蔷薇属植物的胞核学研究及其展望

对蔷薇属植物的胞核学研究历史、基于染色体的分类、系统研究及现代月季的形成过程进行了概述,总结了蔷薇属植物的染色体数目、核型研究现状及荧光原位杂交技术在蔷薇属植物胞核学研究中的应用。对蔷薇属的胞核学研究进行了展望。     

香泽兰总黄酮对斑马鱼脾脏毒性病理组织学作用

为了探索香泽兰总黄酮对斑马鱼脾脏显微组织结构的毒性作用,采集香泽兰全草,用70%乙醇(v/v)浸泡后,辅以超声提取,再经大孔树脂柱层析纯化,浓缩,冰冻干燥,制得香泽兰总黄酮冻干粉,用此总黄酮冻干粉按不同浓度对斑马鱼进行水触媒染毒,21 d后取脾脏,制作石蜡切片,苏木精-伊红染色,显微观察。结果发现,随着香泽兰总黄酮水触媒染毒浓度的增加,供试斑马鱼脾脏显微组织结构出现了不同程度的病变,主要表现含铁血黄素沉着增多,脾窦扩张,脾脏内淋巴细胞增多,血细胞核浓缩、溶解,甚至导致脾脏坏死。说明一定浓度的香泽兰总黄酮对斑马鱼脾脏具有一定的毒性作用。     

厌氧降解条件下秸秆育苗容器降解性能研究

为探讨秸秆育苗容器在厌氧条件下的生物可降解性能,以厌氧发酵罐中的沼渣为接种物,微晶纤维素和聚丙烯(PP)为阳性和阴性对照,利用排水法测定参试样品的总产气量,采用气相色谱测定甲烷和二氧化碳的产生量。结果表明:4周内,粉状和片状秸秆育苗容器降解率分别达到了27.5%和23.0%,粉状秸秆育苗容器甲烷和二氧化碳产生量大于片状秸秆育苗容器,表明秸秆育苗容器在厌氧条件下具有一定的生物降解性能。     

Crop Yield Forecasted Model Based on Time Series Techniques

Traditional studies on potential yield mainly referred to attainable yield: the maximum yield which could be reached by a crop in a given environment. The new concept of crop yield under average climate conditions was defined in this paper, which was affected by advancement of science and technology. Based on the new concept of crop yield, the time series techniques relying on past yield data was employed to set up a forecasting model. The model was tested by using average grain yields of Liaoning Province in China from 1949 to 2005. The testing combined dynamic n-choosing and micro tendency rectification, and an average forecasting error was 1.24%. In the trend line of yield change, and then a yield turning point might occur, in which case the inflexion model was used to solve the problem of yield turn point.     

海兰鸡IL-18全基因的克隆与序列分析

IL-18是一种能诱导产生IFN-γ的新型细胞因子,在调节Th1型细胞免疫应答中起重要作用。根据GenBank发表的鸡IL-18cDNA基因序列,自行设计一对引物,经植物血凝素(PHA)活化60日龄海兰鸡的脾淋巴细胞后,提取其总RNA,经反转录-聚合酶链反应(RT-PCR)扩增,扩增产物进行了T-A克隆、测序,获得了中国海兰鸡IL-18基因全序列,其大小为597 bp,与GenBank中查得的鸡IL-18基因进行比较发现,中国海兰鸡IL-18基因与Schneider报道的鸡IL-18基因序列完全一致,为进一步研究鸡IL-18基因表达、生物学活性和应用奠定了基础。     

Cytogenetics and molecular genetics of patients with disorder of sex deve-lopment

