进程基因表达净效应的选择越来越受到环境条件的影响。     

The peroxisomal matrix shuttling receptor Pex5 plays a role in FB1 production and virulence in Fusarium verticillioides

The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B₁(FB₁) production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression o...     

紫椴组培快繁技术研究

为探索建立紫椴组织培养快速繁育体系,以紫椴种子为材料,探讨基本培养基、植物生长调节剂浓度及配比对紫椴组培快繁效果的影响。结果表明,增殖培养基以1/2MS培养基最适宜,添加0.05 mg/L 6-BA和0.03 mg/L IBA后,增殖系数可达13.5;MS培养基最适宜做壮苗培养基和生根培养基,最佳壮苗培养基为MS+0.1 mg/L6-BA+0.1 mg/L IBA+0.03 mg/LGA₃,平均苗高达5.15 cm;最佳生根培养基为MS+1.0 mg/L6-BA+0.05 mg/L NAA,生根率达93.3%,生根数达5.7根。     

Inducible nitric oxide synthase and arterial pressure regulation

AIM and METHODS:To clarify the role of inducible nitric oxide synthase (iNOS) in the regulation of blood pressure,in the present study, we examined the effect of aminoguanidine (AG), a selective inhibitor of iNOS on the hemodinamical response of Dahl salt-sensitive (DS) and Dahl salt-resistant (DR) rats to low (0.3%) or high (8%) sodium chloride (NaCl) infusion by chronical in vivo hemodynamic experiment, and the effect of NaCl or NaCl plus AG infusion on urinary nitrate (NO₃)/nitrite (NO₂), the end product of nitric oxide (NO), excretion by Greiss Reaction. Furthermore, NOS activity assay was also carried out to probe the effect of NaCl and AG on calcium-dependent or independent NOS activity in renal tissue. RESULTS:1. High or low NaCl-infused DR rats and low NaCl-infused DS rats have no hemodinamical response to AG, however, the hypertensive effect of high NaCl (8%) infusion on DS rats were greatly amplified by co-infusion of AG. 2. Administration of high NaCl significantly elevated the iNOS activity of renal tissue, and greatly increased urinary NO₃/NO₂ excretion. CONCLUSION:Inducible NOS is an important modulator of arterial pressure, especially in case of higher blood pressure.     

Effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits

AIM:To investigate the effect of dimethyl sulfoxide on endotoxin -induced fever in rabbits. METHODS:Test substances were administered intravenously into a marginal ear vein. Temperature responses were measured using rectal thermistor probes. RESULTS:Endotoxin (0.4 μg/kg) stimulated a biphasic rise in colonic temperature. Pretreatment with dimethyl sulfoxide significantly attenuated the biphasic febrile response to endotoxin (0.4 μg/kg) of conscious rabbits in a dose-dependent manner. Rabbits treated with dimethyl sulfoxide 10 min after injection of endotoxin developed fever, which was significantly milder compared with rabbits treated with an equal volume of normal saline. No significant differences were found between thermal response index of rabbits injected with dimethyl sulfoxide and those with normal saline. CONCLUSION:Dimethyl sulfoxide inhibit endotoxin-induced fever in rabbits.