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61.
62.
Nishida H Nakayama M Tanaka H Kitamura M Hatoya S Sugiura K Suzuki Y Ide C Inaba T 《American journal of veterinary research》2011,72(8):1118-1123
63.
Since three decades ago it has been established by numerous investigators4,7,9) that the general Process of formation of clay minerals is different between the marine and terrestrial conditions, and that the clays of the marine origin are, in general, of illitic type, whereas those of the terrestrial are of kaolin group. In previous communications,1,2) it was reported that clay fractions of the soil and mud from the Ariake Sea are characterized by the abundance of minerals of illite-montmorin series (montmorin refers to montmorillonite group after Jackson and Sherman8), being different from most terrestrial soils in Kyushu. The matter seems to be of some importance because the Presence of montmorin, even in a relatively small quantity, is likely to exercise a considerable, influence on the behaviour and properties of the clay and the fertility of the soil. The soils of the reclaimed land of the Sea are not only productive, Whether they are clayey or sandy, but also they have been approved to be powerful improving materials for the degraded paddy soils, so-called “Akiochi” soils by several field experiments. 相似文献
64.
Jun Ohnishi Toshio Kitamura Fumihiro Terami Ken-ichiro Honda 《Journal of General Plant Pathology》2009,75(2):131-139
We studied the presence of a potential transmission barrier that blocks Tomato yellow leaf curl virus in the nonvector greenhouse whitefly, Trialeurodes vaporariorum. Because T. vaporariorum can ingest and retain the virus after acquisition feeding on an infected plant, comparable to the vector whitefly Bemisia tabaci, circumstance evidence suggested that a transmission barrier presents at location(s) where the virus moves from the digestive
tract lumen to the hemolymph. To provide direct evidence for the site of a transmission barrier in the nonvector insect, we
compared the accumulation levels and localization of the virus between the two species of whiteflies. Quantitative real-time
and conventional PCR analysis showed that accumulation of the virus during acquisition feeding and retention after a short
acquisition period were indistinguishable between the two species, but the circulation of the virus within the whiteflies
differed significantly between the species. In an immunofluorescence analysis using an antibody specific to the coat protein
of the virus, the virus was restricted to the luminal surface of the midgut epithelial cells and did not enter their cytoplasm
or that of the salivary glands in T. vaporariorum. In contrast, the virus was localized within the cytoplasm of the midgut epithelial cells and in the paired salivary glands
of B. tabaci adults. This direct evidence shows that a selective transmission barrier at the luminal membrane surface of midgut epithelial
cells in the nonvector whitefly blocks entrance of the virus into the midgut epithelial cells, resulting in incompetence as
a vector of the virus. 相似文献
65.
Hideshi Kawarazaki Masao Goto Kotaro Kato Toshio Kijima Hiroshi Kawada Keisuke Yamamoto Yuichi Takikawa 《Journal of General Plant Pathology》2009,75(3):235-240
In 2004, bacterial galls were found on the roots of carrots in Shizuoka Prefecture, Japan. Galls were about 0.1–2 cm in diameter,
light brown in color and had rough surfaces. In 2005, similar galls were found on the roots of three weeds: henbit (Lamium amplexicaule L.), Persian speedwell (Veronica persica Poir.) and leaf mustard (Brassica juncea L.). A bacterium that forms white, rough colonies was isolated from the carrot and weeds galls. The bacterial isolates had
properties identical with Rhizobacter dauci Goto and Kuwata. Phylogenetic analysis based on 16S rDNA sequences showed that the carrot isolate had the highest homology
(similarity of 100%) with that of the type strain of R. dauci. Rep-PCR genomic fingerprinting using BOX A1R primer showed that the carrot and weeds isolates were nearly identical. Pathogenicity
of the isolates was confirmed by inoculating the roots of carrots and the weeds. After 2–5 weeks, they formed galls on the
roots of the original host species and on other plant species tested. The galls were indistinguishable from those formed naturally,
and the inoculated bacterium was reisolated. Thus, the causal bacterium of carrot and weeds gall was identified as R. dauci, and the bacterium was found to have a wider host range than previously known. These weed hosts may serve as inoculum sources
for carrot bacterial gall disease. 相似文献
66.
