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141.
Yuru Luo Ruiqi Zhang Jing Gao Yali Wang Weimin Zhang Suzhu Qing 《Reproduction in domestic animals》2020,55(7):822-832
Epidermal growth factor (EGF) is one of the important regulatory factors of EGF family. EGF has been indicated to effectively inhibit the apoptosis of follicular cells, to promote the proliferation of granulosa cells and the maturation of oocytes, and to induce ovulation process via binding to epidermal growth factor receptor (EGFR). However, little is known about the distribution and expression of EGF and EGFR in cattle ovary especially during oestrous cycle. In this study, the localization and expression rule of EGF and EGFR in cattle ovaries of follicular phase and luteal phase at different time points in oestrous cycle were investigated by using IHC and real-time qPCR. The results showed that EGF and EGFR in cattle ovary were mainly expressed in granulosa cells, cumulus cells, oocytes, zona pellucida, follicular fluid and theca folliculi externa of follicles. The protein and mRNA expression of EGF/EGFR in follicles changed regularly with the follicular growth wave both in follicular and in luteal phase ovaries. In follicular phase ovaries, the protein expression of EGF and EGFR was higher in antral follicles than that of those in other follicles during follicular growth stage, and the mRNA expression of EGFR was also increased in stage of dominant follicle selection. However, in luteal phase ovaries, the growth of follicles was impeded during corpus luteum development under the action of progesterone secreted by granular lutein cell. The mRNA and protein expressions of EGF and EGFR in ovarian follicles during oestrous cycle indicate that they play a role in promoting follicular development in follicular growth waves and mediating the selection process of dominant follicles. 相似文献
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LI Ming JIA Hong XIN Ting GUO Xiao-yu YUAN Wei-feng HOU Shao-hua HOU Qiang GAO Xin-tao WU Jing DONG Rui-kai YANG Hong-jun LIU Lai-xing ZHU Hong-fei 《中国畜牧兽医》2015,42(10):2544-2550
To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis. 相似文献
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为了给北方海水鱼虾蟹类育苗增添一种新的生物活饵料,利用循环水培养装置研究了西藏拟溞Daphniopsis tibetana Sars在稀释海水(盐度为18~20)中规模化培养的技术工艺参数,即起始放养密度、充纯氧和日采收率。结果表明:起始放养密度对西藏拟溞种群增长和日产量影响显著(P0.05),不同起始放养密度(200、550、900、1250 ind./L)培养试验中,放养密度为1250ind./L时,培养第33天时种群密度达最高,为(9500±550.73)ind./L,平均日产量为(125.00±8.35)g/(m3·d);充纯氧与充空气相比较,充纯氧能显著提高种群密度和日产量(P0.05),当放养密度为1250 ind./L、充纯氧时,西藏拟溞种群密度最高达(11 600±560.45)ind./L,平均日产量为(156.82±8.49)g/(m3·d);日采收率对西藏拟溞的种群增长和日产量也有显著影响(P0.05),日采收率为20%时最佳,放养密度为1250ind./L、日采收率为20%时,西藏拟溞种群密度最高为(12 900±995.04)ind./L,平均日产量为(176.52±15.08)g/(m3·d)。 相似文献
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