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排序方式: 共有184条查询结果,搜索用时 15 毫秒
81.
82.
Growth, nutrient utilisation and gastrointestinal evacuation time using different fish meal particle sizes and dietary protein concentration was examined in Atlantic salmon. Salmon were given micro, standard or coarse grounded fish meals at two dietary protein concentrations (30 or 45%) in isolipid diets. Gastric evacuation time was influenced by fish meal particle size. Coarse ground fish meal gave a slower gastric evacuation time compared to finer ground fish meal. Specific growth rate was not influenced by the protein concentration, but there was an adverse effect of coarse ground particles. This effect may be explained by the reduced feed intake of fish fed diets containing coarse ground fish meal. Feed conversion ratio (FCR) or energy conversion ratio (ECR) was reduced with increasing dietary protein concentration. This effect was mainly caused by different energy levels in the diets and not protein/carbohydrate levels. Standard coarse fish meal gave a reduction in ECR, but there was no effect of fish meal particle size on FCR. A protein sparing effect of starch was demonstrated. Productive protein value (PPV) and productive energy value (PEV) was not affected by dietary protein concentration or fish meal particle size. Dietary protein concentration had no effect on nitrogen or fat digestibility but there was a reduction in fat digestibility when coarse ground fishmeal was used in the diets. There were only minor effects on the dressing out percentage, condition factor and fat in cutlet. 相似文献
83.
Wenwen Wang Zhigang Zhou Suxu He Yuchun Liu Yanan Cao Pengjun Shi Bin Yao Einar Ringø 《Aquaculture Research》2010,41(9):e72-e83
PCR‐denaturing gradient gel electrophoresis (DGGE) was applied to analyse the microbial community attached to the gills and skin of poly‐cultured gibel carp (Carassius auratus gibelio) and bluntnose black bream (Megalobrama amblycephala Yih) and compare these results with those detected in the rearing water. The microbiota discussed included bacteria, fungi and a specific bacterial taxa of actinomycetes was also analysed. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria, Ascomycota, Basidiomycota and some unclassified microbiota were identified. Based on our results, we concluded that: (1) the adherent bacterial/fungal communities on the gills and skin were different from those in the rearing water, (2) the bacterial/fungal diversities on fish gills were lower than that on fish skin, (3) the adherent bacterial/fungal communities on gill and skin of gibel carp were different from that of bluntnose black bream and (4) the adherent actinomycetal community showed certain similarity between the skin of different hosts. Based on our conclusions, we suggested that the topic investigated in the present study merits further investigations. 相似文献
84.
Yuchun Liu Zhigang Zhou Bin Yao Penguin Shi Suxu He Linn Benjaminsen Hølvold & Einar Ringø 《Aquaculture Research》2008,39(6):635-646
The allochthonous microbiota in the proximal and distal intestine was investigated in three groups of Atlantic salmon (Salmo salar L.) fed a commercial diet and intraperitoneally injected with (a) phosphate‐buffered saline (control), (b) lipopolysaccharide (LPS) from the fish pathogenic bacteria, Aeromonas salmonicida ssp. salmonicida, and (c) laminaran [β‐(1,3)‐d ‐glucan]. Denaturing gradient gel electrophoresis (DGGE) of the hyper variable V3 region was used to present the microbiota in different experimental groups. Sequencing and phylogenetic analysis of excised DGGE bands suggested that an intraperitoneal injection of LPS from A. salmonicida affects the allochthonous gut bacteria of Atlantic salmon to some extent, as Aeromonas enteropelogenes, Aeromonas veroni, Psychrobacter, Lactobacillus letvazi, Lactobacillus satsumensis, Pantoea, swine manure bacterium and several uncultured bacteria were unique for this group. On the other hand, the bacterial diversity of the allochthonous microbiota did not seem to be affected by injection of β‐(1,3)‐d ‐glucan. Sequences of this experimental group were most closely related to A. enteropelogenes, uncultured Escherichia and Lactobacillus aviarius ssp. aviarius. 相似文献
85.
86.
The role of monooxygenases in detoxification of the pyrethroids cypermethrin and deltamethrin was examined. Four strains of sea lice (Lepeophtheirus salmonis Krøyer) with normal or moderately reduced sensitivity towards the pyrethroids were tested in bioassays by exposure to the pyrethroid alone and in combination with an oxygenase inhibitor, piperonyl butoxide (PBO). The normal (baseline) sensitivity was considered as the sensitivity range for the two most sensitive strains. Pre‐treatment with PBO elevated the sensitivity (P < 0.01) compared with groups exposed to the pyrethroid only. A positive, but not statistically significant, correlation between the activity of haem peroxidases and the pyrethroid concentration immobilizing 50% of the parasites was demonstrated (ρ = 0.500 for deltamethrin and ρ = 0.310 for cypermethrin). The results indicate that cytochrome P450 monooxygenases are involved in detoxification of pyrethroids in sea lice. 14C‐Deltamethrin was absorbed in a lesser amount in a group of sea lice exposed to a mixture of the compound and PBO than in a group exposed to 14C‐deltamethrin alone. A significant difference could be demonstrated both immediately after exposure (P < 0.01) and 24 h after exposure (P < 0.05). No significant differences were found between groups pre‐treated with PBO and groups exposed to 14C‐deltamethrin only. 14C‐Deltamethrin was taken up mainly through the cuticle, especially the cuticle on the extremities of the ventral surface, and subsequently distributed throughout the body of the parasite. Copyright © 2005 Society of Chemical Industry 相似文献
87.
