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91.
92.
为明确我国不同种、地理来源和毒素化学型小麦茎基腐病菌的致病力分化情况,采用纸塔法对来自全国9个省市80个采样点分离的224株小麦茎基腐病菌进行致病力分析。结果表明,不同种镰刀菌的致病力不同,黄色镰刀菌Fusarium culmorum,禾谷镰刀菌F.graminearum,假禾谷镰刀菌F.pseudograminearum及亚洲镰刀菌F.asiaticum致病力强于其他种。F.culmorum致病力显著高于F.pseudograminearum和F.asiaticum,而F.pseudograminearum,F.graminearum及F.asiaticum三者之间无显著性差异。中华镰刀菌F.sinensis,木贼镰刀菌F.equiseti,锐顶镰刀菌F.acuminatum致病力较弱,三者间苗期致病力无显著性差异;多数省份F.pseudograminearum群体间致病力无显著差异,仅山东F.pseudograminearum群体的致病性显著低于河南群体;此外,产毒类型为3ADON的F.pseudograminearum群体致病力显著高于15ADON群体。 相似文献
93.
Background
This study was conducted to investigate effect of exogenous melatonin on the development of mouse mature oocytes after cryopreservation.Results
First, mouse metaphase II (MII) oocytes were vitrified in the open-pulled straws (OPS). After warming, they were cultured for 1 h in M2 medium containing melatonin at different concentrations (0, 10−9, 10−7, 10−5, 10−3 mol/L). Then the oocytes were used to detect reactive oxygen species (ROS) and glutathione (GSH) levels (fluorescence microscopy), and the developmental potential after parthenogenetic activation. The experimental results showed that the ROS level and cleavage rate in 10−3 mol/L melatonin group was significantly lower than that in melatonin-free group (control). The GSH levels and blastocyst rates in all melatonin-treated groups were similar to that in control. Based on the above results, we detected the expression of gene Hsp90aa1, Hsf1, Hspa1b, Nrf2 and Bcl-x1 with qRT-PCR in oocytes treated with 10−7, or 10−3 mol/L melatonin and untreated control. After warming and culture for 1 h, the oocytes showed higher Hsp90aa1 expression in 10−7 mol/L melatonin-treated group than in the control (P < 0.05); the Hsf1, Hsp90aa1 and Bcl-x1 expression were significantly decreased in 10−3 mol/L melatonin-treated group when compared to the control. Based on the above results and previous research, we detected the development of vitrified-warmed oocytes treated with either 10−7 or 0 mol/L melatonin by in vitro fertilization. No difference was observed between them.Conclusions
Our results indicate that the supplementation of melatonin (10−9 to 10−3 mol/L) in culture medium and incubation for 1 h did not improve the subsequent developmental potential of vitrified-warmed mouse MII oocytes, even if there were alteration in gene expression. 相似文献94.
