Biological Feasibility and Costs of Production of Large Channel Catfish,Ictalurus punctatus,for a Specialty Product Market

Restaurant patrons, particularly in upscale locations, increasingly prefer entrées developed from portions cut from fillets larger than those currently sold by the US catfish, Ictalurus punctatus, industry. A production study evaluated the feasibility of producing the necessary size (1.6 kg) of catfish. Twelve 0.1‐ha earthen ponds were stocked with 0.363‐kg channel catfish at 2500 (low); 5000 (medium); or 7500 (high) fish/ha, with four replicates. Mean individual weight at harvest exceeded the mean target weight (1.6 kg) at the two lower densities, and the minimum target weight (1.36 kg) at the highest density. Percentages of fish (by weight) that did not meet the minimum weight required were: 1, 5, and 18%, in the low, medium, and high density treatments, respectively. Yields were significantly greater at higher densities (P < 0.05). Costs of production at the two higher densities were 4 to 7% higher than in the traditional multiple‐batch system, but increased to 51% at the lowest density. Results demonstrated the biological feasibility of producing channel catfish of a mean weight of 1.6 kg. Economic feasibility depends on (1) the price premium paid and (2) development of a market for fish sizes between current acceptable maximum sizes accepted and 1.36 kg.     

Development of biofilm of <Emphasis Type="Italic">Vibrio alginolyticus</Emphasis> for oral immunostimulation of shrimp

Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation was studied at various nutrient concentrations and incubation time. Highest colony-forming units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of incubation. The BF cells could be completely inactivated at 80°C in 10 min and with 10% formalin in 24 h. SDS–PAGE profile of BF cells revealed repression of four proteins and expression of three new proteins compared to free cells (FC). The preliminary immune response studies showed that BF cells were superior to FC in stimulating the non-specific immune response of Penaeus monodon.     

In vitro storage of fish oocytes: effect of storage temperature,media conditions and storage duration on fertilization and larval hatchability of Indian major carp,rohu (Labeo rohita)

The viability of matured oocytes stored in vitro were assessed using carp ovarian fluid (OF) and artificial carp ovarian fluid (ACOF) under different temperature regimes (4, 24, 26, 28 and 30°C) for different storage durations (0, 60, 120, 150 and 180 min). Significantly higher fertilization (74%) was achieved when the oocytes were stored using ACOF and 65% in OF after 180 min at 28°C. Similarly the hatching rates of larvae were significantly higher in the ACOF and OF, that is, 64% and 47%, respectively, after 180 min of storage. The oocytes kept in the storage containers with ACOF having 65% moisture level showed a significantly higher fertilization rate than the 59% moisture level. This study demonstrated that unfertilized matured oocytes (eggs) of rohu can be stored in vitro for 180 min without compromising the viability (fertilization and hatching) to a great extent in OF and ACOF.     

The use of coal fly ash in sodic soil reclamation

An experiment was conducted for two years in northwest India to explore the feasibility of using coal fly ash for reclamation of waterlogged sodic soils and its resultant effects on plant growth in padi–wheat rotation. The initial pH, electrical conductivity, exchangeable sodium percentage and sodium adsorption ratio of the experimental soil were 9.07, 3.87 dS m⁻¹, 26.0 and 4.77 (me l)^−1/2, respectively. The fly ash obtained from electrostatic precipitators of thermal power plant had a pH of 5.89 and electrical conductivity of 0.88 dS m⁻¹. The treatments comprised of fly ash levels of 0.0, 1.5, 3.0, 4.5, 6.0 and 7.5 per cent, used alone as well as in combination with 100, 80, 60, 40, 20 and 10 per cent gypsum requirement of the soil, respectively. There was a slight reduction in soil pH while electrical conductivity of the soil decreased significantly with fly ash as measured after padi and wheat crops. The sodium adsorption ratio of the soil decreased with increasing fly ash levels, while gypsum treatments considerably added to its favourable effects. Fly ash application increased the available elemental status of N, K, Ca, Mg, S, Fe, Mn, B, Mo, Al, Pb, Ni, Co, but decreased Na, P and Zn in the soil. An application of fly ash to the soil also increased the concentrations of above elements except Na, P and Zn in the seeds and straw of padi and wheat crops. The available as well as elemental concentrations in the plants was maximum in the 0 per cent fly ash + 100 per cent gypsum requirement treatment except Na and heavy elements like Ni, Co, Cr. The treatment effects were greater in the fly ash + gypsum requirement combinations as compared to fly ash alone. Saturated hydraulic conductivity and soil water retention generally improved with the addition of fly ash while bulk density decreased. Application of fly ash up to 4.5 per cent level increased the straw and grain yield of padi and wheat crops significantly in both years. The results indicated that for reclaiming sodic soils of the southwest Punjab, gypsum could possibly be substituted up to 40 per cent of the gypsum requirement with 3.0 per cent acidic fly ash. Copyright © 2003 John Wiley & Sons, Ltd.     