Makoto Kiso Masahide Nohta Keiji Tanaka Norio Kurihara Toshio Fujita Minoru Nakajima 《Pesticide biochemistry and physiology》1977,7(4):341-348
The insecticidal activity of lindane analogs, in which some chlorine atoms were replaced by other groups susceptible to microsomal oxidative metabolism, was determined against mosquitos, house flies, and German cockroaches. When tested with a synergist, piperonyl butoxide, one of the methylthio analogs was as active as lindane, whereas several others were also highly active. By examining the ratio of synergized and unsynergized LD50 values (synergistic ratio value), the highly insecticidal methylthio, methoxy, and methyl analogs appear to undergo metabolic detoxication effectively in house flies. By means of in vitro metabolism experiments using microsomal fraction from house fly abdomen, the methoxy, ethoxy, and methylthio analogs were shown to be metabolized rapidly at similar rates. The synergized insecticidal activities of these compounds against various insect species relate linearly with each other, suggesting that the oxidative degradation is inhibited by the synergist to a similar extent and that the transport process to the site of action is not a limiting factor in determining the relative insecticidal activity. 相似文献
67.
Matazaemon Uchida Yasuo Irie Norio Kurihara Toshio Fujita Minoru Nakajima 《Pesticide biochemistry and physiology》1975,5(3):258-264
Lindane analogs, in which one or two chlorine atoms are replaced by various substituents, exhibit convulsive and lethal effects on Periplaneta americana (L.). Some analogs are as active as lindane. These effects are well related to the neuroexcitatory action which produces after-discharges in the central nervous system. Carbamate insecticides have potent convulsive and lethal effects on P. americana, which are also related to after-discharges produced in nerve cords. Convulsion caused by lindane analogs and carbamates is solely attributable to their neuroexcitatory effects on the nervous system, in spite of the difference in their ultimate modes of action. 相似文献
68.
Improved porcine model for Shiga toxin‐producing Escherichia coli infection by deprivation of colostrum feeding in newborn piglets 下载免费PDF全文
Toshio Sato Takashi Hamabata Eiji Takita Takeshi Matsui Kazutoshi Sawada Taishi Imaoka Nobuo Nakanishi Keizo Nakayama Takamitsu Tsukahara 《Animal Science Journal》2017,88(5):826-831
Porcine edema disease (ED) is a toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)‐producing Escherichia coli (STEC). ED occurs most frequently during the weaning period and is manifested as emaciation associated with high mortality. In our experimental infection with a specific STEC strain, we failed to cause the suppression of weight gain in piglets, which is a typical symptom of ED, in two consecutive experiments. Therefore, we examined the effects of deprivation of colostrum on the sensitivity of newborn piglets to STEC infection. Neonatal pigs were categorized into two groups: one fed artificial milk instead of colostrum in the first 24 h after birth and then returned to the care of their mother, the other breastfed by a surrogate mother until weaning. The oral challenge with 1011 colony‐forming units of virulent STEC strain on days 25, 26 and 27 caused suppression of weight gain and other ED symptoms in both groups, suggesting that colostrum deprivation from piglets was effective in enhancing susceptibility to STEC. Two successive STEC infection experiments using colostrum‐deprived piglets reproduced this result, leading us to conclude that this improved ED piglet model is more sensitive to STEC infection than the previously established models. 相似文献
69.
Toshio Sugimoto Keiichi Nomura Ryoichi Masuda Kuni Sueyoshi Yoshikiyo Oji 《Journal of plant nutrition》2013,36(10):2065-2075
Application of nitrogen (N) fertilizer at the flowering stage changed the contents of storage compounds in seeds of Soybean (Glycine max L. cvs Enrei and Tamahomare). The effects of the N application on the maturation of soybean seeds were examined by comparing changes in the contents of amino acids, sugars, water, protein, and oil in seeds from N‐dressed plants (NDS) with those from undressed plants (UDS) during maturation. The application resulted in a decrease of contents of total and some amino acids (glutamine and asparagine) in developing seeds except for at the early maturation stage and in a decreased protein content of mature seeds. On the other hand, the N application led to faster accumulation of oil in developing seeds and to an increased oil content of mature seeds. Based on these results, it was concluded that the N application at the flowering stage changed the composition of solutes imported by developing seeds and resulted in variations in the contents of storage compounds. The results indicate that it is possible to improve seed quality by fine control of N application. 相似文献
70.