饲粮不同蛋白质、能量水平对大白鼠生长发育及体成分的影响

本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10％、15％、20％、25％和30％)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。     

Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.     

邓小平理论概论课教学内容应注意的问题

从尽量避免重复点、发现特殊教育点、认真突破难点、及时分析热点的角度分析了在正确把握高校邓小平理论教学内容上应注意的几个问题 ,以提高教学质量     

单抗捕捉ELISA检测AEV抗体方法的研究

本研究初步建立了检测ＡＥＶ抗体的单抗捕捉ＥＬＩＳＡ　方法。ＡＥＶ腹水单抗（腹水效价为１　∶１×１０６）最佳稀释度为１∶２００，ＡＥＶ抗原工作浓度为１６．０８　μｇ／ｍＬ，血清稀释度为１∶１　００，　兔抗鸡酶标抗体工作浓度为１∶５００。经阻断试验、交叉试验、重复试验，以及与进口试剂　盒　进行平行检测４０份血清，总符合率为９２．５％，表明该方法特异性强、重复性好、敏感性高，　是一种有应用前景的方法。     

氟对骨组织代谢调节因子的影响

过量氟不仅对骨细胞具有直接作用，而且可通过骨代谢调节因子（甲状旁腺素、骨钙素、降钙素）的间接作用，引起骨损害。因此，认识骨代谢调节因子，对深入探讨氟病具有重要的理论意义。     

轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

非平行直纹面的电解磨削

为解决整体叶轮扭曲叶片型面的精加工难题,本文进行了五轴联动数控展成电解磨削的基础研究.在分析平行直纹展成电解磨削整体叶轮扭曲叶片的过切误差、得到过切误差计算公式的基础上,提出用圆锥磨轮和组合式五轴联动数控方案来消除过切误差的方法.文中还介绍了数控展成电解磨床的结构与运动、经济型多轴数控系统及其联动控制方法,并建立了电解磨削非平行直纹展成曲面的数学模型,开发了五轴联动数控展成电解磨削自动数控编程系统,对航空发动机整体叶轮的叶片型面进行电解磨削.结果表明,加工效率比手工修磨、抛光提高了12倍以上.