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Greci V Travetti O Di Giancamillo M Lombardo R Giudice C Banco B Mortellaro CM 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(6):631-636
Middle ear cholesteatoma is a rare condition in dogs with chronic otitis. Otorrhea, otodinia, and pain on temporomandibular joint palpation are the most common clinical signs. Neurological abnormalities are often detectable. Computed tomography reveals the presence of an expansive and invasive unvascularized lesion involving the tympanic cavity and the bulla, with little or no contrast enhancement after administration of contrast mediu. Video-otoscopy may detect pearly growth or white/yellowish scales in the middle ear cavity. Surgery is the only therapy but is associated with a high risk of recurrence. 相似文献
104.
Terpenic acids are under development as therapeutic agents in numerous treatments. In support of pharmacokinetic and toxicological evaluations, a robust assay based on high-performance liquid chromatography (HPLC) was developed for the analysis of the terpenoids in human serum. For a clear understanding of the differences in biological activity of these compounds, the interactions between oleanolic or betulinic acids and human serum protein have been studied by ultraviolet-visible (UV-vis) absorption under physiological conditions. A combination of liquid/liquid extraction, centrifugation, and consecutive HPLC resulted in simultaneous separation, identification, and quantification of the oleanolic, betulinic, and ursolic acids. The validity of the developed method was established by determining linearity, recovery, precision, accuracy, limit of detection, and quantification. Detection limits were in the range of 3.3-4.3 ng/mL, and linearity values ranged up to 1 μg/mL. The repeatability of the method was good. All compounds can be well-distinguished by order of elution during liquid chromatography. The pentacyclic triterpenoids have been identified by retention time comparison to pure standards and quantified by an internal standard. The results by UV-vis absorption spectra experiments (240-340 nm) indicate that protein structures have been perturbed in the presence of oleanolic and betulinic acids. 相似文献
105.
The changes in chlorophyll a fluorescence, caused by high temperature (HT), have been used to study heat-tolerance in nine common bean (Phaseolus vulgaris L.) accessions (3 cultivars and 6 lines) available in the gene banks of Maritsa Vegetable Crops Research Institute and Institute for Plant Genetic Resources. The plants were grown in controlled greenhouse conditions until the blossoming stage, treated with HT (45° C) for 2 h and returned for recovery for 4 h at 23° C. The obtained results allowed us to differentiate four groups, among the studied bean plants, by their response to HT. The first group, includes a single cultivar – ‘Secuntsa’. The accession was considered heat sensitive, because it showed a decrease of its total performance index (PItotal), calculated by JIP-test, during HT stress and did not recover to the initial values. The second group, comprised of lines RH13, BBSR17, BBSR28, and ‘Starozagorski cher’, expressed an increase in PItotal during heat stress, which however was followed by a decline in PItotal values during recovery after HT treatment. The third group – RRR46 displayed a decrease in various JIP-test parameters during HT treatment followed by full recovery after returning the plants to 23° C. The accessions from the fourth group RH26D, ‘Ranit’, similarly to the control heat tolerant line 83201007 did not show significant alteration in PItotal. We assumed that the accessions from the fourth group are heat-tolerant. The line RRR46 is also promising in terms of heat tolerance because of its flexible response to HT treatment. These genotypes will be used in our further breeding program. 相似文献
106.
Eva-Maria Halbauer Valentina Bohinec Melanie Wittenberger Karin Hansel-Hohl Stephan Gaubitzer Eva M. Sehr 《Euphytica》2017,213(6):120
Agro-biodiversity is currently experiencing severe genetic erosion due to mankind’s unsustainable activities. Because of initiatives following the goal of the conservation of biological diversity, so far seven million crop accessions are being conserved ex situ in gene banks worldwide. Many of these accessions are landraces being rich in gene diversity, silently awaiting their proper characterisation. This is a very critical part of any long-term strategy to enhance the productivity and resilience of crops and agricultural systems and—most importantly—to ensure the preservation of our cultural and biological heritage. In this study of an ex situ germplasm evaluation we analysed 27 flax (Linum usitatissimum L.) accessions originating in the Alpine region, provided by five local gene banks/providers. Based on genomic microsatellite markers (gSSRs), a varying extent of accession-specific gene diversity (expected heterozygosity, HE) was revealed ranging from 0.05 to 0.51. Admixture of individuals between accessions was uncovered, pointing towards past processes related to gene bank management activities (e.g. intentional selection, unintentional cross-pollination during regeneration) or towards the evolution of the landrace itself (e.g. same regional origin, traditional naming), highlighting the co-existence of cultural and biological diversity. Such an genetic analysis of accessions stored ex situ not only produces valuable agronomic and breeding data, but also is useful for the clarification of past processes leading to duplicates within and between collections or mislabelling, contributing to the potential for rationalisation of collections, which in turn can help ensure that the limited resources available for regeneration are used most efficiently and effectively. 相似文献
107.