Fang Xiao Wei Han Yongxiu Li Yixuan Qiu Qing Sun 《The Journal of Horticultural Science and Biotechnology》2018,93(3):306-315
To study the effects of day and night temperature difference (DIF) on tomato growth, a controlled experiment using Solanum lycopersicum L., cv. Jinguan 5 was conducted. The daily mean temperature (Tm) was maintained at 18°C and 25°C, and the DIF was set at 0°C, 6°C, and 12°C. The results indicated that chlorophyll a (Chl a) and chlorophyll b (Chl b) gradually increased as DIF rose. At 18°C Tm, the carotenoid content reached a maximum at 12°C DIF. The Chl a/Chl b, net photosynthetic rate (PN), photosynthetic rate at irradiation saturation (Pmax), stomatal conductance (gs), intercellular CO2 concentration (Ci), stomatal limitation value, the maximum assimilation rate (Amax), apparent quantum efficiency (Aq), carboxylation efficiency (Ce), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), gibberellin A3 (GA3), and indole-3-acetic acid (IAA) were highest, while malondialdehyde (MDA) was lowest at 6°C DIF. At 25°C Tm, PN, Pmax, Amax, Aq, Ce, gs, Ci, CAT, POD, GA3, IAA, and zeatin reached the maximum under 6°C DIF, while SOD and MDA reached the maximum under 12°C DIF. Furthermore, the morphological index peaked at 6°C DIF under 18°C and 25°C Tm. The results suggested that 6°C DIF improved the growth and development of tomato during the flowering stage. ABBREVIATIONS: Aq – apparent quantum efficiency; Amax – the maximum assimilation rate; CAT – Catalase; Chl a(b) – chlorophyll a(b); Ca – ambient CO2 concentration; Ce – carboxylation efficiency; Ci – intercellular CO2 concentration; DIF – difference between day temperature (TD) and night temperature(TN); FM – fresh mass; gs – stomatal conductance; GA3 – gibberellin A3; IAA – indole-3-acetic acid; Ls – stomatal limitation value; MDA – malondialdehyde; Pmax – photosynthetic rate at irradiation saturation; PN – net photosynthetic rate; POD – peroxidase; ROS – reactive oxygen species; SOD – superoxidedismutase; Tm – daily mean temperature; ZT – zeatin. 相似文献
95.
Effects of road transportation on metabolic and immunological responses in Holstein heifers 下载免费PDF全文
Hyeok‐Joong Kang In Kyu Lee Min‐Yu Piao Chae‐Won Kwak Min Jeong Gu Cheol Heui Yun Hyun‐Jin Kim Hyeon‐Ju Ahn Hee‐Bal Kim Gyeom‐Heon Kim Soo‐Ki Kim Jong‐Youl Ko Jong K. Ha Myunggi Baik 《Animal Science Journal》2017,88(1):140-148
This study examined the effects of road transportation on metabolic and immunological responses in dairy heifers. Twenty Holstein heifers in early pregnancy were divided into non‐transported (NT; n = 7) and transported (T; n = 13) groups. Blood was collected before transportation (BT), immediately after transportation for 100 km (T1) and 200 km (T2), and 24 h after transportation (AT). The T heifers had higher (P < 0.05) blood cortisol and non‐esterified fatty acid concentrations after T1 and T2 than did NT heifers. By contrast, the T heifers had lower (P < 0.05) serum triglyceride concentrations after T1 and T2 than had the NT heifers. The serum cortisol and triglyceride concentrations returned (P > 0.05) to the BT concentrations at 24 h AT in the T heifers. The granulocyte‐to‐lymphocyte ratio and the percentage of monocytes were higher (P < 0.05) after T2 in the T heifers than in the NT heifers, suggesting that transportation stress increased the numbers of innate immune cells. T heifers had higher (P < 0.01) plasma haptoglobin concentrations than NT heifers 24 h AT. In conclusion, transportation increased cortisol secretion and was correlated with increased metabolic responses and up‐regulation of peripheral innate immune cells in dairy heifers. 相似文献
96.
