羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

吉林省东部半山区“四密25”玉米产量构成因素的浅析

四密25是优质高产适应性较强的玉米品种,虽创造出吉林省东部半山区15000kg/hm2的高产记录,但产量波动(7500～15000kg/hm2)较大。为进一步研究其高产稳产的构成因素,1994-2004年,在吉林省东部半山区桦甸市5个乡镇,应用四密25分别进行了品种比较、高产攻关、覆膜种植与裸地种植、不同施肥量、施肥方法、播种方法、播种时期、不同密度及等离子体处理种子的剂量、次数、播种期、化肥利用率的试验。通过对上述各项试验的194份材料统计分析,明确了四密25构成产量模型的3个主导因素为千粒重、穗粒数、公顷穗数。根据回归分析理论,计算出产量与3个主导因素之间的模型,为今后吉林省东部半山区四密25高产稳产提供理论依据。     

支付交易费的回望期权定价

以Black-Scholes模型为基础,通过对回望期权的研究,结合有交易费的欧式期权的定价公式,运用证券组合技术与无套利原理,建立了支付交易费的回望期权定价模型.通过对方程化简和分析,运到PDE相关方法化为Cauchy问题,得出定价公式.     

5株野鸭源H6N1亚型禽流感病毒NA基因的克隆和进化分析

根据GenBank已知的H6N1亚型流感病毒的NA基因序列,设计扩增NA基因的特异性引物,通过RTPCR技术扩增5株H6N1亚型禽流感病毒NA基因序列,并将其克隆到pMD18-T载体后进行了测序分析.同时通过生物学软件对5株禽流感病毒NA基因进行了分析研究.结果表明:5株禽流感病毒NA基因全长1 410 bp,编码47...     

微射流均质预处理提高大豆分离蛋白酶解效率及酶解产物乳化性能

为了探讨微射流均质预处理对大豆分离蛋白酶解效率及酶解产物乳化性能的影响,该文研究比较了微射流均质预处理前后大豆分离蛋白酶解产物的理化性质(水解度、亚基组成、蛋白溶解性、表面疏水性和分子量分布)和乳化性能(通过测定分析样品乳状液的平均粒径和微观结构评估样品的乳化性能)的变化。研究表明:大豆分离蛋白经过微射流均质预处理后采用木瓜蛋白酶水解,其酶解产物(水解度为1.7%)与对照大豆分离蛋白和未经预处理的酶解产物相比,在较低浓度下(30 g/L)制备出粒径细小的稳定乳状液(体积平均粒径≈1.6μm)。微射流均质预处理提高了大豆分离蛋白中α-7S和A-11S亚基的酶解敏感性,使酶解产物在水解度1.3%~1.7%范围内蛋白溶解性显著增加(P0.05),同时保持较高的表面疏水性值,与未经预处理的酶解产物相比形成了更多具有界面活性的可溶性多肽(分子量主要分布在11.3 k Da左右),在乳化过程中可有效防止乳液滴间发生桥联絮凝。因此微射流均质预处理是一种辅助提高大豆蛋白酶解效率和酶解产物乳化性能行之有效的方法。研究结果可为大豆蛋白深加工蛋白乳化剂提供理论和方法参考。     

Simultaneous immobilization of lead,cadmium, and arsenic in combined contaminated soil with iron hydroxyl phosphate

Purpose

Combined contamination of lead (Pb), cadmium (Cd), and arsenic (As) in soils especially wastewater-irrigated soil causes environmental concern. The aim of this study is to develop a soil amendment for simultaneous immobilization of Pb, Cd, and As in combinative contaminated soil.

Materials and methods

A soil amendment of iron hydroxyl phosphate (FeHP) was prepared and characterized, and its potential application in simultaneous immobilization of Pb, Cd, and As in combined contaminated soil from wastewater-irrigated area was evaluated. The effects of FeHP dosage, reaction time, and soil moisture on Pb, Cd, and As immobilization in the soil were examined.

Results and discussion

The immobilization efficiencies of Pb, Cd, and As generally increased with the increasing of FeHP dosage. With FeHP dosage of 10 %, the immobilization percentages of NaHCO₃-extractable As and DTPA-extractable Pb and Cd reached 69, 59, and 44 %, respectively. The equilibrium time required for immobilization of these contaminants was in the following order: NaHCO₃-extractable As (0.25 days) < DTPA-extractable Cd(3 days) < DTPA-extractable Pb (7 days). However, the immobilization efficiencies of Pb, Cd, and As have not changed much under soil moisture varied from 20 to 100 %. According to the results of the sequential extraction, the percentages of Pb, Cd, and As in residual fractions increased after the application of FeHP amendment, while their percentages in exchangeable fractions decreased, illustrating that FeHP can effectively decrease the mobilities and bioavailabilities of Pb, Cd, and As in the soil. Moreover, the application of FeHP will not have soil acidification and soil structure problem based on the soil pH measurements and soil morphology.

Conclusions

FeHP can immobilize Pb, Cd, and As in the combinative contaminated soil from wastewater irrigation area simultaneously and effectively. Thus, it can be used as a potential soil amendment for the remediation of Pb, Cd, and As-combined contaminated soil.

     

手持式电动绿篱修剪机的研究与设计

通过对目前市场上绿篱修剪机结构和使用特点的详细分析,设计了一种用220V照明电为电源、手提砂轮机作为主机,用专门制作的切割器替换砂轮片实现快速修剪成形的手持式电动绿篱修剪机。试验表明,该修剪机有很好的实用价值和推广前景。     

Development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of Mycoplasma suis antibodies

The recombinant ppa protein of Mycoplasma suis migrated to 21 kDa. Using this antigen, an ELISA system to detect the antibody against M. suis infection in swine was established. The rELISA demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with M. suis infection. A comparison of this ELISA system with an indirect hemagglutination assay (IHA) test using 132 swine samples revealed that the positive rate was 34.0% in ELISA and 28.0% in IHA. Compared with IHA, the present rELISA system using recombinant ppa antigen significantly improves the specificity, sensitivity, and stability for serodiagnosis of M. suis infection in swine.     

黄土高原重力侵蚀的地形与岩性组合因子分析

黄土高原地区严重的重力侵蚀,是该区的主要侵蚀方式之一。本文运用计量地貌学方法,对该区重力侵蚀的地形因子进行了分析,提出了重力侵蚀的地形因子值概念,编制了全区区域临空面高度以及地形因子等值线图,给出了不同岩性组合条件的重力浸蚀强度地形因子指标。最后还探讨了重力侵蚀强度的区域特征。     

喷头射程理论公式与试验研究

在无风有空气阻力的假定条件下,根据牛顿第二运动定律和水力学基本理论,推导出喷头射程的理论公式,并选取典型微喷头和喷头,在室内进行喷头射程影响因素的试验。试验结果表明：与国内外常用的经验公式相比,理论公式的计算值与实测值吻合较好,相对误差仅为5.05%。理论公式物理意义明确,计算精度高,适用于各种微喷头和喷头。