全文获取类型
收费全文 | 129007篇 |
免费 | 6666篇 |
国内免费 | 133篇 |
专业分类
林业 | 5367篇 |
农学 | 3411篇 |
基础科学 | 823篇 |
13673篇 | |
综合类 | 27512篇 |
农作物 | 5081篇 |
水产渔业 | 5400篇 |
畜牧兽医 | 65841篇 |
园艺 | 1395篇 |
植物保护 | 7303篇 |
出版年
2018年 | 1511篇 |
2017年 | 1662篇 |
2016年 | 1485篇 |
2015年 | 1277篇 |
2014年 | 1625篇 |
2013年 | 4161篇 |
2012年 | 3044篇 |
2011年 | 3713篇 |
2010年 | 2358篇 |
2009年 | 2432篇 |
2008年 | 3691篇 |
2007年 | 3480篇 |
2006年 | 3356篇 |
2005年 | 3220篇 |
2004年 | 3088篇 |
2003年 | 3215篇 |
2002年 | 3009篇 |
2001年 | 3510篇 |
2000年 | 3519篇 |
1999年 | 2800篇 |
1997年 | 1149篇 |
1995年 | 1286篇 |
1993年 | 1197篇 |
1992年 | 2557篇 |
1991年 | 2853篇 |
1990年 | 2800篇 |
1989年 | 2794篇 |
1988年 | 2595篇 |
1987年 | 2608篇 |
1986年 | 2731篇 |
1985年 | 2626篇 |
1984年 | 2114篇 |
1983年 | 1879篇 |
1982年 | 1326篇 |
1981年 | 1269篇 |
1980年 | 1186篇 |
1979年 | 2049篇 |
1978年 | 1642篇 |
1977年 | 1445篇 |
1976年 | 1294篇 |
1975年 | 1467篇 |
1974年 | 1941篇 |
1973年 | 1890篇 |
1972年 | 1964篇 |
1971年 | 1847篇 |
1970年 | 1796篇 |
1969年 | 1628篇 |
1968年 | 1381篇 |
1967年 | 1491篇 |
1966年 | 1312篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
181.
The phenomenon of RNA interference (RNAi) is highly conserved mechanism in the organism evolution. As a immune system, RNAi is a ubiquitous mechanism against invading microorganism in plant and animal cells. Recently, it has been found that RNAi is the process by which double-strand RNA(dsRNA) directs sequence-specific degradation of messenger RNA and the mediations of sequence specific messenger RNA degradation are 21-and 23-nucleotide small interfering RNAs that generate by ribonuclease from endogenous longer dsRNA or by transfectious technics from heterologous dsRNA. Over the past few years, the way in which cells respond to dsRNA by silencing homologous genes has revealed a new regulating paradigm in biology. 相似文献
182.
The vulvar labia of ewes naturally infected with ureaplasma were significantly more swollen and red than those of uninfected ewes. Similar changes were observed following experimental infection of previously uninfected ewes. These differences, although statistically significant, were not sufficiently marked to be useful in diagnosing infection by clinical examination. 相似文献
183.
The data obtained in the workshop provide further evidence that CH128A and IL-A26 and the 12 new mAbs that form a cluster recognise the bovine orthologue of CD2. The mAbs inhibit rosetting with SRBC, stain cells in primary and secondary lymphoid organs in patterns consistent with those obtained in humans with anti-CD2 mAbs, and the 11 IgG mAbs all immunoprecipitate a peptide with a Mr of 58-62 kDa. It is not clear from the studies whether the epitopes defined by the mAbs correspond with the region I and II epitopes present on CD2. None of the data suggest that any of the mAbs recognise the region III (CDD2R) epitope (Peterson and Seed, 1987; Knapp et al., 1989). Further studies are now needed to define the physical and functional relation of the epitopes and establish whether antibody-mediated activation corresponds with that noted in humans. Data reported in one study (Baldwin et al., 1988) with IL-A26 suggest possible differences in the requirements for activation. In addition, further studies are needed to demonstrate how many cell types express BoCD2. In mice, evidence has been presented which shows the mouse orthologue is expressed on some B cells (Yagitta et al., 1989). Studies in cattle have clearly shown CD2 is present on the majority of CD4+ and CD8+ T-cells and a small population of CD4-/CD8- cells (Baldwin et al., 1988; Davis, unpublished observations). Evidence presented in this workshop has shown that some CD2+ cells express a WC2 molecule (Sopp et al., 1991).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
184.