YANG Yang XU Hou-qiang CHEN Wei ZHOU Di XU Min ZHANG Qing-qing ZHAO Huan-ping SUN Cheng-juan WANG Yuan-yuan ZHANG Ming YANG Tao 《中国畜牧兽医》2017,44(12):3401-3409
The objective of this study was to clone PDK4 and FGF10 genes, and investigate the expression level of PDK4 and FGF10 genes mRNA in different tissues of Large White pig and Congjiang Xiang pig. The PDK4 and FGF10 genes were cloned by RT-PCR and analyzed by bioinformatics, the relative expression of PDK4 and FGF10 genes were detected by Real-time PCR. The results showed that the coding region of PDK4 gene was 1 224 bp, encoding 407 amino acids; The coding region of FGF10 gene was 636 bp and encoded 211 amino acids. The homologies of nucleotide sequences of PDK4 gene with sheep, horse and human were 93%, 92% and 91%,respectively. The homologies of nucleotide sequences of FGF10 gene with sheep, cattle, human and mouse were 94%,93%, 93% and 90%, respectively. The phylogenetic tree of PDK4 gene showed that the genetic relationship of Congjiang Xiang pig, cattle and sheep were very close, the phylogenetic tree of FGF10 gene indicated that the genetic relationship of Congjiang Xiang pig, cattle, sheep, human and macaque were very close, but the genetic relationship of Congjiang Xiang pig, rat and chicken were far away. Real-time PCR results showed that, in different tissues of Congjiang Xiang pig,PDK4 gene expression in kidney tissue was higher than other tissues, with a higher expression in stomach and adipose as well,FGF10 gene expression in stomach tissue was higher than other tissues, with a higher expression in kidney and adipose as well, but both of PDK4 and FGF10 genes expression were the lowest in longissimus dorsi. In different tissues of Large White pig, both of PDK4 and FGF10 genes were expressed the highest in adipose than other tissues, PDK4 gene expression in longissimus dorsi was the lowest, while the FGF10 gene expression the lowest in heart. This study successfully cloned the PDK4 and FGF10 genes of Large White pig and Congjiang Xiang pig,and detected the relative expression of PDK4 and FGF10 genes in different tissues of Large White pig and Congjiang Xiang pig, and also provided scientific basis for further study on regulation of PDK4 and FGF10 genes on lipid metabolism and deposition. 相似文献
97.
98.
腐植酸浸种对盐碱胁迫下小麦根系抗氧化系统的影响 总被引:1,自引:0,他引:1
采用中性盐( NaCl)和碱性盐(NaHCO3)混合模拟不同浓度盐碱胁迫条件,对龙麦26和克旱16两种同基因型春小麦进行腐植酸浸种处理.分别测定根系氧离子自由基、丙二醛和谷胱甘肽含量,以及过氧化物酶超氧化物歧化酶活性,以探讨腐植酸对盐碱两种胁迫下小麦根系抗氧化系统的调控效应.结果表明:随着盐碱迫浓度的提高,小麦幼苗根系... 相似文献
99.
农户舍饲奶牛血清钙及游离羟脯氨酸水平测定 总被引:2,自引:0,他引:2
采用氯胺T比色法和EDTANa_2滴定法,对重庆市农户舍饲奶牛不同泌乳阶段血清中游离羟脯氨酸与血清钙的水平进行 测定,旨在调查试验条件下奶牛血钙代谢情况以及骨营养不良发生的可能性。结果显示: 在农户全舍饲条件下,血清钙含量Ⅱ组显著低于Ⅴ组、Ⅵ组,其它各组间差异不显著;Ⅰ~ Ⅵ组血清羟脯氨酸含量分别为2.32±0.37μg/ml、2.36±0.59μg/ml、2.02±0.54μg/ml、1.90±0.44μg/ml、1.98±0.45μg/ml、1.79±0.18μg/ml,Ⅰ、Ⅱ组与Ⅵ组差异极显著。采样后2-3个月内进行临床观察,血清游离羟脯氨酸水平与骨营养不良发生率呈现一致性变化。而钙磷摄入不足,比例不当是主要原因。 相似文献
100.
一种昆虫病原线虫共生菌的分离鉴定及致病性研究 总被引:1,自引:0,他引:1
从广东省阳江县的土壤中,分离到一种病原斯氏线虫(Steinernema sp.8503),从线虫3龄幼虫体,又分离出8503菌株。此菌为共生菌,革兰氏阴性、非孢子型杆菌,2.0~12.0×0.6~1.3微米,鞭毛为周毛,硝酸盐不产生亚硝酸盐,过氧化氢酶阴性,脲素酶阴性,磷酸脂酶阴性,卵磷酯酶阳性,七叶苷水解阳性,不具荧光,对昆虫有致病性,从一系列的生化特性和线虫的带菌率、细菌囊和细菌的形状、分布等检查,此菌为大肠杆菌科的Xenorhabdus nemalophilus。对促进线虫在人工培养基的繁殖,提高线虫的产量和毒力均有一定的价值。 相似文献