基于Meta分析研究毛竹入侵致土壤pH提升及养分和微生物群落结构的变化

诸多研究发现毛竹入侵周围林分提高土壤pH，但未见统计性描述报道。为此，本研究将通过大数据分析证实此现象的普遍性以及探究伴随的土壤养分和微生物变化。本研究收录包含毛竹入侵有关土壤pH变化的42篇文献总计101组数据，采用整合分析方法（Meta-analysis）进行深入探讨。在研究土壤养分变化时，本文还结合了团队采集的12个毛竹入侵带的样地数据总计92个样品18组数据进行分析。结果表明，在所有的数据组中，84.9%的土壤在经过毛竹入侵后其pH有不同幅度的提升，说明毛竹入侵周围林分普遍提高土壤pH；土壤pH增加幅度随入侵阶段、毛竹纯林时间增加而增加，随土层深度增加而降低；入侵针叶林致土壤pH增加的幅度高于阔叶林。入侵的毛竹纯林与原生林相比，总体上显著降低（P<0.05）了土壤全氮（-15.9%）、硝态氮（-21.7%）、全碳（-2.0%），却增加（P<0.05）了土壤有效磷（+54.9%）、铵态氮（+14.7%）和碱解氮（+8.2%）。对27篇包含微生物数据的文献进行整合分析，结果表明毛竹入侵改变了微生物群落结构，增加（P<0.05）了放线菌门相对丰度（+25.86%），而降低（P<0.05）了酸杆菌门（-15.49%）、浮霉菌门（-26.66%）、拟杆菌门（-22.58%）的相对丰度。本研究通过Meta分析证明毛竹入侵周围林分提高土壤pH为普遍自然现象，结合土壤硝态氮和铵态氮指标的变化推测了导致土壤pH提升的可能机制，毛竹入侵提高了土壤细菌和真菌多样性，表明入侵对土壤微生物具有正反馈效应。     

地铁车辆空调排风余能利用探讨

建立了城市轨道交通车辆的简单气流组织模型,分析了其中的空调能耗分配。对地铁车辆排风余能回收能力进行了理论计算,分析了回收方式及操作可能性。对城市轨道交通车辆空调排风潜能的开发利用有指导性意义。     

Effects of HIF-1α silencing on proliferation of rat hepatoma cells

AIM：To study the effect of hypoxia-inducible factor 1α (HIF-1α) silencing on the proliferation of hepatoma cells under hypoxia. METHODS：Rat hepatoma cell line CBRH-7919 was used in this study. Hypoxia model was established by treating the cells with cobalt chloride (CoCl2). The expression of HIF-1α was silenced by small interfe-rence RNA. Real-time RT-PCR and Western blotting were used to detect the mRNA and/or protein expression of HIF-1α, vascular endothelial growth factor (VEGF), p21 and cyclin D1 in CBRH-7919 cells under hypoxia. The proliferation of CBRH-7919 cells was measured by the technique of 5-bromo-2’-deoxyuridine (BrdU) incorporation. RESULTS：The expression of HIF-1α and VEGF at mRNA and protein levels was significantly increased under hypoxia (P<0.05). Silencing of HIF-1α significantly inhibited the expression of HIF-1α, VEGF and cyclin D1 at mRNA and/or protein levels, while increased the protein expression of p21 (P<0.05). The BrdU-positive cells in HIF-1α siRNA transfection group were significantly less than those in control group. CONCLUSION：HIF-1α silencing significantly inhibits the proliferation of hepatoma cells under hypoxia.     

