弧菌属PCR检测方法的建立与应用

根据弧菌属细菌(Vibrio spp.)共有的rpo A基因序列,比对和设计了一对PCR引物,建立了能够快速而准确地检测弧菌属细菌的通用PCR检测方法,该方法对靶标DNA的检测灵敏度为58 fg/μL,对菌液的检测灵敏度为2.7×102cfu/m L,能够区分该属细菌与其它属的细菌,具有极高的特异性。使用建立的方法对分离自南美白对虾的病原菌进行了检验,并结合16S r DNA序列分析,结果显示待检测病原均为弧菌属的细菌,与测序结果一致,说明该检测方法可用于弧菌属细菌的检测和诊断。     

南沙群岛重点岛礁潟湖微型浮游动物的群落结构与摄食

潟湖是岛礁生物多样性演变的重要环境场,在岛礁水生态系统的物质循环和能量流动中发挥重要作用,而微型浮游动物的生态功能研究是解析南沙群岛岛礁水域潟湖生态系统初级生产力组成、流向、能量流动效率的重要研究内容之一。实验通过对南沙群岛重点岛礁渚碧礁、美济礁与永暑礁潟湖表层水域中微型浮游动物与生态环境调查,研究了其群落结构及其与环境因子的关系,并通过稀释培养实验研究了微型浮游动物的摄食压力。结果显示,调查水域共发现微型浮游动物20种,总丰度的范围为320～1 460个/L,以无壳纤毛虫丰度最高。无壳纤毛虫在渚碧礁潟湖西部水域（ZB-1）丰度最高,砂壳纤毛虫峰值则出现在永暑礁潟湖中部水域（YS-3）,桡足类幼体丰度最大值出现在美济礁潟湖北部水域（MJ-2）。聚类分析结果显示,3个岛礁潟湖中部区域的微型浮游动物群落相似度较高,溶解氧是影响群落结构的最重要因素,特别是对MJ-3站位影响最为突出。摄食实验结果显示,3个岛礁潟湖水域浮游植物生长率为0.22～1.36 d^-1;微型浮游动物摄食率范围为0.22～0.60 d^-1,微型浮游动物每天约摄食浮游植物现存量的...     

土壤含水量对茴香植株生长及精油含量和组分的影响

 采用控制浇水量的方法设计土壤(相对含水量为低44.46%±1.22%~52.84%±2.34%）、中（59.75%±3.11%~77.85%±3.47%）、高（75.24%±3.24%~99.27%±5.67%）3个范围,研究土壤含水量对茴香生长与精油含量及其组分的影响。结果表明,随着土壤含水量的升高,茴香株高、植株节数、根干质量、地上部干质量、全株干质量、全碳含量、单株精油产量和叶绿素a/叶绿素b均随之升高,而根冠比、全氮含量、蛋白氮含量、叶绿素a含量、叶绿素b含量和类胡萝卜素含量均随之下降;中土壤含水量的植株花序数、精油含量和可溶性糖含量较高,低和高土壤含水量的则较低;不同土壤含水量处理茴香植株,其精油共鉴定出22种成分,除α-蒎烯、香桧烯、β-蒎烯、γ-萜品烯、萜品油烯、肉豆蔻醚6种成分相对含量差异不显著外,其余16种成分相对含量均表现出显著或极显著差异;随着土壤含水量的增大,精油的第一主要成分反式—茴香脑含量呈升高趋势,低、中、高3个含水量处理的含量依次为41.34%、53.87%和62.97%,第二主要成分柠檬烯含量与反式—茴香脑含量变化相反,其3个含水量处理依次为44.77%、34.77%和22.16%。因此,低土壤含水量处理相对有利于茴香柠檬烯的积累,而高土壤含水量处理相对有利于反式-茴香脑的积累。     

Mechanism of mesenteric lymph reperfusion aggravates multiple organ injury in superior mesenteric artery occlusion shock rats

