Impact of serial activation of embryonic pax2 and WT1 genes on transdifferentiation of renal tubular epithelial cells

AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation.     

散生指天小辣椒“满天星”的选育

满天星是以辣椒胞质雄性不育系E4A为母本,恢复系E28C为父本配制的中熟辣椒一代杂种。果实羊角形,纵径6～8cm,横径1．0-1．2cm,平均单果质量4．5g。青熟果绿色,老熟果鲜红色,辣味香浓。每667m。产鲜红椒1800～2400kg,干椒300～400kg。抗CMV和疫病,中抗TMV,适宜全国各地早春露地和保护地栽培。     

xPC环境下CMAC NN在淀粉生产线液位控制中的应用

在分析CMAC神经网络的基础上,提出了利用自适应CMAC神经网络来调节淀粉生产线中乳液的液位.通过对淀粉生产线中某一液位回路进行数学建模,然后对常规PID和自适应CMAC神经网络两种控制器进行了设计与仿真.仿真结果表明,自适应CMAC神经网络在控制效果上明显优于常规PID,并且具有很高的鲁棒性.在Matlab实时开发环境xPC Target下建立了液位实时控制系统,采用快速原型控制方式,具有系统组建方便、成本低、开发周期短等特点,该系统可实现精确的过程控制.     

灯泡体支撑件对贯流泵水力性能的影响

灯泡体支撑件(包括进人孔和底部支承)是贯流泵装置内部重要的过流部件,本文采用CFD方法对贯流泵装霞内部流场进行数值模拟,对不同支撑件形状进行了比较分析和优化,并与试验结果比较.结果表明,进人孔和底部支承的形状对泵装置的水力损失和水流流态的影响都比较明显,合理的支撑件形状可以改善流态、减少水流环量,提高泵装置效率.     

基于非接触式速度测量的排种驱动系统

介绍了一种利用非接触式速度测量装置驱动排种器的工作原理.该装置用前后两个光电传感器来探测路面的反射光信号,根据两路信号的时间差得到相对速度,并用该测速装置来驱动步进电机,步进电机带动排种器转动,以达到排种和速度同步的目的.此装置可以改善现有播种机靠地轮驱动时由于打滑造成的排种不均匀的问题,使排种更加均匀化.     

Immune responses and enhanced disease resistance in Chinese drum, Miichthys miiuy (Basilewsky), after oral administration of live or dead cells of Clostridium butyrium CB2

Clostridium butyrium CB2 isolated from chickens was tested as a potential fish probiotic in the Chinese drum, Miichthys miiuy . Fish were fed live (CB), dead CB2 (D-CB) cells (10⁸ cells g⁻¹) or spent culture supernatant (SCS), for 30 days and challenged with Vibrio anguillarum or Aeromonas hydrophila . Survival was higher in both the CB and the CB-D fed groups, but the SCS group was not significantly different from the control. After feeding live or dead CB2 cells, there was increase in phagocytic activity of the head kidney macrophages, the lysozyme activity of serum and gut mucosa and immunoglobulin (Ig) level. The SCS group showed no obvious change in immune parameters. The results suggest that live or dead Clostridium butyrium CB2 has an immunomodulatory effect on fish.     

麻竹笋用林施肥效果试验

对麻竹笋用林进行施肥试验研究,分析得知,鸡粪、复合肥、配方肥3种肥料对竹笋产量和经济纯收入有显著影响,均与竹笋产量、经济纯收入呈抛物线型关系,竹笋产量、经济纯收入随施肥量增加呈现先上升后下降的趋势;3种肥料的影响程度顺序依次为：配方施肥、鸡粪、复合肥,配方施肥的最佳施肥方案为鸡400＋复60＋硫酸锌0．9。     

Dynamical analysis of net cage structures for marine aquaculture: Numerical simulation and model testing

A numerical model for analyzing dynamic properties of a net-cage system exposed in the open sea is proposed. The model is based on a lumped-mass method. In this model, the mooring lines are divided into linear elements and the net cage divided into several plane surface elements. The interconnected points or corners are called nodes or lumped-mass centers. The external force is calculated on each element and then equally distributed to its nodes. By adding the contributed forces from the neighboring elements, a system of motion equations for nodes is formed. The volume reduction coefficient of a net cage is estimated by the ratio of minimum volume of net cage during fluid and structure interaction to the original volume. In general, the numerical results are in good agreements with the experimental data. However, the results also show that if the Reynolds number is lower than the suggested range of 1400–1800, the numerical model may underestimate the environmental forces on a net-cage system.     

Comparative Study of the Adsorption Selectivity of Cr(VI) onto Cationic Hydrogels with Different Functional Groups

Two types of hydrogels with different functional groups, trimethylamine on quaternary ammonium and dimethylethoxyamine on quaternary ammonium, were synthesized. Type 1 and type 2 hydrogels were characterized with Fourier transform infrared, X-ray photoelectron spectroscopy and zeta potential analysis. The anion selectivity of these two hydrogels was investigated. The surface charges of the type 2 hydrogel were lower than those of type 1, probably because of the presence of the hydroxyl group in the ethoxy group. The Cr(VI) removal capacity of type 2 hydrogel was, therefore, less than that of type 1 hydrogel, although their adsorption rates were similar. The anion selectivity of the hydrogels was found to have a similar order: Cr(VI) > sulphate > bromide > As(V). Under the co-presence of Cr(VI) and sulphate conditions, type 2 hydrogel shows a higher selectivity towards Cr(VI). The higher hydrophobicity was caused by the presence of the ethoxy group on the quaternary ammonium in type 2 hydrogel and thus increased in selectivity towards monovalent ions (i.e. HCrO₄⁻). In addition, the hydrogels have a high reusability. Compared with type 1 hydrogel, type 2 hydrogel has an advantage for applications in Cr(VI) removal and recovery processes.     

植物EST-SSR标记开发及其应用

随着生物科技的进步,大量的植物表达序列标签（expressed sequence tags,ESTs）已经成为开发SSR标记的重要资源。EST-SSR作为一种新型分子标记,其多态性可能与基因功能直接相关,而且在相近植物间具有良好通用性,使得EST-SSR标记在实际应用中更具价值。采用计算机方法大规模发掘EST-SSR多态性位点极大地提高了SSR标记开发效率。尤其随着新一代测序技术的成熟以及测序成本的急剧下降,利用新一代测序技术产生大量的转录组数据进行EST-SSR多态性标记的计算机大规模发掘将对SSR标记的开发带来深远影响。本文简要介绍了植物EST-SSR分布特点,并对EST-SSR标记开发现状以及相关应用作了综合评述,此外,还对EST-SSR标记的计算机开发新策略进行了展望。