Intramammary infusion of an Enterococcus faecium SF68 preparation promoted the involution of drying off Holstein cows partly related to neutrophil‐associated matrix metalloproteinase 9

A problem for dairy cows following milk stasis is to cope with a high risk of intramammary infection and there is a need to initiate an extensive renewal of secretory modules in mammary glands so that milk production in next lactation may be optimized. We recently reported that ultrasonicated Enterococcus faecium SF68 (SF68) is compatible with cow mammary glands and an enhancer of innate immunity during the immediate post‐milk stasis period. The current study further examines the concomitant effect of ultrasonicated SF68 on mammary tissue remodeling. Four Holstein cows each received intramammary infusions of regular antibiotic dry‐cow formula (positive control) and two different doses of SF68 in different quarters. Analyses of individual quarter secretion samples showed faster neutrophil infiltration, earlier modifications in protein composition, including caseins and lactoferrins, as well as more prompt elevation of the specific unit of 92‐kDa matrix metalloproteinase 9 (MMP9) in SF68‐infused quarters compared to the positive controls. Intramammary infusion of ultrasonicated SF68 seems able to accelerate the regression of mammary synthetic capacity and potentiate the breakdown of glandular extracellular matrix, indicating a more efficient mammary gland involution. Correlation analyses imply that the ability of ultrasonicated SF68 to induce faster neutrophil chemotaxis and the associated MMP9 release is partly responsible.     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

欧洲动物生产与营养研究的现状和发展趋势

本作在欧洲从事博士后研究期间,几次参加相关学术讨论会,并接触到一些反映最新发展趋势的资料和信息.在此基础上,结合作本人对相关问题的思考,撰写了本篇章.本从21世纪动物生产面临的新问题、新挑战出发，论述了动物生产的新进展和新要求，动物营养研究应用的新技术和新方法，目前动物生产与营养研究的领域和热点问题等，概略论述了目前欧洲动物生产与营养研究的现状和发展趋势，供有关人员参考。     

Multi‐channel visual evoked potential as an ancillary tool to diagnose intraorbital optic nerve lesions

Multi‐channel visual evoked potential (MVEP) recording method was used to assist in diagnosing a 4‐month‐old Chinese Albino rabbit with an intraorbital mass. Subcutaneous MVEP of its both eyes were recorded simultaneously using 16 electrodes (4 × 4) multi‐channel array. Analysis of the cortical potential landscapes (CPL) showed that the conduction function of right eye was remarkably impaired in terms of decreased amplitudes and prolonged latencies. Specific side‐dominant distribution asymmetry of the decreased MVEP amplitudes indicated that the temporal side of the optic nerve (ON) was severely involved. Overall prolonged latencies of the CPL without side differences suggested that the functional impairment could have been caused by the mechanical compression exerted by an intraorbital mass. Surgical removal procedures confirmed that the mass was located temporally to the ON. Pathological examination provided a final diagnosis of a giant polycystic mucocele. Beyond its significance as a standard tool to assess functional changes of the visual pathway, MVEP recordings might assist locating intraorbital lesions that involve the ON by careful analysis of abnormal CPLs.     

确定潜水含水层系统参数的Boulton-RAGA方法

在对考虑迟后排水的N.S.Boulton第二潜水井流模型解析解分析的基础上，将复合高斯求积法和具有随机搜索寻优特性的实数编码加速遗传算法(RAGA)相结合对其进行优化求解，提出了确定潜水含水层系统参数的Boulton-RAGA法。以计算实例表明，Boulton-RAGA法可以取得很好的求参效果，并且与传统方法比较，方法简单，快速，不需要分抽水时间——降深过程的前、后段进行参数确定，大大简化了潜水含水层的参数确定过程，对于缩短抽水时间确定含水层系统参数具有重要的意义。     

视频和GIS技术在森林防火中的应用

针对浙江省森林覆盖率高,并且发生森林火灾的频率也高,单纯的视频监控在夜晚无法识别等问题,提出了视频技术与地理信息系统技术相结合来监控森林火灾的方案。该方案是在森林的某个高程点上架设视频摄像设备,通过远程传输到达视频监控系统的后台,显示在森林地理信息系统上,然后根据显示的区域来监控森林火情。该方案通过同步跟踪算法实现视频技术与地理信息系统的融合,在森林防火地理信息系统上实时同步跟踪,能够提高森林防火的效率。     

略议喷灌技术规范

1985年，水利部曾经组织不少单位，编制了一整套《喷灌工程技术规范》及所有相关附件资料，国家标准编号CBJ85－85，这套文件在10余年中一直是中国喷灌事业的圭臬，对其发展壮大起了很重要的指导作用，其中的许多部分，对今后喷灌事业仍将有着十分重要的意义。但是，毕竟时代已经进入到了1998年，与10多年以前的1985年相比较，中国各方面都产生了重大的变化，在新的情况下，原有规范似有修改增订的必要。众所周知，许多发展国家，它的标准、规范等方面的资料，一般少则2－3年，多则7－8年，基本上都要重新修订，以适应社会进步需要，中国…     

长三角地区某市垃圾焚烧行业环保现状调查研究

对长三角地区某市垃圾焚烧行业的环保现状进行了调查研究,结果发现:当前区域垃圾焚烧企业,在保证规模、控制数量及地域上均衡布置的前提下,行业废水、废气污染治理设施齐全,废水、废气均稳定可达标排放,调查时段内未有投诉问题。区域垃圾焚烧行业在做到当下要求的情况下,实现了区域垃圾焚烧行业污染可控,对城市垃圾焚烧行业环保发展具备借鉴意义。     

贵州长坡岭林场马尾松毛虫工程治理技术设计

森林病虫害工程治理是一种新的病虫害管理方式，技术设计应严格按工程治理有关规定进行，要体现依靠科学技术和“预防为主，综合防治”的原则，明确治理目标、任务和效果，注意长、中、短期措施相结合，并将各项工程治理措施落实到各实施年度、小班。     

THE CHARACTERS AND EFFECTS OF AVAILABLE PHOSPHORUS IN DAHURIAN LARCH-MANCHURICA ASH MIXED SEEDLING STAND

Dahurianlarch(Larixgmelinii)isamaintreesPeciesinartificialstandsandManchuriaash(Fnainusma:dehurica)isavaluabIehardwoodsPeciesinti1eforestareaofnortheastChina.SomestUdiesrevealedthatthemixedstandbythetwospecieshadpositivemixedeffectSandnutritionrelationshipwasoneofthemaincausesforthepositiveeffects.lnsomeresearches,itwasfoundthatinline-belttwoflarch-ashmixedstand,theconcentra-tionofavailablephosphorusofsoilinlarchbeltwas2ntimesoftl1atinashbelt.Itisdeducedthatinsuchmixedstandtheashcanabsorbmo…