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81.
“义乌盆地”的地理和气候易造成污浊空气在城市内沉积,也不利于外围新鲜空气进入城区。义乌在建设了大量的城市防护绿地的同时,选择了一条独特的宏观生态途径,理顺和打通了城市进气和排气通道,并进一步提出完善城市防护绿地宏观生态体系的几点举措。 相似文献
82.
整合位点的侧翼序列是外源基因表型研究和功能探讨的前提条件,对目的基因的正常转录和表达有着重要影响。本试验利用热不对称交措式PCR(Thermal Asymmetric Interlaced PCR,TAIL-PCR)的方法克隆了目的基因。结果表明:外源基因整合到牛的12号染色体基因组克隆(NW_003104315.1)中。分析了整合位点的纯合性发现转基因牛为整合位点杂合子。TAIL-PCR法能够有效、快速的鉴定外源基因在基因组中的整合位置,具有良好的应用前景。 相似文献
83.
84.
The mechanism that controls the proportion of cannabichromene (CBC), a potential pharmaceutical, in the cannabinoid fraction
of Cannabis sativa L. is explored. As with tetrahydrocannabinol (THC) and cannabidiol (CBD), CBC is an enzymatic conversion product of the precursor
cannabigerol (CBG). CBC is reported to dominate the cannabinoid fraction of juveniles and to decline with maturation. This
ontogeny was confirmed in inbred lines with different mature chemotypes. A consistent CBC presence was found in early leaves
from a diverse clone collection, suggesting that CBC synthase is encoded by a fixed locus. Morphological variants possessing
a ‘prolonged juvenile chemotype’ (PJC), a substantial proportion of CBC persisting up to maturity, are presented. PJC is associated
with a reduced presence of floral bracts, bracteoles, and capitate-stalked trichomes. Genetic factors causing these features
were independent of the allelic chemotype locus B that was previously postulated and regulates THC and CBD synthesis and CBG accumulation. In contrast to previously described
Cannabis chemotypes, the cannabinoid composition of PJCs showed plasticity in that reduced light levels increased the CBC proportion.
The ability of PJC plants to enable the production of pharmaceutical raw material with high CBC purity is demonstrated. 相似文献
85.
A major factor affecting spring canola (Brassica napus) production in Canada is killing frosts during seedling development in the spring and seed maturation in the fall. The objective
of this study was to explore the possibility of producing spring canola lines with mutations that have altered biochemical
pathways that increase cold tolerance. The approach was to generate UV point mutations in cultured microspores followed by
chemical in vitro selection of individual mutant microspores or embryos resulting in measurable alterations to various biochemical
pathways with elevated levels of key defense signaling molecules such as, salicylic acid (SA), p-Fluoro-d,l-Phenyl Alanine (FPA), and jasmonic acid (JA). In addition, since proline (Pro) is known to protect plant tissues in the cold-induced
osmotic stress pathway, mutants that overproduce Pro were selected in vitro by using three Pro analogues: hydroxyproline (HP),
azetidine-2-carboxylate (A2C); and, 3,4-dehydro-d,l-proline (DP). Of the 329 in vitro selected mutant embryos produced, 74 were identified with significant cold tolerance compared
to their donor parents through indoor freezer tests at −6°C, and 19 had better winter field survival than winter canola checks. All chemically selected mutant doubled haploids with increased
cold tolerance compared well with parent lines for all seed quality and agronomic parameters. Development of increased frost
tolerant cultivars should allow for spring canola to be produced in western Canada without compromising seed quality. 相似文献
86.
Paul C. Stoy Mathew Williams Mathias Disney Ana Prieto-Blanco Brian Huntley Robert Baxter Philip Lewis 《Landscape Ecology》2009,24(7):971-986
Transferring ecological information across scale often involves spatial aggregation, which alters information content and
may bias estimates if the scaling process is nonlinear. Here, a potential solution, the preservation of the information content
of fine-scale measurements, is highlighted using modeled net ecosystem exchange (NEE) of an Arctic tundra landscape as an
example. The variance of aggregated normalized difference vegetation index (NDVI), measured from an airborne platform, decreased
linearly with log(scale), resulting in a linear relationship between log(scale) and the scale-wise modeled NEE estimate. Preserving
three units of information, the mean, variance and skewness of fine-scale NDVI observations, resulted in upscaled NEE estimates
that deviated less than 4% from the fine-scale estimate. Preserving only the mean and variance resulted in nearly 23% NEE
bias, and preserving only the mean resulted in larger error and a change in sign from CO2 sink to source. Compressing NDVI maps by 70–75% using wavelet thresholding with the Haar and Coiflet basis functions resulted
in 13% NEE bias across the study domain. Applying unique scale-dependent transfer functions between NDVI and leaf area index
(LAI) decreased, but did not remove, bias in modeled flux in a smaller expanse using handheld NDVI observations. Quantifying
the parameters of statistical distributions to preserve ecological information reduces bias when upscaling and makes possible
spatial data assimilation to further reduce errors in estimates of ecological processes across scale. 相似文献
87.
