Wireworm damage to wheat seedlings: effect of temperature and wireworm state

We describe the results of four laboratory studies designed to measure the effect of temperature and wireworm appetence, weight, and degree of Metarhizium infection on their ability to damage wheat seedlings. Wireworm activity, measured from wireworm speed, increased linearly from 6 to 18 °C and leveled off thereafter. Plant emergence and growth increased exponentially from 6 to 22 °C for wheat cultivars AC Barrie and AC Unity VB. Plant root:shoot ratio at Zadoks 13 was highest at 14 °C and lowest at 22 °C for AC Barrie. Wireworm weight and degree of infection with Metarhizium did not affect their ability to kill wheat seedlings, but wireworms in a feeding state caused significantly more damage than those in a non-feeding state when wheat was grown at 10, 14, 18, and 22 °C. Wireworms (ww) in a feeding state destroyed 1.8 seedlings/ww in 14 days at 22 °C if there were 1 or 2 wireworms in a pot, and 1.5 seedlings/ww if there were 4 wireworms in a pot. If 5 wireworms were placed in a pot, wireworms in a feeding state destroyed 0.3, 1.0, 0.9, 1.3, and 1.4 seedlings/ww in 46, 32, 25, 25, and 25 days at 6, 10, 14, 18, and 22 °C, respectively. Wireworm mortality from Metarhizium during 60 days of containment in pots in the study was higher in non-feeding than in feeding wireworms, and higher if wireworms were selected from a Metarhizium-infected colony than those selected from a non-infected colony. Some of the implications of these results for wireworm management and laboratory trials are discussed.     

Interspecies comparison of morphology, ultrastructure, and proteome of mammalian keratin fibers of similar diameter

Sheep wool has traditionally been viewed as the representative mammalian keratin fiber for the purposes of describing morphology and protein composition. We have investigated narrow fibers from the under-hairs of a range of species both closely and distantly related to sheep, comparing structure and protein composition. Within this group, curvature was negatively correlated with diameter for all but mohair. The cortical cell types present in alpaca, rabbit, and mohair fibers differed structurally from wool, primarily in terms of their macrofibril architecture. Except for rabbit, each species' fibers contained three cell types, and except for mohair, cell types were distributed asymmetrically across the cortex. In mohair, the cell types were distributed annularly, and each cell type had regions in which intermediate filaments were packed into highly aligned hexagonal mosaics, much like the mesocortex in wool. Coupled with this, were differences in the protein profiles; the rabbit fiber contained extra keratins and keratin associated proteins, while only subtle differences were noted between mohair and Merino fibers. In both rabbit and mohair fibers, the relative abundance of keratin K85 was lower than that of Merino. These results suggest that there may be links between relative protein composition and fiber morphology, albeit complex ones.     

Aspects of protein metabolism and its controlAspects de la régulation du métabolisme des protéinesAspekte des Proteinstoffwechsels und seiner Kontrolle

Protein metabolism is a highly integrated process. The rates of deposition in a tissue depend on the balance between synthesis and degradation. While a lot is known about the factors controlling synthesis, e.g. amino acid supply, availability of messenger RNA, much less is known about the factors controlling degradation. Both these processes are markedly affected by hormones. When considering protein metabolism in the context of attempting to determine the factors controlling growth, it is essential to consider its integration between organs.     

Biliary secretion of 14C and identification of 5,6-dihydro-5,6-dihydroxycarbaryl glucuronide as a biliary metabolite of [14C]carbaryl in the rat

The biliary secretion of ¹⁴C was observed in conscious, bile-fistulated rats given single oral doses of [¹⁴C]carbaryl (1.5, 30, and 300 mg/kg). Over 94% of the ¹⁴C was absorbed after 12 hr. From 15 to 46% of the ¹⁴C was secreted in bile, 10–40% in urine, and less than 1% in feces 12 hr after dosing. Three metabolites were isolated from bile and identified by mass and/or NMR spectrometric methods. These metabolites were: 5,6-dihydro-5,6-dihydroxycarbaryl glucuronide (12–18% of the biliary ¹⁴C), a conjugate(s) of carbaryl (12% of the biliary ¹⁴C), and conjugated isomers of hydroxy-carbaryl (2% of the biliary ¹⁴C). The majority of the biliary ¹⁴C remains to be identified.     