Breeding high-protein pigeonpea genotypes and their agronomic and biological assessments

Proteins, inevitable for nutritional security of human beings and legumes, by far, are the cheapest source of this vital nutrient. The escalating prices and never halting population growth limit the per capita availability of protein-rich legumes. In view of limited land resource and need to grow other food crops, the greater protein harvests are possible only by increasing the protein levels of popularly grown legumes. In this context, attempts were made for raising the protein content in pigeonpea [Cajanus cajan (L.) Millsp.] through traditional plant breeding tools. For this, the high-protein trait was successfully transferred from wild relatives of pigeonpea to the cultivated types. In the derived inbred lines, the protein content was significantly enhanced from 20% - 22% to 28% - 30%. Two high-protein lines HPL 40 and HPL 8 also produced 2100 and 1660 kg/ha grain yield, respectively. This simply means that, in comparison with traditional cultivars, the cultivation of high-protein lines will provide additional 100 kg/ha of digestible protein to the farming family. This paper, besides describing the breeding procedures, also discusses the accomplishments of this breeding endeavour with respect to its various nutritional and biological properties.     

Novel quantitative trait loci for yield and yield related traits identified in Basmati rice (Oryza sativa)

Increasing crop productivity is one of the prime goals of crop breeding research. Rice grain yield is a complex quantitative trait governed by polygenes. Although several QTLs governing grain yield traits have been reported and limited attempts have been made to map QTLs for grain yield parameters in Basmati rice. A population from the cross Sonasal and Pusa Basmati 1121 comprising 352 RILs was generated through the single seed descent method. A total of 12 QTLs governing yield and yield-related traits were mapped on six chromosomes, namely, 1, 2, 3, 7, 8 and 9, of which five QTLs were novel. We identified a novel and robust epistatic QTL (qPH1.1 and qPL1.1) governing plant height and panicle length, flanked by the markers RM5336-RM1 on chromosome 1. The gene encoding brassinosteroid insensitive 1-associated receptor kinase 1 precursor is the putative candidate gene underlying this epistatic QTL. Another novel QTL, qNT3.1, governing tiller number was bracketed to a region of .77 Mb between the markers RM15247 and RM15281 on chromosome 3. Of the 57 annotated gene models, Os03g0437600 encoding alpha/beta-fold hydrolase, a homologous to AtKai2 is a putative candidate gene underlying the novel QTL qNT3.1. The other QTLs such as qDFF1.1 governing days to 50% flowering co-localizes with the gene Ghd7, QTL for plant height qPH1.2 co-localizes with the gene sd1, the QTLs for panicle length co-localizes with FUWA and DEP2, the QTL for tiller number co-localizes with OsRLCK57 and QTLs for thousand-grain weight co-localize with the major gene GS3. The QTLs identified in the current study can be effectively used in marker-assisted selection for developing Basmati rice varieties with a higher yield.     

Genetic analysis and identification of SSR marker linked to Phomopsis blight resistance in eggplant (Solanum melongena L.)

