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81.
82.
It recently has been reported that the non-plant-pathogenic oomycete Pythium oligandrum suppresses bacterial wilt caused by Ralstonia solanacearum in tomato. As one approach to determine disease-suppressive mechanisms of action, we analyzed the colonization of P. oligandrum in rhizospheres of tomato using real-time polymerase chain reaction (PCR) and confocal laser-scanning microscopy. The real-time PCR specifically quantified P. oligandrum in the tomato rhizosphere that is reliable over a range of 0.1 pg to 1 ng of P. oligandrum DNA from 25 mg dry weight of soil. Rhizosphere populations of P. oligandrum from tomato grown for 3 weeks in both unsterilized and sterilized field soils similarly increased with the initial application of at least 5 x 10(5) oospores per plant. Confocal microscopic observation also showed that hyphal development was frequent on the root surface and some hyphae penetrated into root epidermis. However, rhizosphere population dynamics after transplanting into sterilized soil showed that the P. oligandrum population decreased with time after transplanting, particularly at the root tips, indicating that this biocontrol fungus is rhizosphere competent but does not actively spread along roots. Protection over the long term from root-infecting pathogens does not seem to involve direct competition. However, sparse rhizosphere colonization of P. oligandrum reduced the bacterial wilt as well as more extensive colonization, which did not reduce the rhizosphere population of R. solanacearum. These results suggest that competition for infection sites and nutrients in rhizosphere is not the primary biocontrol mechanism of bacterial wilt by P. oligandrum. 相似文献
83.
Improved porcine model for Shiga toxin‐producing Escherichia coli infection by deprivation of colostrum feeding in newborn piglets
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Toshio Sato Takashi Hamabata Eiji Takita Takeshi Matsui Kazutoshi Sawada Taishi Imaoka Nobuo Nakanishi Keizo Nakayama Takamitsu Tsukahara 《Animal Science Journal》2017,88(5):826-831
Porcine edema disease (ED) is a toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)‐producing Escherichia coli (STEC). ED occurs most frequently during the weaning period and is manifested as emaciation associated with high mortality. In our experimental infection with a specific STEC strain, we failed to cause the suppression of weight gain in piglets, which is a typical symptom of ED, in two consecutive experiments. Therefore, we examined the effects of deprivation of colostrum on the sensitivity of newborn piglets to STEC infection. Neonatal pigs were categorized into two groups: one fed artificial milk instead of colostrum in the first 24 h after birth and then returned to the care of their mother, the other breastfed by a surrogate mother until weaning. The oral challenge with 1011 colony‐forming units of virulent STEC strain on days 25, 26 and 27 caused suppression of weight gain and other ED symptoms in both groups, suggesting that colostrum deprivation from piglets was effective in enhancing susceptibility to STEC. Two successive STEC infection experiments using colostrum‐deprived piglets reproduced this result, leading us to conclude that this improved ED piglet model is more sensitive to STEC infection than the previously established models. 相似文献
84.
Canopy temperature on clear and cloudy days can be used to estimate varietal differences in stomatal conductance in rice 总被引:1,自引:0,他引:1
Leaf photosynthetic potential, a major determinant of yield potential in rice, is mostly explained by stomatal conductance (gs) as well as leaf N content (Rubisco protein content). Therefore, the rapid and precise evaluation of gs as well as leaf N content would be a valuable addition to rice breeding programs aimed at improved yield potential. We established a simple method based on infrared thermography to estimate varietal differences in gs. Seven rice varieties were cultivated in two-row plots in 2007, and canopy temperatures in three varieties, including a control variety, Koshihikari, were simultaneously measured to determine canopy temperature difference (CTd) between Koshihikari and the other varieties on clear days. The varieties Takanari, Habataki, and TUAT1-5-6a displayed significantly (P < 0.05) lower leaf temperature and higher gs and leaf photosynthetic rate (Pn) than Koshihikari. CTd was closely correlated with gs and Pn. These results indicate that CTd may be useful as a relative index to estimate varietal differences in gs. A significant CTd was still observed even under cloudy conditions in 2008. Takanari and TUAT1-5-6a had lower leaf temperature than Koshihikari on cloudy days as well as on clear days. Subsequent investigation of photosynthetic light response curves revealed that Takanari and TUAT1-5-6a had higher gs and Pn than Koshihikari under lower irradiance. These results suggest that infrared thermography may be a simple method of evaluating varietal differences in gs through CTd, and that it may be practicable even under cloudy conditions. 相似文献
85.
Asai T Namimatsu T Osumi T Kojima A Harada K Aoki H Sameshima T Takahashi T 《Comparative immunology, microbiology and infectious diseases》2010,33(2):109-119
Salmonella enterica serovar Choleraesuis (S. Choleraesuis) isolates derived from diseased pigs in Japan during 2001 and 2005 were analyzed for biotype, based on H(2)S production and dulcitol fermentation, pulsed-field gel electrophoresis (PFGE) profile, and antimicrobial resistance profile. S. Choleraesuis biotype Choleraesuis (biotype Choleraesuis) was classified into one genotype, while varietas Kunzendorf (var. Kunzendorf) was classified into two genotypes. The isolates of var. Kunzendorf belonging to one genotype were isolated in a limited area of Japan. Variation in the antimicrobial resistance pattern was observed in isolates of both biotypes Choleraesuis and var. Kunzendorf. We have also shown that the PFGE profile was associated with the biotype and isolation region of each isolate. 相似文献
86.
Kubo K Entani T Takara A Wang N Fields AM Hua Z Toyoda M Kawashima S Ando T Isogai A Kao TH Takayama S 《Science (New York, N.Y.)》2010,330(6005):796-799
Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants. 相似文献
87.
88.
Improvement of paddy field drainage for mechanization 总被引:1,自引:1,他引:1
In Japan, land consolidation and drainage improvement for farm mechanization in paddy fields began during the 1960 s. It was not easy to use big machines in the muddy conditions caused by the clayey soil and heavy rainfall during the harvesting period. A number of investigations were carried out by many researchers, and factors relating to drainage were clarified. Not only surface drainage but also underdrainage was planned. However, drainage was not always sufficient, because the clayey surface soil was impermeable to ponding water. It became clear that underdrainage for a clayey paddy field for the harvest is quite different from underdrainage for an ordinary field. Field and soil characteristics, as well as water conditions, should be examined carefully before planning drainage improvement for farm mechanization.Dr. Toshio Tabuchi is an honorable member of the Japanese Society of Irrigation, Drainage and Reclamation Engineering (JSIDRE), a former Professor of the University of Tokyo, and a former President of JSIDRE 相似文献
89.
Norikura T Fujiwara K Narita T Yamaguchi S Morinaga Y Iwai K Matsue H 《Journal of agricultural and food chemistry》2011,59(13):6974-6979
Thelephora aurantiotincta is an edible mushroom belonging to the genus Thelephora; it grows in symbiosis with pine trees. Recently, phytochemical investigations have revealed that the genus Thelephora is an abundant source of p-terphenyl derivatives. However, their bioactivity has not yet been well characterized. In screening for natural materials with anticancer activity, a T. aurantiotincta ethanol extract (TAE) was found to decrease cell viability in human hepatocellular carcinoma cells (HepG2). In this study, a new p-terphenyl derivative, thelephantin O, and a known compound, vialinin A, were isolated as the principal bioactive components of TAE. These compounds decreased cell viability in HepG2 and human colonic carcinoma cells (Caco2), but not in noncancerous human hepatocytes. This is the first report of the isolation from T. aurantiotincta of selective cytotoxic agents against cancer cells. 相似文献
90.