Functions of wild stathmin 1 and the C terminal mutant

AIM: To illustrate the effects of overexpression of stathmin 1 on melanocyte growth and the relationship between C terminal motif and stathmin 1 function.METHODS: The overexpression vector and C terminal mutant coding vector were constructed. The vectors were transfected into melanocytes by Lipofectamine 2000. MTT and FCM were used to inspect the cell growth, Western blotting was a tool in caspase-3 measurement, and spectrophotography was used to detect the melanin products and tyrosinase activity.RESULTS: The vectors of overexpression and mutation were constructed and transfected into melanocytes successfully. Both of them inhibited the melanocyte growth, induced apoptosis, and decreased the melanin products and tyrosinase activity.CONCLUSION: Overexpression of stathmin 1 inhibits melanocyte growth, melanin products and tyrosinase activity. The change of C terminal motif could not affect the role of stathmin 1 in melanocyte markedly.     

甘蓝小孢子植株倍性简易鉴定方法研究

以编号为12、22的甘蓝材料（F1）经小孢子培养得到的植株为试材,先利用流式细胞仪对供试材料进行倍性鉴定,再利用普通光学显微镜观察已通过倍性鉴定的双单倍体与单倍体的气孔保卫细胞,以测定其大小与叶绿体数。结果表明：气孔保卫细胞叶绿体数与甘蓝倍性高低呈正比关系,甘蓝双单倍体气孔保卫细胞叶绿体数变异幅度为12~17,单倍体气孔保卫细胞叶绿体数的变异幅度为6~10;同一材料双单倍体与单倍体气孔保卫细胞叶绿体数的比值≈2,两者数值差异非常显著直观,易于辨别。因此观测气孔保卫细胞叶绿体数是鉴定甘蓝小孢子植株倍性的简易有效手段。     

牙鲆工厂化养殖HACCP管理模式初探

对牙鲆的工厂化养殖进行危害分析,找出其关键控制点,即水质、苗种、饲料、病害防治和运输与暂养,提出了相应的预防措施,使各关键控制点处于人为控制之下,从而确定其养殖期间日常管理的操作规程,建立了牙鲆工厂化养殖的HACCP管理模式。     

杂交中稻分蘖期干旱对产量的影响及其缓解技术研究

以杂交中籼迟熟组合宜香优300和川谷优642为材料,在海南陵水旱季大田条件下,研究了分蘖期干旱处理对中籼迟熟杂交稻产量的影响及其缓解技术。结果表明,干旱会造成水稻减产,主要原因是有效穗数和穗粒数显著降低;干旱期间平均田间持水量可作为预测产量损失度的依据;分蘖期干旱处理下,稻谷产量随着施氮量和移栽密度的增加而增加,适当提高本田施氮水平和移栽密度,可显著降低分蘖期干旱的产量损失度。     

优质稻泰优676在南靖县示范种植表现及高产栽培技术

泰优676系福建省农业科学院水稻研究所以引进广东省农业科学院水稻研究所选育的泰丰A与自育恢复系福恢676配组而成的三系弱感光籼型杂交稻,2014年通过福建省农作物品种审定。2013年南靖县开始引进试种,2015—2016年连续2 a示范种植,考察其产量表现和主要特征特性,并总结其高产栽培技术,为在南靖县的示范推广和高产栽培提供科学依据。     

USP14 regulates H2O2 induced oxidative stress in H9c2 cells

AIM:To evaluate the effect of inhibiting ubiquitin-specific protease 14(USPl4) activity on oxidative stress induced by H₂O₂ of H9c2 cells.METHODS:The H9c2 cells were incubated with H₂O₂ at 25 μmol/L for 2 h to establish the oxidative stress injury model.The cells were divided into control group,H₂O₂ group,IU1 group (25 μmol/L or 50 μmol/L) and IU1+H₂O₂ group.The H9c2 cells activity was measured by MTS assay.The level of intracellular reactive oxygen species (ROS) and cell survival rate were analyzed by flow cytometry assay.The changes of the mitogen-activated protein kinase (MAPK) family related proteins were detected by Western blot.RESULTS:Compared with control group,the cell activity and the viability rate in H₂O₂ group were decreased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were increased (P<0.05).Compared with H₂O₂ group,the cell activity and the viability rate of the H9c2 cells in IU1+H₂O₂ group were increased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were decreased (P<0.05).CONCLUSION:Inhibition of USPl4 activity reduces the oxidative stress injury of the H9c2 cells.The mechanism may be related to inhibition of the MAPK signaling and down-regulation of apoptosis related proteins.     

三角枫扦插生根过程中营养物质的动态变化

以三角枫插穗为试材,采用不同种类、浓度的生长素进行处理,分别于春夏秋3个季节进行扦插繁殖试验,并以夏插生根插穗的韧皮部为材料,研究三角枫扦插生根过程中营养物质含量的动态变化。结果表明:与对照(清水浸泡)比,用100mg·L~(-1) NAA+100mg·L~(-1) IBA浸泡插穗3h生根率最高(77%),生根指数为34.28cm;在插穗生根过程中,该处理与对照插穗韧皮部营养物质含量的动态变化趋势相似,可溶性糖含量的变化趋势为"下降-上升-下降-上升-下降"、可溶性淀粉含量为"下降-上升-下降-上升",可溶性蛋白质含量为"上升-下降-上升";100mg·L~(-1)NAA+100mg·L~(-1) IBA浸泡插穗3h明显提高了可溶性糖和可溶性蛋白质含量、促进了可溶性淀粉含量的降解转化;在缩短不定根发生周期方面,生长素处理无明显作用。     

不同枣头枝摘心方式对幼龄灰枣产量和品质的影响

以南疆地区密植灰枣为试材,采用生产上常用轻摘心、重摘心、极重摘心等3种枣头枝摘心方法,通过调查、测定枣吊数量、枣吊种类、果实单果质量、可溶性总糖、可滴定酸含量等指标,以确定一种合理的摘心方法。结果表明:枣头枝重摘心的单株坐果数显著高于轻摘心和极重摘心的单株坐果数,平均每株分别高出22.85、19.60个;重摘心后灰枣果实平均单果质量最大,为8.32g,分别比轻摘心和极重摘心灰枣果实的平均单果质量高9.5%、9.4%;枣头枝重摘心果实的可溶性总糖含量最高,为28.74%,分别比轻摘心和极重摘心后果实的可溶性总糖含量高3.86、4.13个百分点。因此,确定幼龄灰枣密植的夏季枣头枝摘心选择重摘心方式。     

贵阳市马铃薯耕层土壤养分丰缺指标建立初探

通过开阳县、修文县、息烽县在测土配方施肥项目中开展22组"3414"的肥效试验,了解氮、磷、钾缺素区马铃薯相对产量与土壤氮磷钾养分含量之间的相关关系,拟合对数方程,初步探索建立贵阳市马铃薯土壤养分丰缺指标。结果表明,以极缺乏、缺乏、适宜、最适宜、丰富、极丰富6级划分标准,马铃薯土壤有效氮丰缺指标为小于42 mg/kg、42~59 mg/kg、59~84mg/kg、84~118 mg/kg、118~168 mg/kg、大于168 mg/kg,有效磷丰缺指标为小于11 mg/kg、11~19 mg/kg、19~35 mg/kg、35~49 mg/kg、49~66 mg/kg、大于66 mg/kg,速效钾丰缺指标为小于22 mg/kg、22~43 mg/kg、43~83 mg/kg、83~192 mg/kg、192~219 mg/kg、大于219 mg/kg,均高于第次土壤普查分级标准,氮肥施用对马铃薯的增产效果优于磷肥和钾肥。