88.
Robert A. Mccleery 《Landscape Ecology》2009,24(4):483-493
Predator stimuli created by humans in the urban environment may alter animals’ anti-predator behaviors. I hypothesized that
habituation would cause anti-predator behaviors to decrease in urban settings in response to humans. Additionally, I hypothesized
that populations habituated to humans would show reduced responses to other predator stimuli. I observed three populations
of squirrels (urban, suburban and rural) responses to human approaches, red-tailed hawk vocalizations (Buteo jamaicensis) and coyote (Canis latrans) vocalizations. Mahalanobis distances of anti-predator behaviors in response to human approaches were consistent with the
urban–rural gradient. Flight initiation distances (X
2 = 26.33, df = 2, P < 0.001) and amount of time dedicated to anti-predator behavior (X
2 = 10.94, df = 2, P = 0.004) in response to human approaches were also consistent with the urban–rural gradient. Supporting the habituation hypothesis,
naive juvenile squirrels increased flight initiation distances (X
2 = 35.89, df = 1, P < 0.001) and time dedicated to anti-predator behaviors (X
2 = 9.46, df = 1, P = 0.002) relative to adult squirrels in the same urban environment. Time dedicated to anti-predator behaviors differed among
all three sites in response to both coyote (X
2 = 9.83, df = 2, P = 0.007) and hawk (X
2 = 6.50, df = 2, P = 0.035) vocalizations. Responses to both vocalizations on rural sites (coyote = 45%, hawk = 55%) greater than twice that
found on the urban sites (coyote = 11%, hawk = 20%). This is possibly the first case of a transfer of habituation demonstrated
under field conditions. 相似文献
89.
Estimation of actual evapotranspiration for a drip-irrigated Merlot vineyard using a three-source model 总被引:3,自引:3,他引:0
A study was performed in order to evaluate the three-source model (Clumped model) for direct estimation of actual evapotranspiration
(ETa) and latent heat flux (LE) over a drip-irrigated Merlot vineyard trained on a vertical shoot positioned system (VSP) under
semi-arid conditions. The vineyard, with an average fractional cover of 30%, is located in the Talca Valley, Region del Maule,
Chile. The performance of the Clumped model was evaluated using an eddy covariance system during the 2006/2007 and 2007/2008
growing seasons. Results indicate that the Clumped model was able to predict ETa with a root mean square error (RMSE), mean bias error (MBE), and model efficiency (EF) of 0.33, −0.15 mm day−1 and 74%, respectively. Also, the Clumped model simulated the daytime variation of LE with a RMSE of 36 W m−2, MBE of −8 W m−2, and EF of 83%. Major disagreement (underestimated values) between observed and estimated values of ETa was found for clear days after rainfall or foggy days, but underestimated values were less than 10% of the data analysis.
The results obtained in this study indicate that the Clumped model could be used to directly estimate vine water requirements
for a drip-irrigated vineyard trained on a VSP. However, application of the Clumped model requires a good characterization
of the drip-irrigated vineyard architecture. 相似文献
90.
针对电液伺服遥操纵机器人主-从双向伺服位置控制中存在的非线性和不确定性,提出主-从侧分别使用带干扰观测器的最优控制和有鲁棒补偿的PD反馈控制算法,以克服主动侧各液压缸因动态特性差异造成的位置跟随误差和从动侧外界环境的随机干扰对系统稳定性的影响.分别设计了观测器跟踪扰动的二次型控制器和有动态鲁棒补偿的位置控制器,通过仿真和试验验证了所设计控制器的有效性.试验结果表明,所设计的位置控制器,既提高了主-从位置跟随精度,又具有较强的自适应性和鲁棒性;提高了力反馈电液伺服遥操纵机器人系统的操作性. 相似文献