Protein metabolism in animals treated with anabolic agents

One of the major factors controlling the deposition of protein in an animal is the activity of the hormones circulating in its blood. Many of the anabolic hormones interact with each other e.g. growth hormone and insulin and there is evidence for a direct interaction between catabolic and anabolic hormones e.g. testosterone and glucocorticoids. Exogenously administered hormone-like substances can have marked effects on animal growth. Diethyl stilbestrol acts like an oestrogen elevating plasma insulin and growth hormone concentration. Zeranol probably also acts in a similar way.Trenbolone acetate (TBA) an androgenic agent, is a very effective growth promotor especially in ruminants. Few changes of note in the endogenous plasma hormone concentrations in treated ruminants have been reported although the combined implant of TBA plus 17-oestradiol did depress plasma thyroxine in steers. We have used the rat as a model to test the effects of TBA on protein synthesis and protein degradation rate in the muscle of rats. Protein synthesis and protein degradation in the muscle of treated female rats was shown to be markedly reduced, the increased growth rate being brought about by a greater reduction in the rate of degradation than in the rate of synthesis. Cathepsin D activity in the muscle was also reduced. Attempts to demonstrate a direct action of TBA on muscle have been unsuccessful. The currently favoured hypothesis for the mode of action of TBA is that it interferes with catabolic action of glucocorticoids on muscle protein. This may not be the mode of action of all androgenic agents. Durabolin (nandrolone phenyl propionate) would appear to stimulate both protein synthesis and protein degradation, at least in the rat.     

Discourses of Elk Hunting and Grizzly Bear Incidents in Grand Teton National Park,Wyoming

Incidents of human–wildlife conflict can solidify participant perspectives and expectations, and reveal underlying social and institutional dynamics. We describe and analyze two incidents of conflict between hunters and grizzly bears in 2011 and 2012. Both incidents were associated with the controversial elk hunt in Grand Teton National Park, Wyoming. We gathered quotes from opinion-editorials and interviews, categorized quotes according to type, and used cluster analysis to identify discourses and associated coalitions of participants. We examined how participants defined problems, used evidence, and advocated solutions in relation to these incidents. Participant discourses addressed technical and procedural issues of regional wildlife management that extended beyond these incidents, revealing divergent expectations about wildlife management on public lands. Existing decision-making processes dominated by government agencies do not appear to be addressing these expectations. We recommend an integrative, outside appraisal of regional elk management to address conflict and support ecologically sound decision-making that serves common interests.     

Genetic control of dry matter,starch and sugar content in sweetpotato

Sweetpotato (Ipomoea batatas L. (Lam)) is a nutritious food security crop for most tropical households, but its utilisation is very low in Ghana compared to the other root and tuber crops due to lack of end-user-preferred cultivars. Knowledge on the genetic control of important traits such as dry matter, starch and sugar content of sweetpotato storage roots in a breeding population is critical for making breeding progress in developing sweetpotato varieties preferred by farmers and consumers. This study used diallel mating design to elucidate general combining ability and specific combining ability, to determine the gene action controlling storage root dry matter, starch and sugar content in sweetpotato and the heterotic potential of the traits to facilitate the crop’s improvement for increased utilisation. A general model for estimating genetic effects, GEAN II, was used to analyse the data. Genetic variability was seen for dry matter, starch and sugar content of sweetpotato and much of this genetic variation was additive in nature. The study also revealed significant heterosis in sweetpotato which offers opportunity for breeding non-sweet, high dry matter sweetpotato varieties that are preferred by farmers and consumers in Ghana.     

Combining ability and heterosis of elite drought-tolerant maize inbred lines evaluated in diverse environments of lowland tropics

Estimates of combining abilities and heterosis of inbred lines are imperative for selection of suitable parents of maize hybrids. This study examined the combining ability of 24 drought-tolerant maize inbred lines, 12 each from International Centre for Maize and Wheat Improvement (CIMMYT) and International Institute of Tropical Agriculture (IITA). The lines were allotted into six groups each comprising four lines. The four lines in one group were used as females and crossed to the four lines in another group as males in six different sets using a North Carolina Design II mating scheme to generate 96 hybrids. The hybrids were evaluated together with four checks across six environments in the rainforest and savannah agro-ecologies of Nigeria between 2011 and 2012. The parental inbred lines were also evaluated in separate trial in each location. Significant hybrids × environment interaction was observed for grain yield and other measured traits. GCA effects accounted for 83.3% of the variation for grain yield at Bagauda, 78.1% at Saminaka, and 77.7% at Ikenne. GCA also contributed 91.1 and 80.0% to the variation observed for plant height and ear aspect, respectively, across the environments. Significant SCA × environment interaction detected for grain yield suggests that hybrids were not stable across test environments. Prediction of grain yield in hybrids using midparent values resulted in a R ² value of 0.13. Midparent heterosis for grain yield varied from 80 to 411%, with the top 36 hybrids recording >200%. Four CIMMYT (EXL02, EXL06, EXL04 and EXL16) and three IITA (ADL33, ADL41, and ADL32) inbred lines had positive and significant GCA effects for grain yield across environments. The novel alleles present in the CIMMYT lines will improve the adapted IITA germplasm in a new population for extracting new set of more productive inbred lines for developing adapted high yielding drought-tolerant maize hybrids.