AIM: To explore the cytogenetics and molecular genetics of the patients with disorder of sex deve-lopment. METHODS: Three patients of 46, XX male sex-reversal syndrome and 1 patient of female pseudoherma-phroditism received gene copy number detection of sex-determining region Y gene (SRY), CYP21A2, DSS, DAX1, WNT4, SOX9, NR5A1 by multiplex ligation-dependent probe amplification (MLPA). Fluorescence in situ hybridization (FISH) analysis was also performed to map the genes using the probes from the bacterial artificial chromosome (BAC) clone. RESULTS: The single-copied SRY gene was detected by MLPA among the 3 patients of 46, XX male sex-reversal syndrome. FISH analysis revealed that there were double X chromosomes, and SRY gene was translocated to the short arm of one of the X chromosomes. Chromosome analysis of the mother of the female pseudoherma-phroditism patient was 46, XX, whose MLPA analysis showed loss of heterozygosity at CYP21A2-ex03 and amplification at CYP21A1P-ex02. Chromosome analysis of her father was 46, XY, whose MLPA analysis revealed loss of heterozygosity at CYP21A2-ex01 and CYP21A2-ex03,and amplification at CYP21A1P-ex02 and CYP21A1-ex10. CONCLUSION: Sex determination is a process controlled by SRY gene and other genes also participate in. The copy number detection of SRY gene and other genes by MLPA contributes to determine the etiology of the patients with disorder of sex development.     

镉胁迫下构树qRT-PCR内参基因筛选及验证

目的 筛选出镉（Cd）胁迫下构树中稳定表达的内参基因,为后续开展构树耐Cd的分子机理研究奠定基础。 方法 以Cd胁迫下构树的根和叶组织为材料,通过实时荧光定量PCR（qRT-PCR）检测10个候选基因（BpUBE2、BpRPL8、BpActin、BpGAPDH、BpHSP、BpTUA、BpDOUB、Bpβ-TUB、BpHIS、BpNADH）的表达情况,利用geNorm、NormFinder、BestKeeper和RefFinder分析软件对候选内参基因的表达稳定性进行综合评估,并通过逆境胁迫响应基因BpDREB验证筛选出的内参基因的稳定性。 结果 BpDOUB和BpNADH在Cd胁迫下构树中基因表达相对稳定,BpGAPDH表达稳定性最差。以BpDREB验证内参稳定性时发现,单独或组合使用BpDOUB及BpNADH为内参基因时,BpDREB基因表达水平显示出相似的趋势,而稳定性较差的BpGAPDH基因未能对BpDREB的表达量进行准确校正。 结论 BpDOUB、BpNADH基因以及BpDOUB + BpNADH基因组合均可作为合适的内参基因,提高Cd胁迫下构树相关基因表达分析的可靠性。     

微囊藻毒素及其生殖毒性的研究进展

工业化、城市化的发展以及农业生产中大量化肥的使用,使自然界许多水体的污染逐渐加重,出现富营养化现象,导致藻类尤其是蓝藻的过度繁殖生长,不仅破坏了水生生态系统,而且对经济和社会发展、环境以及人类健康的影响很大[1-6]。蓝藻广泛地生长在世界各地的水体中,某些种类的蓝藻     

天津地区饲用高粱属作物品种生长适应性比较

为筛选适宜天津地区种植的饲用高粱属作物品种,于2019年在天津市宁河区实验林场选择了21个饲用高粱属作物品种,进行物候期观测、农艺性状比较及在抽穗期收获后测定鲜草产量及干草产量.结果 表明:参试的饲用高粱属作物品种的物候期有显著差异,其中晋牧1号、晋牧3号的生育期最短,光明星、甘露400的生育期最长.辽甜13号的株高最...     

雏鸡肠组织β1基因的鉴定及时空表达特性

设计1对引物,建立RT—PCR方法对β1基因mRNA进行鉴定;以GAPDH基因为内参,建立检测鸡β1基因表达水平的sYBRGreenI实时荧光定量PCR方法,并用此方法测定1～20日龄雏鸡不同肠段组织81基因表达水平。结果显示,从鸡肠组织成功鉴定出β1基因mRNA;检测内参基因GAPDH和目的基因β1的实时荧光定量PCR方法扩增效率一致（扩增曲线斜率差〈0．1）,满足使用比较Ct值法对B1基因mRNA进行相对定量分析的前提;扩增曲线、熔解曲线及凝胶电泳结果提示该方法特异性好。实时荧光定量PCR检测结果表明,1日龄雏鸡十二指肠和盲肠、10日龄雏鸡空肠和直肠、5日龄雏鸡回肠组织中β1基因相对表达量最高。结果表明,鸡肠组织中存在β1基因表达,并在不同日龄不同肠段表达水平存在差异。