四倍体鸭茅产量及其构成因素的QTL定位

产量相关性状是复杂的数量性状,对鸭茅的单株产量及构成因素进行QTL分析,可提高育种中对产量性状优良基因选择的效率,同时为鸭茅遗传改良、基因克隆及分子标记辅助育种提供理论依据。以四倍体鸭茅“楷模”和“01436”为亲本杂交而成的作图群体为试验材料,于2014年对洪雅、宝兴两个不同生境下鸭茅株高、旗叶长、倒二叶长、旗叶宽、倒二叶宽、茎粗、花序长、分蘖数、单株干重等9个产量相关性状进行了表型鉴定及相关性分析。此外,在已构建的高密度鸭茅分子遗传图谱的基础上,采用MapQTL 5.0进一步对这些性状QTL定位分析。结果表明,绝大多数性状在亲本间呈显著差异且都整体表现出连续变异,符合数量性状遗传的特征;相关性分析表明,大多数产量相关性状均与干重极显著正相关,与单株干重相关性最好的依次为分蘖、株高;QTL分析发现,控制该9个农艺性状的QTL共60个,洪雅38个,宝兴22个,这些QTL分别定位于分子连锁图谱的1、2、3、4、5共5个连锁群上,单个QTL的贡献率为5.7%~24.7%,单个性状QTL个数为2~15个。其中,控制株高、花序长的QTL各12个,控制倒二叶长、茎粗的QTL各4个,控制旗叶宽、倒二叶宽的QTL各2个,控制单株干重的QTL有6个,影响分蘖的QTL为3个,与旗叶长相关的QTL最多15个。     

Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene

The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB₁₂, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor.     

Multimarker and rare variants genomewide association studies for bone weight in Simmental cattle

Bone weight, defined as the total weight of the bones in all the forequarter and hindquarter joints, can reflect somebody conformation traits and skeletal diseases. To gain a better understanding of the genetic determinants of bone weight, we used a composite strategy including multimarker and rare‐marker association to perform genomewide association studies (GWAS) for that character in Simmental cattle. Our strategy consisted of three models: (i) A traditional linear mixed model (LMM) was applied (Q+K‐LMM); (ii) single nucleotide polymorphisms (SNPs) with p‐values less than .05 from the LMM were selected to undergo the least absolute shrinkage and selector operator (Lasso) in the second stage (LMM‐Lasso); (iii) genes containing two or more rare SNPs were examined by performing the sequence kernel association test (gene‐based SKAT). A total of 1,225 cattle were genotyped with an Illumina BovineHD BeadChip containing 770,000 SNPs. After the quality‐control procedures, 1,217 individuals with 608,696 common SNPs and 105,787 rare SNPs (with 0.001 < minor allele frequency [MAF] <0.05) remained in the sample for analysis. A traditional LMM successfully mapped three genes associated with bone weight, while LMM‐Lasso identified nine genes, which included all genes found by traditional LMM. Only a single gene, EPHB3, surpassed the significance threshold after Bonferroni correction in gene‐based SKAT. In conclusion, based on functional annotation and results from previous endeavours, we believe that LCORL, RIMS2, LAP3, PRKAR2B, CHSY1, MAP2K6 and EPHB3 are candidate genes for bone weight. In general, such a comprehensive strategy for GWAS may be useful for researchers seeking to probe the full genetic architecture underlying economic traits in livestock.     

乙烯利对干旱胁迫下草地早熟禾生理指标的影响

以草地早熟禾(Poa pratensis)‘Nuglade'品种为试验材料,研究叶片喷施乙烯利对草地早熟禾在水分充足、干旱及复水恢复3种处理下叶片电解质渗透率、叶绿素、可溶性蛋白、丙二醛(MDA)等生理指标的影响。结果表明,乙烯利与对照组(CK)相比,乙烯利在干旱胁迫下保持更高的叶片相对含水量23.5%(对照为11.5%)和更低的电解质渗透率68.1%(对照为90.7%)及MDA含量53.90μmol·g~(-1)(对照检查131.62μmol·g~(-1)),且复水后的电解质渗透率恢复程度更好;此外乙烯利处理提高了复水恢复过程草地早熟禾的可溶性蛋白含量;说明,叶片喷施乙烯利能够缓解草地早熟禾的干旱胁迫损伤,并促进干旱后的复水恢复。     

黄花苜蓿花药培养再生体系的建立与单倍体鉴定

花药组织培养再生体系的构建是单倍体育种的重要途径之一。本研究对呼伦贝尔黄花苜蓿(Medicago falcate‘Hulunbeier’)进行了花药组织培养研究并建立了花药组培再生体系。结果显示,液体悬浮培养基比固体培养基更适合呼伦贝尔黄花苜蓿花药愈伤组织的培养,其愈伤形成的培养条件为B5培养基+0.5 mg·L~(-1) 2,4-D+0.25 mg·L~(-1) 6-BA+0.4mg·L~(-1) NAA+3.0mg·L~(-1) KT;分化培养基为MS+1.0mg·L~(-1) 2,4-D+0.5mg·L~(-1) 6-BA+2%蔗糖+0.7%琼脂;生根培养基为1/2MS+0.1mg·L~(-1) NAA+2%蔗糖+0.7%琼脂;获得的再生植株经流式细胞仪鉴定,其单倍体比例高达27%。     