The present investigation was carried out to decipher inheritance of resistance and to identify linked SSR markers for Phomopsis blight resistance in eggplant. An F₂ population comprising 161 plants was developed from the cross of Pusa Kranti and BR-40-7. Genetic analysis was carried out using Chi square test. Artificial inoculation of fruits was carried out using pin prick method, and scoring was done as per the standard scoring scale. The F₂ plants segregated into 92 susceptible (77—highly susceptible, 15—susceptible): 69 resistant (17—highly resistant, 27—resistant, 25—moderately resistant) suggesting complimentary epistasis with ratio of 9:7. To identify the putatively linked markers to resistance gene, parental polymorphic markers were subjected to bulk segregant analysis (BSA), and two markers (emk03O04 and emf11A03) could differentiate resistant and susceptible bulk and co-segregated with resistance gene. The genetic distance between the identified markers was found to be 18.12 cM using QTL IciMapping V3.2 software depicting two new QTLs on chromosome number 6. The identified QTLs have great significant importance in marker assisted breeding programme.     

Cellulase activity in rohu fingerlings

The activity of cellulase was determined in the intestine of rohu, Labeo rohita, fingerlings fed on separate dietary formulations incorporating Leucaena leaf meal and synthetic cellulose at 20% level in proportional replacement of the ingredients from the fish-meal-based reference diet. Three more replicate experimental diets were prepared by incorporating 1% tetra-cycline, a broad-spectrum antibiotic, into the former three dietary formulations so as to circumvent the action of microflora in digestive function. Cellulase activity was found to be highest in fish fed on the cellulose incorporated diet, followed by those maintained on the plant-protein-based and reference diets, respectively. A diet-dependent variation in cellulase activity was apparent. However, a sharp decline in the level of cellulase activity was observed in the fish fed diets containing tetracycline, which is supposed to have destroyed all the gut microflora. The microbial culture of intestinal and hepatopancreatic extracts also confirmed the absence of microflora in the fish fed tetracycline-compounded diets. The study indicates that cellulase activity in rohu is largely contributed by the intestinal microflora, while the reduced activity recorded in the fish reared on antibiotic-compounded diets may be due to the presence of some other source of cellulase secretion apart from cellulolytic microbial action. The information generated from the present investigation might contribute towards better feed formulation for carp at low cost, incorporating plant-based feed ingredients. © Rapid Science Ltd. 1998     

Soil phosphorus dynamics in a Vertisol as affected by cattle manure and nitrogen fertilization in soybean‐wheat system

Repeated application of phosphorus (P) as superphosphate either alone or in conjunction with cattle manure and fertilizer N may affect the P balance and the forms and distribution of P in soil. During 7 years, we monitored 0.5 M NaHCO₃ extractable P (Olsen‐P) and determined the changes in soil inorganic P (P_i) and organic P (P_o) caused by a yearly dose of 52 kg P ha^—1 as superphosphate and different levels of cattle manure and fertilizer N application in a soybean‐wheat system on Vertisol. In general, the contents of Olsen‐P increased with conjunctive use of cattle manure. However, increasing rate of fertilizer nitrogen (N) reduced the Olsen‐P due to larger P exploitation by crops. The average amount of fertilizer P required to increase Olsen‐P by 1 mg kg^—1 was 10.5 kg ha^—1 without manure and application of 8 t manure reduced it to 8.3 kg ha^—1. Fertilizer P in excess of crop removal accumulated in labile (NaHCO₃‐P_i and P_o) and moderately labile (NaOH‐P_i and P_o) fractions linearly and manure application enhanced accumulation of P_o. The P recovered as sum of different fractions varied from 91.5 to 98.7% of total P (acid digested, P_t). Excess fertilizer P application in presence of manure led to increased levels of Olsen‐P in both topsoil and subsoil. In accordance, the recovery of P_t from the 0—15 cm layer was slightly less than the theoretical P (P added + change in soil P — P removed by crops) confirming that some of the topsoil P may have migrated to the subsoil. The P fractions were significantly correlated with apparent P balance and acted as sink for fertilizer P.