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951.
AIM:To investigate the effects of p21-activated kinase 6 (PAK6) on the invasive and migratory abilities of human non-small-cell lung cancer A549 cells. METHODS:The expression of PAK6 mRNA in A549 cells, human bronchial epithelial (HBE) cells, non-small-cell lung cancer tissues and paired adjacent non-tumor tissues was measured by real-time PCR. After A549 cells were transfected with siRNA-PAK6 (siPAK6) or negative control (NC) for 48 h, the expression of PAK6 at mRNA and protein levels was measured by real-time PCR and Western blotting, respectively. The invasion and migration of A549 cells were detected by Matrigel invasion assay and Transwell migration assay. The cytoskeletal changes were observed with FITC-phalloidin staining under confocal microscope. RESULTS:The level of PAK6 mRNA in A549 cells was higher than that in HBE cells (3.50±1.16 vs 1.12±0.42, P<0.05). The level of PAK6 mRNA in non-small-cell lung cancer tissues was higher than that in paired adjacent non-tumor tissues (5.13±1.33 vs 1.08±0.37, P<0.05). The expression of PAK6 protein decreased by 72% in A549 cells transfected with siPAK6 (P<0.05), and the level of PAK6 mRNA significantly decreased in A549 cells transfected with siPAK6 (3.72±0.75 vs 0.69±0.21, P<0.05). Matrigel invasion assay and Transwell migration assay demonstrated that knockdown of PAK6 markedly attenuated the invasion and migration of A549 cells (P<0.05). The cytoskeletal actin remodeling and reduction of stress fibers in A549 cells transfected with siPAK6 were observed under confocal microscope. CONCLUSION:PAK6 may affect the invasive and migratory abilities of non-small-cell lung cancer cells by cytoskeletal actin remodeling. 相似文献
952.
选取3年生"蓝丰"、"北陆"、"美登"蓝莓品种为试验材料,采用石蜡切片法和"指甲油法"观察了3个蓝莓品种叶片解剖结构和气孔特性。结果表明:3个品种的叶片的角质层、表皮细胞、栅栏组织、海绵组织等组织厚度都存在着差异,其中"蓝丰"的角质层、表皮、栅栏组织、海绵组织厚度最大,"北陆"次之,"美登"最小;从叶片组织紧密度来看,"美登"的最大,"北陆"次之,"蓝丰"最小;栅海比参数比较中,"北陆"最大,"美登"次之,"蓝丰"最小;从蓝莓叶片气孔特性来看,"蓝丰"气孔长度和宽度最大,"北陆"次之,"美登"最小,而气孔的密度呈现相反的结果。 相似文献
953.
为筛选出防治辣椒疫病的高效低毒新药剂,以遵辣6号为供试品种,选择68.75%银法利、58%甲霜灵猛锌、69%烯酰吗啉·锰锌、72%霜霉疫净及70%安泰生5种药剂进行防效试验,并对施药后辣椒的农艺性状和产量进行调查。结果表明,68.75%银法利、58%甲霜灵锰锌、69%烯酰吗啉·锰锌、72%霜霉疫净及70%安泰生对辣椒疫病的防治效果分别为80.12%、73.2%、59.84%、40.05%和19.88%,用药后辣椒产量分别比对照增产18.39%、15.33%、13.8%、8.05%和6.51%。这5种药剂的综合表现依次为68.75%银法利58%甲霜灵锰锌69%烯酰吗啉·锰锌72%霜霉疫净70%安泰生。 相似文献
954.
955.
GAO Qing LI Shu-ren XUN Li-ying YUAN Ke-xin XIE Yue-tao ZHANG Qian-hui HAO Qing-qing DANG Yi QI Xiao-yong 《园艺学报》2015,31(4):640-646
AIM: To investigate the effects of transplantation of bone marrow mesenchymal stem cells (BMSCs) modified by bcl-2 gene on myocardial cell apoptosis, angiogenesis and cardiac function in the rabbit after acute myocardial infarction (MI).METHODS: The rabbit BMSCs were isolated, cultured and purified in vitro. The BMSCs were transfected with adenovirus or adenovirus-Bcl-2. The rabbit model of MI was established by ligation of left anterior descending branch. The rabbits were injected with Ad-Bcl-2-BMSCs (MI+Bcl-2-BMSCs group), Ad-BMSCs (MI+BMSCs group) and DMEM (MI group) in infarction marginal zone 2 weeks after ligation. The cardiac function was evaluated by echocardiography.The apoptosis of myocardial cells was measured by TUNEL. The mRNA expression of VEGF was detected by real-time PCR. The expression of CD31 was examined by immunohistochemical staining, and new blood capillaries were counted at 4 weeks after BMSCs transplantation. The correlation of the above values with cardiac function was analyzed.RESULTS: The cardiac function was better, the apoptotic rate was lower, the mRNA expression of VEGF and the capillary density were higher in both MI+Bcl-2-BMSCs group and the MI+BMSCs group than those in MI group, and those in MI+Bcl-2-BMSCs group increased more obviously.The left ventricular ejection fraction (LVEF) had a negative correlation with the myocardial cell apoptosis rate. A positive correlation with the mRNA expression level of VEGF and the capillary density was also observed.CONCLUSION: The transplantation of BMSCs modified by bcl-2 gene significantly reduces the myocardial cell apoptosis, promotes angiogenesis, improves heart function of the rabbits with MI. 相似文献
956.
957.
云南不同花魔芋品种性状相关分析 总被引:2,自引:1,他引:2
对云南不同生态区域种植的魔花15、楚雄花魔芋2号、丽江花白魔芋、会泽花魔芋、大关花魔芋和富源花魔芋(CK)6个云南花魔芋地方品种的主要性状进行了相关性和聚类分析。分析结果表明,花魔芋种芋大小、干物质含量、球茎高等对球茎质量影响较大;干物质含量、球茎直径、球茎高等与葡甘露聚糖含量呈显著性相关;球茎质量、葡甘露聚糖、球茎直径等与干物质含量呈显著相关。云南花魔芋品种可聚为3个类群,富源花魔芋、会泽花魔芋、大关花魔芋和魔花15分布在第1个类群里,丽江花白魔芋、楚雄花魔芋2号分别聚成另外2个类群。 相似文献
958.
959.
960.
XIONG Xin-lin XIE Jian WANG Zhao-hui SU Guan-hua WANG Yan LIU Hui WANG Jue 《园艺学报》2008,24(11):2087-2091
AIM: To explore the expression and significance of fibrinogen-like protein 2 prothrombinase/fibroleukin (fgl2) in a model of rat coronary microthrombosis. METHODS: Seventy-eight Sprague-Dawley male rats were randomly assigned to model group (n=36), sham operation group (n=36) for different time (1 h, 1 d, 1week, 2 weeks, 3 weeks, 4 weeks)(n=6, respectively), and normal control group (n=6). After closing aorta, sodium laurate was injected into aortic root of the animals in model group. The animals in sham operation group were injected with normal saline for control. Microthromb were detected with HE staining and modified martius-sarlet-blue stains (MMSB). von Willebrand factor (vWF) was measured with ELISA. fgl2 mRNA and protein expressions were detected by RT-PCR and immunohistochemistry staining, respectively. RESULTS: Microthromb were observed in rat coronary microvasculature. vWF was increased in plasma. fgl2 was induced in model group compared to sham operation group (P<0.05), no significant changes were observed in sham operation group and normal control group (P>0.05). CONCLUSION: The functional disorder of endothelial cells induces the expression of fgl2 in the coronary microvasculature. fgl2 prompts the thrombosis with platelet and fibrin. 相似文献