Gyrodactylus salaris Malmberg, 1957 is a major pathogen of wild Salmo salar L. parr populations in Norway, and its delimitation from non-pathogenic species is important. The present study was undertaken to test the power of chaetotaxy to differentiate between three populations belonging to both the same and different clades (as stated by mtDNA) of G. salaris, in addition to three different species of gyrodactylids (G. salaris, G. thymalli and G. caledoniensis). The gyrodactylids were processed for chaetotaxy in situ and a maximum of 50 specimens per collection site were used to construct a generalised map over the sensilla. The sensilla were found in all populations to be symmetrically distributed around the median longitudinal axis, according to a formula of 7 dorsal (34 sensilla) and 8 ventral (44 sensilla) clusters on each side of the median line. The three Norwegian populations of G. salaris were found identical, as were the population of G. thymalli. The specimens of G. caledoniensis from Scotland, however, were found to differ from the Norwegian species G. salaris and G. thymalli by the position of one sensillum in two of the clusters. A comparison of the sensillum pattern of laboratory maintained G. salaris (River Lierelva) with results obtained ten years earlier, questions the temporal stability of the chaetotaxy pattern. The present results indicate that chaetotaxy can be used to discriminate between certain Gyrodactylus spp. but not generally. 相似文献
88.
The quantitative influence of enchytraeids (Oligochaeta) and microarthropods on decomposition of coniferous raw humus in microcosms 总被引:1,自引:0,他引:1
Einar Edsberg 《Pedobiologia》2000,44(2)
Material from the organic layer of a podsol soil in a spruce stand in southern Norway was incubated for 21/2 years at 15°C in microcosms with a volume of 0.1 dm3. The soil was sterilised and reinoculated with a mixed microflora before the incubation start, and given three different faunal communities: (1) A mixed assemblage of microarthropods plus the enchytraeid Cognettia sphagnetorum (“full fauna”, FF), (2) only C. sphagnetorum (“enchytraeids”, E), and (3) no animals added (“no fauna”, NF). The respiration rate was measured during the last 14 months of incubation, and was highest in FF throughout this period. When all respiration analyses were pooled, the value for FF was 33% higher than for NF and 25% higher than for E. The substrate dry mass loss, measured twice, was also highest in FF (17% higher in FF than in both the other treatments after 11/2 years of incubation, and 31% higher than in both the other treatments after 21/2 years). Both for respiration and for mass loss, the difference between FF and the other two treatments was statistically significant, while there was no apparent difference between the E and NF treatments. There was no sign of a general rise or fall in the respiration rate during the 14 months from the first to the last analysis. The ammonium (and total N) concentration in the soil water was higher in FF and E than in NF, whereas the nitrate concentration was lowest in FF and highest in E. The higher mineralisation activity in the FF treatment was probably caused by the higher diversity of mesofauna, and perhaps also by higher diversities of microflora and microfauna accidentally introduced together with the arthropods. 相似文献
89.
Tor Arvid Breland 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):178-185
Abstract In three field trials in southern Norway, Italian ryegrass (Lolium multiflorum Lam.), white clover (Trifolium repens L.) or subterranean clover (T. subterraneuni L.) was undersown in spring grain at three N fertilizer rates and ploughed under in late October as a green manure for a succeeding spring grain crop. The content of topsoil (0-20 cm) mineral nitrogen was determined during the growth of the grain crop, after grain harvest and after ploughing. In addition, mineralization of nitrogen and carbon was measured in green-manured soil incubated at 15°C and controlled moisture conditions. During grain crop growth, ryegrass tended to reduce soil mineral N compared with the other treatments. After grain harvest, in a small-plot experiment where extra nitrate was added, ryegrass reduced soil nitrate N (0-18 cm) from 4.2 to 0.4 g m?2 within 13 days, while the clovers had negligible effect compared with bare soil. Up to 9.4 g N m?2 was present in above-plus below-ground ryegrass biomass at ploughing. In incubated ryegrass soil, there was a temporary net N immobilization of up to 0.9 g N m?2 as compared with unamended soil. In clover-amended soil, mineral N exceeded that in unamended soil by up to 5 g N m?2. 相似文献
90.
Bioavailability of triazine herbicides in a sandy soil profile 总被引:1,自引:0,他引:1
Carsten S. Jacobsen Nir Shapir Lene O. Jensen Einar H. Jensen Réne K. Juhler Jens C. Streibig Ralphi T. Mandelbaum Arne Helweg 《Biology and Fertility of Soils》2001,33(6):501-506
The bioavailability of atrazine was evaluated in a Danish soil profile (Drengsted) using a combination of soil sorption, transport and mineralisation methods as well as inoculation using Pseudomonas ADP. Sorption of atrazine decreased markedly with depth as indicated by Kd values of 5.2 l kg-1 for the upper soil and 0.1 l kg-1 for the subsoils. The transport of atrazine was evaluated using soil TLC plates and the resulting Rf values were 0.1 for the upper soil and 0.9 for the subsoil. Only a relatively small amount of atrazine leached through undisturbed soil columns taken from the upper 60 cm. Inoculating with Pseudomonas strain ADP (1᎒6 CFU g-1 dry weight soil) revealed that the degradation of 0.01 ppm atrazine was fully completed (80% mineralisation) within 10 days in the subsoil, while it reached less than 15% in the upper soil. Over a period of 500 days, a total mineralisation of 37% of added atrazine in the upper soil was found (2 mg kg-1 incubated at 20° C). However, in the subsurface soil where 0.02 mg kg-1 of atrazine was incubated at 10°C, the degradation was slower, only reaching about 12%. Terbuthylazine mineralisation was found to be temperature-dependent and low (less than 5%) in the upper soil and very much lower in the subsoil. Desethylterbuthylazine was the most frequently found metabolite. Finally, Pseudomonas strain ADP inoculated into soils from different depths increased the mineralisation of terbuthylazine dramatically. Modelling using a "two-compartment model" indicated that desorption of terbuthylazine is the limiting step for its mineralisation. 相似文献