THE COMBINED ACTION OF 3-AMINO-1,2,4-TRIAZOLE AND AMMONIUM THIOCYANATE ON PHOSPHATASE ACTIVITY IN COTTON THISTLE (ONOPORDUM ACANTHIUM L.)

Summary. In phosphatase extracts prepared from untreated leaves of Onopordum acanthium , phosphatase activity was stimulated by amitrole (0·04 M) and ammonium thioeyanate (0·04 M), alone and together. The effect of the mixture of amitrole and ammonium thioeyanate was more than additive in experiments set up at pH 4·0, less than additive at pH 4·6 and roughly additive at pH 5·3 and 5·8. The effect of amitrole alone could be explained simply in terms of the increase in pH it effected.
In leaves harvested 4, 7 and 9 days after spraying rosettes with 1/8 lb/ac (140 g/ha) amitrole and 1/4 lb/ac (280 g/ha) ammonium thioeyanate, alone and together, more than additive or additive increases in phosphatase activity of plants sprayed with the mixture were recorded. These increases are interpreted as being due to increases in the amount of soluble protein.
Inorganic phosphate levels showed different trends with time in plants treated with amitrole alone compared with plants treated with the mixture of amitrole and ammonium thioeyanate.
These interactions between amitrole and ammonium thioeyanate are taken to indicate a possible chemical basis for the increased herbicidal activity of the mixture over amitrole alone.
Histochemical studies on the mature leaves of rosettes showed that phosphatase activity was confined to the vascular tissues while in the very young leaves it was high in the chlorenchymatous tissue and appreciable in the epidermal and hair cells.
Attention is drawn to the strong buffering action of amitrole in the pH range 4·0–5·3. This may be relevant to herbicidal activity, the vacuolar pH of the outer cells of leaves of Onopordum being in the range 4·8-5·2.
L'action combinée du 3-amino-1,2,4-triazole et du thioeyanate d'ammonium sur l'activité de la phosphatase chez Onopordum acanthium L.     

PRELIMINARY STUDIES ON THE TRANSLOCATION OF 14C-LABELLED HERBICIDES IN BRACKEN (PTERIDIUM AQUILINUM)

Summary. The translocation of ¹⁴C-labelled herbieides in field bracken has been sludied in some preliminary experiments by means of autoradiography. A detailed account is given of the methods employed. It is shown that when 2,4-D is applied to fronds at different stages of development movement of the tracer into the rhizome is considerably greater from parts of the frond which are still immature. Trasnslocation within the frond is predominantly basipetal at all stages of development; within the rhizome the direction of movement appears to be determined primarily by the nature of the vascular connections with the treated pinna.
In a comparison between the translocation of 2,4-D, 4-CPA and dalapon no difference was apparent after 24 hours, but when the treatment period was extended to 96 hours there was evidence that 4-CPA bad been translocated in a considerably greater amount than either of the other two herbicides.
Rechrches iréliminaires sur la migration des herbieides marqués am ¹⁴ C dans la fougrère algle (Pteridium aquilinum)     

THE INFLUENCE OF IRRIGATION, FERTILIZING AND MCPA ON THE COMPETITION BETWEEN SPRING CEREALS AND WEEDS

Summary. The average air-dry yield of weeds on fallow plots was about four times as large (2660 kg/ha) as the yield of weeds which developed among spring cereal crops (740 kg/ha). Application of fertilizers increased the number of weeds and their average weight on both irrigated and non-irrigated plots. Treatment with MCPA reduced the weed yield to 36% on fallow plots and to 26% on weedy cereal plots. It was more effective on fertilized plots than on unfertilized plots, but irrigation made little difference to its effectiveness. The space left by the destroyed weeds was mainly occupied by Agropyron repens . Oats were better able to compete with weeds than were wheat and barley. On unfertilized and non-irrigated plots spraying with MCPA (1-26 kg/ha) at the beginning of shooting significantly reduced the yields of weed-free barley. The yields of sprayed oats and wheat were also below the yields from the unsprayed although the reductions were not significant at the 5% level. However, on a number of fertilized and irrigated plots, both weedy and hand-weeded , on which the plants had better growing conditions, MCPA resulted in a significant increase in the grain yield of barley and oats.
Influence de l'irrigation, de la fertilisation et du MCPA sur la compétition entre céréales de printemps et mauvaises herbes     

