Spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF-mediated signalling

Non-infectious prenatal mortality severely affects the porcine industry, with pathological placentation as a likely key reason. Previous studies have demonstrated that peroxisome proliferator-activated receptor gamma (PPARγ) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPARγ expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPARγ in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPARγ was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPARγ expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPARγ significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S-phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPARγ regulates placental angiogenesis through VEGF-mediated signalling.     

仙游县猪口蹄疫免疫效果检测与分析

通过对仙游县2014年秋季猪O型口蹄疫免疫抗体检测,了解和掌握仙游县猪口蹄疫免疫效果,以更好地指导今后动物疫病防控工作。     

鸡lncRNA-MSTRG.15568.9及其预测靶基因的表达

试验旨在了解在鸡睾丸中高表达的1个长链非编码RNA （long non-coding RNA,lncRNA）及其预测靶基因的时空表达规律,研究二者在鸡弱精子症中的调控作用。根据弱精子症和正常北京油鸡公鸡睾丸转录组测序筛选到的1个高表达的lncRNA （MSTRG.15568.9）,采用顺式（cis）作用模式预测其潜在靶基因SPAG4（sperm-associated antigen 4）,进一步采用实时荧光定量PCR方法进行表达量分析。分别选择3只0、5、20、30、45、60周龄正常北京油鸡公鸡,检测MSTRG.15568.9与SPAG4基因在不同周龄公鸡睾丸中的表达量差异;选择30周龄3只正常公鸡,采集睾丸、肝脏和脾脏等8个部位组织样品,检测MSTRG.15568.9与SPAG4基因在不同组织间的表达规律;选择45周龄弱精子症公鸡和正常公鸡各3只,对比MSTRG.15568.9与SPAG4基因在睾丸的表达量差异。结果显示,MSTRG.15568.9与SPAG4存在明显的时空表达差异,且二者表达趋势基本一致。在不同周龄的鸡睾丸组织中,MSTRG.15568.9和SPAG4的表达趋势相近,MSTRG.15568.9在20周龄的表达量显著高于0、5、30、45、60周龄（P<0.05）,0和5周龄表达量显著低于20、30、45和60周龄（P<0.05）;SPAG4在45周龄表达量最高,其次是20周龄（P<0.05）。MSTRG.15568.9和SPAG4在睾丸和肝脏中的表达量均显著高于脾脏、肾脏等组织（P<0.05）;在正常睾丸组织中的表达量均显著高于弱精子症睾丸组织（P<0.05）。综上所述,MSTRG.15568.9与SPAG4基因具有较明显的组织表达特异性,且MSTRG.15568.9可能调控SPAG4基因的表达,参与精子发生与精子活力调控;但其具体作用机制需要进一步探索。本研究可为鉴定与鸡弱精子症调节机制相关的功能基因提供参考。     

牛支原体和牛病毒性腹泻病毒二重二温式PCR检测方法的建立

为建立一种牛支原体(Mycoplasma bovis,MB)和牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的快速鉴别诊断方法,针对MB的uvr C基因和BVDV的5'端非编码区(5'-UTR)保守基因序列,分别设计两对特异引物,并将三温式PCR扩增程序简化为二个温度梯度,建立了鉴别MB和BVDV的二重二温式PCR方法。该方法能同时扩增MB和BVDV,扩增产物大小分别为412和170 bp。特异性试验结果显示,该方法对参试的所有毒株只扩增MB和BVDV基因组,对其它牛病原体无扩增;敏感性试验结果显示,该方法最低能同时检测到104拷贝的两种目的核酸;干扰性试验结果显示,该方法能同时检测两个模板不同浓度的组合,试验结果不受模板影响。综上,本研究所建立的二重二温式PCR方法特异、敏感、快速、简便,可应用于MB和BVDV临床鉴别诊断和流行病学调查。     

Salmonella enteritidis clearance and immune responses in chickens following Salmonella vaccination and challenge

Our previous work showed that the cell-mediated immunity (CMI) was enhanced by live Salmonella vaccine (LV). The objective of this study was to evaluate the impact of live and killed Salmonella vaccines on Salmonella enteritidis (SE) clearance and to determine if the clearance was mediated by cell-mediated and/or humoral immunity. Chickens were first immunized at 2 weeks of age followed by a booster dose at 4 weeks, challenged with live SE 2 weeks later (6-week-old) and tested for CMI, antibody response and SE clearance 1-week post SE-challenge (7-week-old). Spleen cell proliferation induced by SE-flagella and Concanavalin A (Con A) were significantly higher and SE shedding was significantly lower in the LV group. The splenic CD3 population was significantly lower and B cells were higher in the control group compared to all the SE-challenged groups (with and without vaccination). Serum antibody to SE-flagella and envelope were significantly higher in the KV group compared to all the other groups. These results suggest that LV protects against SE infection, probably by enhancing the CMI.     

广西野生动物非法利用和走私的种类初步调查

广西独特的地理位置使得广西成为全国野生动物贸易的集散地之一。为了解广西野生动物的走私种类及其季节性变化,作者统计了广西2007～2008年已破获的非法利用和走私野生动物案件涉及的种类及其季节性变化。结果表明2007和2008年分别查获案件54起和61起。野生动物走私和非法案件中涉及种类共有71种,隶属15目33科,其中两栖类2种,占所有种数的2.8%;爬行类27种（蜥蜴类8种、龟鳖类5种、鳄类1种、蛇类13种）,占38.0%;鸟类25种,占35.2%;哺乳类17种,占23.9%。2007和2008年查获的非法利用和走私野生动物分别是56种和32种,非法利用或走私的种类主要是珍稀濒危的种类,数量大,种类多。广西的野生动物走私和非法利用需要引起关注。     

一例剖腹产母犬及仔犬的护理

一头4岁龄史宾格母犬于2005年3月12日发情,3月21日和3月23日交配两次。该母犬5月21日上午出现产前征兆,扒窝,烦躁,于17点45分开始产第1头仔犬,至当晚20点50分产下第4头仔犬后,由于第5头仔犬胎位不正,至5月22日上午9点30分由兽医人工助产,仔犬死亡。10点10分产下第6头仔犬后,第7头仔犬又出现胎位不正。此时母犬体力出现下降,呼吸急促,兽医决定实施剖腹产,手术于17点20分至19点10分进行,剖腹后拿出4头仔犬,其中2头死亡,2头存活。     

汕头市猪场猪瘟和猪禽流感血清学监测报告

为了了解和掌握我市猪场的猪瘟免疫情况及感染高致病性禽流感疫病的可能性，我所分别于2005年2月和4月两次对全市范围内各类型的养猪场免疫猪瘟的猪只采集血清941份，进行猪瘟血清抗体监测和猪高致病性禽流感疫情血清学监测。现将监测结果报告如下。     

产地检疫规定病种的实验室检测能力分析

动物疫病的实验室检测是产地检疫的重要手段之一。本文分析了产地检疫51种规定检疫对象的实验室检测现状和政策需求。调查表明,目前现有法定检测依据39类,而猪肺疫等8种疫病尚无法定检测依据。北京市有14家实验室具有法定资质,仅可开展30种规定疫病检测,猪丹毒等13种疫病均无法在本市实施实验室确证。落实好基于实验室检测的产地检疫出证制度,还需增强政策操作可行性,明确实验室资质条件,完善动物疫病检测标准,加强实验室检测能力培育。     

EM在养羊业中的应用

EM是一种新的微生物制剂,在动物生产中有着广泛的应用.该文主要综述了在羊只生产中EM制剂对羊只的环境、生长、产品质量、繁殖率、疾病防治等方面的作用效果,为实际应用提供了参考.