The present review aims to provide insight into the complex interactions between the host and Pseudomonas aeruginosa—an opportunistic microbial agent causing skin infections. Heat, humidity and skin pH are among the factors beneficial for the development of this Gram-negative agent. To cause infection, Pseudomonas aeruginosa should first overcome the primary mechanisms of defense including the cell elements and humoral factors of the skin, as well as non-specific responses—phagocytosis, inflammation, acute phase response. All they are analysed with emphasis on the fact that their detailed understanding would help revealing their potential and allow for their efficient control. 相似文献
108.
Bagardi Mara Palermo Valentina Locatelli Chiara Colombo Fabio Maria Pazzagli Laura Brambilla Paola G. 《Veterinary research communications》2022,46(2):481-486
Veterinary Research Communications - The aim of this work was to retrospectively evaluate the influence of pimobendan on the survival time (ST) of dogs with myxomatous mitral valve disease at... 相似文献
109.
Andrea Mencarelli Claudio D’Amore Barbara Renga Sabrina Cipriani Adriana Carino Valentina Sepe Elisa Perissutti Maria Valeria D’Auria Angela Zampella Eleonora Distrutti Stefano Fiorucci 《Marine drugs》2014,12(1):36-53
In the present study we provide evidence that solomonsterol A, a selective pregnane X receptor (PXR) agonist isolated from the marine sponge Theonella swinhoei, exerts anti-inflammatory activity and attenuates systemic inflammation and immune dysfunction in a mouse model of rheumatoid arthritis. Solomonsterol A was effective in protecting against the development of arthritis induced by injecting transgenic mice harboring a humanized PXR, with anti-collagen antibodies (CAIA) with beneficial effects on joint histopathology and local inflammatory response reducing the expression of inflammatory markers (TNFα, IFNγ and IL-17 and chemokines MIP1α and RANTES) in draining lymph nodes. Solomonsterol A rescued mice from systemic inflammation were assessed by measuring arthritis score, CRP and cytokines in the blood. In summary, the present study provides a molecular basis for the regulation of systemic local and systemic immunity by PXR agonists. 相似文献
110.
Tuberoso CI Kowalczyk A Coroneo V Russo MT Dessì S Cabras P 《Journal of agricultural and food chemistry》2005,53(26):10148-10153
The chemical composition of the essential oil from flowering tops of Achillea ligustica All. was studied. Samples were collected in different localities of Sardinia (Italy) and hydrodistilled both with Clevenger-type and with simultaneous distillation-extraction apparatus. The yields ranged between 0.88 +/- 0.06 and 0.43 +/- 0.02% (vol/dry wt). The essential oils were analyzed by GC-MS, and a total of 96 components were detected. From a qualitative point of view, irrelevant differences between samples were observed. Strong chemical variability depending on the origin of the samples was observed. The major compounds found were santolina alcohol (6.7-21.8%, for the first time detected in A. ligustica), borneol (3.4-20.8%), sabinol (2.1-15.5%), trans-sabinyl acetate (0.9-17.6%), alpha-thujone (0.4-25.8%), and, among sesquiterpenes, viridiflorol (0.7-3.6%). No significant differences were detected between essential oils extracted by hydrodistillation and simultaneous distillation-extraction with CH2Cl2 and n-hexane. Antioxidant activity as DPPH radical scavenging activity was expressed in TEAC and ranged between 0.40 and 0.88 mmol/L. The antimicrobial and antifungal activities were investigated on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Penicillium commune, Fusarium oxysporum, Rizoctonia solani, and Aspergillus flavus, showing low activity. 相似文献