紫花苜蓿细胞质雄性不育恢复基因的初步定位

以紫花苜蓿(Medicago sativa)不育系MS-GN-1A为母本,恢复系MS178为父本组合构建BSA分离群体,获得的F1代均表现为雄性可育,在大田种植了221株F2代群体单株,盛花期将花粉颗粒染色后,显微镜下观察统计出,不育的F2代株数为57,可育F2代株数164,并没有观察到半不育植株。将所有植株划分为可育和不育两组,并构建可育和不育DNA混池,混池DNA从可育和不育植株组DNA中各随机抽取20个样品,以此对恢复基因定位。随机挑选160对已知的四倍体苜蓿SSR引物扩增基因池DNA,获得2个具有多态性的分子标记,分别是Mt2c12、AW166,初步定位Rf基因在类群Composite5上。将类群Composite5上的所有引物进行合成,进一步进行引物筛选,最终获得4个具有多态性的标记BI68、Mt2c12、BG267和AW776153,遗传距离分别为19.0、20.9、44.6和72.1cM。     

湖北省引入第三方检测机构提升动物产地检疫水平实践

根据规定,产地检疫中对怀疑患有检疫规程规定病种及临床检查发现其他异常情况,以及跨省调运要求实验室检测的动物,需经实验室检测合格后,官方兽医才能出具动物检疫合格证明。湖北省开展试点,引入第三方检测机构,为养殖企业提供相关动物疫病的实验室检测服务,借此提升动物产地检疫技术含量,取得了较好效果。本文介绍了湖北省引入第三方检测机构的试点情况,分析了引入第三方开展动物疫病实验室检测的意义并提出了有关思考,以期为各地提供借鉴。引入第三方检测机构,有助于提升动物产地检疫的技术水平,减少动物疫病跨区域传播风险,提升养殖企业的动物疫病防控主体责任意识,弥补动物疫病预防控制机构检测资源与能力的不足。因此,建议正确认识第三方检测机构的作用;适时修订《动物防疫法》及配套规章;借鉴国外经验,探索建立有效的第三方检测机制;将第三方检测机构的检测服务范围拓展到散养户和小型养殖企业,从而整体提升动物产地检疫水平。     

2016年江苏省猪伪狂犬病血清流行病学调查

为了解江苏省猪伪狂犬病病毒（PRV）感染和免疫情况,采用ELISA方法,对2016年采集的2 715份猪血清样品进行PRV gE和gB抗体检测。结果显示：PRV gE样品阳性率为18.49%,场点阳性率为25.07%;PRV gB样品阳性率为55.35%,场点阳性率为70.21%。结果表明：江苏省PRV野毒感染情况较严重,且免疫效果并不理想;各地市的防控效果差异较大;规模场是净化该病的关键。     

云贵川三省部分地区警犬狂犬病血清中和抗体调查

为评价警犬的狂犬病免疫效果,了解影响免疫效果的因素,2017年在云南、贵州、四川三省部分地区免疫狂犬病疫苗的警犬中随机抽样,采用荧光抗体病毒中和试验(FAVN),检测血清中狂犬病病毒中和抗体效价。结果显示,共抽检187只警犬,中和抗体阳性127只(≥0.50 IU/m L),总体免疫合格率为67.91%,其中云南、贵州、四川3省的免疫合格率分别为57.14%、68.33%、76.06%。结果分析表明,警犬狂犬病免疫合格率与犬的年龄、免疫次数、疫苗类型等因素有关,而与性别和品种无关。建议完善警犬免疫程序,加大血清中和抗体监测力度,对不合格警犬及时加强免疫;对每个影响因素进行连续性分析,以确定影响警犬狂犬病免疫合格率的具体因素。     

草鱼呼肠孤病毒生长特性研究

以草鱼呼肠孤病毒(GCRV)873毒株为试验材料,建立了GCRV荧光定量PCR检测方法;用GCRV873株感染CIK细胞及草鱼,通过对病毒RNA复制水平、子代病毒含量以及细胞病变等情况进行监测,研究其在CIK细胞及草鱼体内的生长特性。在CIK细胞中,GCRV感染后12 h即可检测到子代病毒,36 h时,所有细胞被感染;72 h时,病毒滴度达到最高,为10~(6.75) TCID_(50)/mL。在草鱼的肝、脾、肾中,均能检测到GCRV RNA,其中肾脏中含量最高,其次是肝脏,脾脏中最少。本试验为研究GCRV致病机理及制备细胞灭活疫苗奠定了试验基础。