AIM: To explore the mechanism of mesenteric lymph reperfusion (MLR) aggravates multiple organ injury in superior mesenteric artery occlusion (SMAO) shock rats. METHODS: Male Wistar rats were randomly divided into 4 groups: in sham group, only anesthetization and operation were performed; in MLR group, occlusion of mesenteric lymphatics (ML) for 1 h followed by 2 h of reperfusion; in SMAO group, occlusion of superior mesenteric artery (SMA) for 1 h followed by 2 h reperfusion; in MLR+SMAO group, occlusion of SMA and ML for 1 h followed by 2 h of reperfusion. The homogenates of liver, kidney, myocardium and lung were prepared for determining the activities of free radical, nitric oxide, myeloperoxidase (MPO) and cell membrane ATPase. RESULTS: The MDA, NO contents and NOS, MPO activities of multiple organic homogenate in SMAO and MLR+SMAO group were higher than those in sham and MLR group, and these indexes in MLR+SMAO were increased significantly than those in SMAO group. The SOD and ATPase activities of muliple organic homogenate in SMAO and MLR+SMAO group were lower than those in sham and MLR group, and those in MLR+SMAO group was decreased obviously than those in SMAO group. CONCLUSION: The MLR enhances the multiple organ free radical injury, NO synthesis and release, PMN detention and decreases the activity of cell membrane ATPase, aggravating the major organs injury in SMAO shock rats. Intestinal lymphatic pathway plays an important role in the pathogenesis of SMAO shock.     

Overexpression of SSTR2 inhibits growth of SSTR2-positive tumors via multiple signaling pathways

AIM: To study the anti-proliferation effect of overexpressed SSTR2 in experimental cancer with different profiles of endogenous SSTRs expressions and the possible signaling pathways involved. METHODS: In the first experiment, the growth of the tumor xenografts of the inoculated capan-2 cells and A549 cells overexpressing SSTR2 or LacZ was investigated in nude mice. In the second experiment, the adenoviral vector expressing SSTR2 were introduced into experimental capan-2 xenografts by intratumoral injection. The growth inhibition of these experiment tumors was observed and the potential influences on different signaling pathways were analyzed by immunoassays. RESULTS: Overexpression of SSTR2 inhibited the growth of tumors with different profiles of endogenous SSTRs, including experimental capan-2 xenografts that had endogenous SSTR2 expression. Overexpression significantly affected a number of components in apoptotic pathway, MAPK pathway and angiogenesis. CONCLUSION: SSTR2 is a promising candidate for gene therapy in a wide spectrum of cancers.     

不同覆盖方式土壤生态效应与成龄葡萄增产效应研究

通过小区试验研究了不同覆盖保墒灌溉措施对土壤水分动态变化、土壤温度、葡萄生长状况、生理生态指标以及产量等的影响。结果表明:与对照相比,覆盖措施明显减少了土壤水分蒸发,提高了蓄水保墒作用;覆盖措施可明显提高土壤表层温度,但随深度的增大,影响程度逐渐减小;保墒处理的果树生长势强、枝条增长量大、果粒大、产量高。草帘子覆盖措施与塑料薄膜覆盖措施效果相当。     

海南安全蔬菜生产现状及发展对策

海南具有得天独厚的生态优势和非常优越的气候条件,堪称全国最大的天然温室,是我国最主要的南菜北运基地.     

甜橙新品种——桂橙一号的选育

桂橙一号是冰糖橙优良芽变株系,成熟期11月下旬,平均单果质量154.0g,大小较均匀,可食率75.66%,可溶性固形物含量14.6%,全糖12.47%,酸0.38%,风味浓郁,有蜜香,甜脆化渣,树形开张,丰产稳产,商品性好,适应性强,果实耐贮藏。     

基于Pro/Mechanica实现沙滩摩托车减速器箱体的工程分析

针对沙滩摩托车变速器箱体结构复杂的特点,使用与Pro/E无缝集成的软件Pro/Mechanica完成了箱体的工程分析。使用Pro/E建模工具完成箱体的三维建模,进入到Pro/Mechanica Structure工作环境对三维模型进行有限元分析并得到分析结果,之后使用Pro/Mechanica Thermal软件包对箱体进行温度分析,得到箱体在工作过程中的温度变化图,所有工程分析的结果都可以作为零件校核和优化设计的依据。