E M Aburto A Cribb I C Fuentealba B O Ikede F S Kibenge F Markham 《Canadian journal of veterinary research》2001,65(2):104-110
This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury. 相似文献
185.
186.
E C?té 《Veterinary Clinics of North America: Small Animal Practice》2001,31(6):1129-45, v
Severe heart disease may cause hypotension and hypoperfusion, and ultimately circulation may cease altogether. These two clinical syndromes are cardiogenic shock and cardiac arrest, respectively. This review summarizes the causes and clinical features of each, and describes the treatment options available to clinicians managing patients in cardiogenic shock. 相似文献
187.
A framework is described for partitioning the matrix of between-sample distances that forms a starting point for many ecological studies into contributions attributable to different factors in a structured data set. This partitioning enables a series of ordinations to be produced that better enable an insight to be gained into the effects of the factors. More detailed application of the same partitioning provides a decomposition of each factorial effect into single degree-of-freedom contrasts, which enables reasons for observed trends to be investigated. The methods are illustrated by application to data from a study of marine community structure. 相似文献
188.
M E Kehrli K A Weigel A E Freeman J R Thurston D H Kelley 《Veterinary immunology and immunopathology》1991,27(4):303-319
Blood neutrophil functions, lymphocyte blastogenic responses, serum complement, and serum conglutinin activity of 98 lactating Holstein cows from two genetic lines were evaluated. The genetic lines were produced in a selection experiment that created and perpetuated genetic differences in milk production for up to seven generations. No significant differences between the two genetic lines of cows were found for neutrophil function, lymphocyte blastogenic responses, serum complement levels, or serum conglutinin levels. Significant differences between sire progeny groups within lines were found for unstimulated and mitogen-stimulated lymphocyte blastogenesis (P less than 0.0001), and almost all neutrophil functions (antibody independent neutrophil cytotoxicity, antibody dependent neutrophil cytotoxicity, ingestion of bacteria, iodination, chemiluminescence, chemokinesis, and chemotaxis (P less than or equal to 0.05)). Sire progeny group differences (P less than or equal to 0.0001) within lines for serum complement and conglutinin activity were also found. Neutrophil chemiluminescence activity (positive relationship; P less than or equal to 0.001), concanavalin A-stimulated lymphocyte blastogenesis (positive relationship; P less than or equal to 0.004), and serum conglutinin activity levels (negative relationship; P less than or equal to 0.01) each had small but significant associations with the total milk somatic cell count. Cows seropositive for bovine leukosis virus had increased resting and mitogen-stimulated lymphocyte blastogenic activity and were associated with increased in vitro neutrophil random migration and production of superoxide anion. Estimates of genetic parameters of various immune cell functions, of serum complement and of conglutinin levels for daughters of 11 sires with 4-6 daughters in the data set were determined. In this report, genetic variation was demonstrated for nonspecific humoral and cellular immunity. 相似文献
189.
190.
A. E. WATERMAN A. LIVINGSTON A. AMIN 《Journal of veterinary pharmacology and therapeutics》1991,14(3):230-234
The thermal and mechanical analgesic profile of buprenorphine at a dose rate of 1.5 micrograms/kg i.v. was investigated in five sheep. This dose produced significant analgesia for 40 min against the thermal stimulus, but no mechanical antinociception. A higher dose rate of 12 micrograms/kg also failed to produce antinociception to a mechanical stimulus. In addition, the effect of the drug (6 micrograms/kg) on respiratory gas tensions was determined and no significant changes were observed. 相似文献