我国淡水养殖业发展的空间变化影响因素分析

在陆地农业资源退化和人口总量上升的双重压迫下,淡水养殖有望成为支撑我国居民口粮供给的另一支柱产业。基于2007-2014年我国省域淡水养殖面板数据,探寻了我国淡水养殖区域的格局变化,利用全局Moran’s I指数考察淡水养殖区的空间相关性,并通过构建空间杜宾面板模型分析了产业格局变化的影响因素。研究表明:1我国淡水养殖业呈现出"东南热、西北冷"的特点,省域间的空间相关性逐年显著;2淡水养殖资金投入增加、养殖面积扩大、区域经济发展水平提升会对淡水养殖量有正向的作用,体现我国淡水养殖业由粗放经营向集约化经营的转变;3淡水养殖面积负间接效应反映"过度养殖"和环境污染对淡水资源的破坏会减缓淡水养殖业的发展;4淡水养殖业发展和城镇化的进程之间不矛盾,在未来可以稳定持续地提供淡水产品,成为国家粮食安全和食品安全重要保障。最后从合理规划淡水养殖区域格局、平衡省份间技术推广资金投入和加强对水资源污染的监管3个方面提出了推动淡水养殖业发展的政策建议。     

硒酸钠对小麦谷胱甘肽过氧化物酶活性的影响

为了解小麦硒生理机制,为小麦种质资源研究提供依据,采用硒酸钠直接喷施于土壤中的方法比较了高硒土壤(30 g/hm2硒酸钠)和低硒土壤(不施硒)上种植的不同品种小麦谷胱甘肽过氧化物酶活性。结果表明,喷施硒酸钠可明显提高小麦叶片谷胱甘肽过氧化物酶活性,不同省区小麦叶片的谷胱甘肽过氧化物酶活性不同。谷胱甘肽过氧化物酶活性不仅受土壤中硒含量的影响,还受生理和遗传等因素的限制。     

桂西北地区杉木冰冻雪灾与恢复措施

2008年1月中旬以来我国南方大部分省区遭受了历史罕见的雨雪冰冻灾害,使广西的森林资源和林业基础设施遭受惨重损失。针对广西西北部地区杉木(Cunninghamia laceolata)的受害类型和严重程度,结合杉木的生长特性和实际生产需要,提出相应的恢复技术。     

ACRL标准对农业高校大学生信息素质教育的启示

信息素质教育作为我国大学生素质教育的重要组成部分,已日益成为当前教育界、图书情报学界共同关注和讨论的重要话题。通过对ACRL《高等教育信息素质能力标准》的解析,探讨了我校大学生信息素质教育的对策建议。     

高职院校学生心理健康问题分析及教育方式选择

随着高职院校学生学习生活由普通教育向职业教育转变,发展方向由升学为主向就业为主转变,以及将直接面对社会和职业的选择.对他们的心理问题进行分析、了解其心理问题的表现及其产生原因,并作出心理健康问题的教育方式选择,既具有理论价值,又具有现实意义.     

Immunologic mechanism of CXCL10 and its receptor involved in endometriosis

AIM: To investigate the immunologic mechanism of CXC chemokine ligand 10(CXCL10) and its receptor CXC chemokine receptor 3(CXCR3) involved in the process of endometriosis (EM). METHODS: Serum samples were collected from 3 groups: EM patients without operation (n=76), EM patients with operation (n=10) and the normal control persons (n=76). CXCL10 and CA125 concentrations were detected by means of ELISA and chemiluminometry. Cell surface antigens on the activated PBMC-CD3 and CXCR3, as well as CXCR3 subgene-CXCR3A and CXCR3B were tested by flow cytometry (FC) and RT-PCR when PBMC was separated from women with EM (n=10) and without EM (n=10), and then activated. RESULTS: Serum CXCL10 concentrations between three groups were significanly different (P<0.05). Compared to normal control group, although the supernatant CXCL10 concentration and CD3+/CXCR3+PBMC number in EM group has no significant difference (P>0.05), highly expressed CXCR3B in EM group rather than CXCR3A was observed. CONCLUSION: CXCL10 in women with EM is low, indicating that it plays a vital role in the process of EM and immune system of the women with EM is defected and impaired. The immunoreactivity of PBMC from both EM patients and normal person is same to activated signal, but the productions are different: PBMC in EM group mainly express CXCR3B but PBMC in normal person mainly express CXCR3A after activation, which may be one of the immune mechanisms that EM escapes from immunological lethal effect of the infected host.