STUDIES ON THE TRANSLOCATION IN AGROPYRON REPENS OF 14C-LABELLED 2,2-DICHLOROPROPIONIC ACID

Summary. Previous findings suggested that the translocation in Agropyron repens of 2,2-dichloropropionic acid (dalapon) may be considerably affected by the transpiration rate. This relationship has been studied further in the present investigation using ¹⁴C-labelled material and autoradiography.
It was found that when dalapon was applied to the leaves and the treated plants were placed in darkness a reduction in the transpiration rate of ca. 90% was associated with a marked increase in the amount of dalapon translocation into the roots and tillers. The evidence provided by the autoradiographs was confirmed by a quantitative assay of the ¹⁴C present in the roots. It was also found, however, that translocation was not appreciably affected either by a 50% reduction in the light intensity or when the transpiration rate was reduced by placing the plaints under conditions of high humidity in the light or by the application of white petroleum jelly to the leaves. An alternative hypothesis, namely that the effect of darkness on the movement of the herbicide might be due to changes induced in the normal pattern of assimilate translocation was also investigated but was not supported by the results obtained. Further experimentss, in which various parts of the treated shoot were placed in the dark, suggested that the effect of darkness on the translocation of dalapon is exerted primarily on the treated leaf itself.
Etudes sur to migration dans Agropyron repens de l'acide 2,2-dichloropropionique marqué avec ¹⁴C     

A PRELIMINARY STUDY OF THE COMPETITION BETWEEN KALE AND THREE WEED SPECIES OF THE GENUS POLYGONUM

Summary. The results of a pot experiment on the mutual interference between kale and three weed species are reported. The puts were sunk in soil and surrounded by kale plants so as to provide illumination conditions similar to those likely to be found in a field crop of kale. Of the three weed species, Polygonum lapathifolium appeared to be the most, and P. aviculare the least, competitive towards kale. P. persicaria was slightly less competitive than P. lapathifolium . The two latter species were much higher in dry-matter production than P. aviculare and showed greater responses to changes in the number of kale or weed plants per pot.     

Pathogenicity of Fusarium oxysporum f. sp. Cepae and its interaction with Pyrenochaeta terrestris on onion

Onion breeding lines known to be resistant to Pyrenochaeta terrestris showed, in several locations, root-pruning symptoms that have been usually associated with only the action of P. terrestris. Fusarium oxysporum f. sp. cepae was invariably isolated from affected roots and the stem-plate area of these pink-root-resistant lines. Symptoms not commonly associated with the action of Fusarium, including darkening of dry scales of yellow bulbs and a reddening of scales of white bulbs, were noted. Controlled tests were conducted to establish (a) the pathogenicity of four isolates of F. oxysporum f. sp. cepae, (b) the reaction of onion breeding lines to inoculation at various temperatures with both the pink root and the Fusarium bulb rot organism, and (c) the pathogenic interaction of F. oxysporum f. sp. cepae with P. terrestris. The Fusarium isolates were pathogenic over a wide range of temperatures (20 to 38°C.). Host reaction to the organisms in combination depended on the level of susceptibility that was the greater to one or the other organism. Resistance to one did not markedly modify susceptibility to the other when both were present. Resistance to F. oxysporum f. sp. cepae was found in several onion lines.Horticulturist, Pathologist, and Horticulturist, respectively, United States Department of Agriculture, Beltsville, Maryland, U.S.A.     

Behaviour and Socialization of the Wild Timber-Wolf

Abstract— A review article dealing with the social behaviour patterns of the wild timber-wolf, an ancestor of the domesticated dog. Investigation of these patterns may help to understand more clearly the behaviour of the dog and the influence of selection and domestication. Such behaviour patterns as flight distance, approach and avoidance behaviour, fear biting and the extinction of fear responses are discussed.
Résumé— –Article rendant compte des types de comportement social du loup des bois, ancêtre du chien domestique. Il se peut que l'étude de ces types aide à comprendre plus clairement le comportement du chien et l'influence de la sélection et de la domestication. On y discute certains types de comportement tels que la fuite, l'approche, l'esquive, l'acte de mordre sous l'effet de la peur, et la disparition des réponses à la peur.
Zusammenfassung— Ein kritischer Artikel, der sich mit den sozialen Verhaltensformen des wilden Canis occidentalis, eines Vorfahrens des Haushundes, befasst. Eine Untersuchung derselben kann dazu beitragen, das Verhalten des Hundes und den Einfluss von Zuchtwahl und Domestikation besser zu verstehen. Es werden Verhaltensformen wie Fluchtweite, Annäherungs- und Ausweichens-verhalten, Beissen aus Furcht und das Erlöschen von Furchtreaktionen diskutiert.     

A Case of Nephrolithiasis in the Dog

Abstract— A case of nephrolithiasis in a young boxer bitch is described and the surgical approach outlined.
Résumé— L'auteur décrit un cas de lithiase rénale chez une jeune chienne de race boxer et présente les grandes lignes de la thérapeutique chirurgicale.
Zusammenfassung— Es wird der Fall eines Nierensteinleidens bei einer jungen Boxerhündin beschrieben und ein Abriss des chirurgischen Eingriffs gegeben.