Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

The Role of Hepatic Ultrasonography in Small Animal Medicine: From the Identification of the Lesions to Diagnosis

Veterinary Research Communications -     

污水污物潜水旋流泵性能及设计方法试验研究

对影响潜水旋流泵性能的6个主要过流件几何参数进行了试验研究，初步掌握了旋流泵叶轮及泵体主要几何尺寸对性能影响的规律。在分析16种优秀水力模型的基础上，计算出叶轮D2及b2函数回归方程式，并用设计实例进行检验，样机效率比国内外同类产品提高3％左右。     

Enhanced recovery of avian influenza virus isolates by a combination of chicken embryo inoculation methods.

Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.     

Detection of feline coronavirus infection in captive cheetahs (Acinonyx jubatus) by polymerase chain reaction.

Feline coronavirus genetic elements were detected by polymerase chain reaction from blood, fecal samples, and effusive fluid collected from 33 cheetahs in the U.S.A. Feline coronavirus-specific serum antibodies were also measured by indirect immunofluorescence. Ten cheetahs were positive for viral shedding by polymerase chain reaction, whereas 13 were seropositive by immunofluorescence. Results of serology did not consistently correlate with shedding of virus, and the capture antigen used for detection of feline coronavirus-specific antibodies had a significant impact on results. Testing of samples from one population over a 1-yr period indicated chronic infection in some animals. These relatively healthy carrier animals were a source of virus for contact animals. Screening programs in cheetah populations for feline coronavirus infection may be most reliable if a combination of serologic analysis and viral detection by polymerase chain reaction is used.     

寿光蔬菜叫响"文化品牌"

王高银刹、仓颉双井、圣城古槐等寿光古代及现代的知名景观,全部“搬进”了第六届菜博会的土展厅,不过组成景点的不是土木砖瓦,而是清一色的蔬菜瓜果。这些普通蔬菜瓜果的独到组合,不仅组成了令人留连的“蔬菜大观园”,而且展现出“菜乡”寿光传统的民俗文化和蔬菜产业深厚的文化底蕴。寿光蔬菜种植历史悠久,北魏农学家贾思勰就诞生在这里,明清年间,“独根红”韭菜被确定为宫廷贡品。为叫响蔬菜的“文化品牌”,     

P‐20 Clinical,cytopathological and histopathological evaluation of sporotrichosis in experimentally infected cats

The purpose of this study was to evaluate the clinicopathological aspects of experimental sporotrichosis in cats and compare the sensitivity of cytopathology, histopathology and culture as diagnostic tools in different phases of the infection. Twenty adult, mixed‐breed cats (10 males and 10 females) were inoculated subcutaneously with 10⁶ fungal microorganisms. Clinical examination was performed weekly. Cytopathologic, histopathologic and culture examinations were performed at 15, 30 and 60 days postinoculation. Culture of multiple organs was performed after euthanasia at 30 (10 cats) and 60 (10 cats) days postinoculation. Friedman parametric and nonparametric statistical analysis were applied to the results. The nodular, tumoral and necrotic lesionsprogressed significantly until day 30 postinoculation, and partial spontaneous regression occurred at day 60, particularly in males. An intense inflammatory pyogranulatomous and lymphocytic infiltrate with rare giant cells and sparse fibrosis associated with numerous, pleomorphic, intra‐ and extracellular fungal cells were observed on day 30. These findings gradually decreased by day 60. Despite the inflammatory granuloma associated with feline sporotrichosis, a tendency for dissemination was observed, with fungal isolation in the lymph nodes, spleen and liver at the 30 and 60 days postinoculation. No significant differences were observed between cytopathology, histopathology and fungal culture during the different phases of the disease. Therefore, cytological examination was considered a simple, rapid and inexpensive diagnostic method at all stages of this disease. Funding: Self‐funded.     

Localization of extracellular matrix receptors in ICGN mice, a strain of mice with hereditary nephrotic syndrome.

Fibrotic degeneration was examined in the kidneys of ICR-derived glomerulonephritis (ICGN) mice, a novel inbred mouse line with a hereditary nephrotic syndrome of unknown etiology considered to be a good model of human idiopathic nephrotic syndrome. In the present study, we histochemically revealed changes in accumulation of extracellular matrix (ECM) components and in localization of integrins, cellular receptors for ECM, in the kidneys of ICGN mice with the progression of renal failure. Excessive accumulation of basement membrane (laminin and collagen IV) and interstitial (type III collagen) ECM components were demonstrated in the glomeruli and tubulointerstitum of ICGN mice. Marked deposition of type I collagen and tenascin was seen only in the glomeruli of ICGN mice but not in those of ICR mice as normal controls. Increased expression of integrin alpha1-, alpha2-, alpha5- and beta1-subunits in glomeruli with fibrotic degeneration and abnormal distribution of alpha6-subunit were noted in the kidneys of ICGN mice. Excessive laminin, a ligand of alpha6beta1-integrin, was demonstrated on the tubular basement membrane, but alpha6-subunit diffusely disappeared on the basal side of the tubular epithelial cells. We presumed that abnormal integrin expression in renal tubules causes epithelial cell detachment, and consequently tubular nephropathy, and results in disorder of ECM metabolism causing excessive accumulation of ECM components in the kidneys of ICGN mice.