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91.
Yongju Zhao Jiahua Zhang Hong Wei Xinming Sun Biao Mu Mingju Yu Lingbing Wang 《Tropical animal health and production》2010,42(6):1257-1262
Two experiments were undertaken to select the efficient method applied for goat estrous synchronization. In experiment 1,
a total of 120 does (Capra hircus) were divided into five groups with a randomized block design, and the does of treatment 1 were synchronized by intravaginal
sponges impregnated with 30 mg Levonorgestrel inserted for 10 days. Does of treatments 2, 3, and 4 were treated with further
injection of 25 IU follicle-stimulating hormone (FSH), 0.05 mg prostaglandin F2 alpha (PGF2α), and 25 IU FSH + 0.05 mg PGF2α at sponge withdrawal, respectively. The does in the control group (n = 40) without estrous synchronization treatment and natural estrous does were observed. In experiment 2, a total of 140 does
in five goat farms in breeding and non-breeding seasons were treated with the selective efficient procedure. The results presented
that all the employed treatments were capable of inducing and synchronizing estrous goats. According to estrous response and
economy, the use of intravaginal sponges impregnated with 30 mg Levonorgestrel and 0.05 mg PGF2α (treatment 3) is the first choice for estrous synchronization, and 95.0% of synchronized does demonstrated estrus, which
was significantly higher than that of treatment 1 (P < 0.05) and control group (P < 0.01). The percentages of ovulating of treatments 3 and 4 were the same (95.0%), which were significantly higher than that
of treatment 1 (P < 0.01). The ovulation rates among different groups were not significant (P > 0.05). When the selective procedure was applied to five goat farms, 85.7% (120/140) of does demonstrated estrus, and the
kidding percentage, litter size, and prolificacy rate were 53.6%, 0.95, and 177%, respectively. 相似文献
92.
George Rigos Alexandros Samartzis Morgane Henry Eleni Fountoulaki Efthimia Cotou John Sweetman Simon Davies Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104
The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead
sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet).
Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final
weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells,
haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst
activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue
distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200
ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly
affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory
burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300
ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication
that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point
of view. 相似文献
93.
94.
Edgar C Amar Viswanath Kiron Shuichi Satoh Nobuaki Okamoto and Takeshi Watanabe 《Fisheries Science》2000,66(6):1068-1075
SUMMARY: We attempt to assess the impact of different levels of dietary β-carotene on immune function in rainbow trout. Semi-purified diets containing 0, 40, 200, and 400 mg β-carotene/kg dry diet were fed for 12 weeks to fish with average weight of 45 g. In addition to the humoral and cellular immune parameters, growth and feed utilization were examined. There were no marked differences in growth and feed utilization showing that β-carotene was not particularly efficient in enhancing growth of rainbow trout. Of the immune parameters measured, total immunoglobulin was significantly highest for the 200 mg β-carotene fed group. Serum complement activity (alternate pathway) at 200 and 400 mg β-carotene supplementation was significantly higher than that of the unsupplemented group. An increasing trend in lysozyme activity was observed, however, the differences among the groups were not significant. Phagocytic activity was similar among diet groups except at the highest level of supplementation where it was the maximum. Oxygen radical production by peripheral blood leukocytes appeared to be lower at higher levels of carotenoid supplementation. Overall, dietary β-carotene clearly enhanced immune response parameters in rainbow trout such as serum complement activity and total plasma immunoglobulin but did not show a definite influence for the other factors examined in the present study. 相似文献
95.
96.
Matsuzaki S Cruzana MB Budipitojo T Hondo E Watanabe G Taya K Sasaki M Kitamura N Yamada J 《Anatomia, histologia, embryologia》2001,30(6):375-378
The differential localization of the inhibin beta subunits betaA and betaB in the testis of adult bull was studied using specific monoclonal and polyclonal primary antibodies. Inhibin betaA- and betaB-subunits were localized only in the Sertoli cells. The inhibin betaA-subunit was observed in the cytoplasm while the betaB-subunit was localized in the nucleus. No specific findings depending on spermatogenic stages were observed among the seminiferous tubules. Moreover, the inhibin alpha-subunit was not detected in the testis of the bulls. In addition, no inhibin subunits were detected in the Leydig cells and spermatogenic cells. These findings indicate the presence of betaA- and betaB-subunits in the bull, which may suggest a possibility that activin is produced and/or stored in the Sertoli cells and regulates spermatogenesis in an autocrine/paracrine manner. Moreover, the inhibin betaB-subunit may be produced in the nucleus but the functional meaning of this is not yet clear. 相似文献
97.
Watanabe H Koyama T Omata Y Uzuka Y Tanabe S Sarashina T Maeda R Saito A 《Veterinary parasitology》2001,99(4):287-295
In order to examine the antigenic similarity and specificity of the trail antigen of Eimeria stiedai and Etp 100, a microneme protein of Eimeria tenella, monoclonal antibodies to the trail antigen of E. stiedai sporozoites were selected by an indirect immunofluorescent antibody method. The monoclonal antibody of one clone, 3D10, reacted with the anterior portion of non-fixed sporozoites. By immunoblotting, the monoclonal antibody was found to react with a 100 kDa antigen of E. stiedai sporozoites, and a 117 kDa antigen of E. tenella sporozoites and merozoites. It was also found to react with a recombinant protein with thrombospondin-/properdin-like motifs homologous to E. tenella microneme protein Etp 100. The monoclonal antibody significantly inhibited the penetration of E. stiedai sporozoites into cultured rabbit hepatobiliary epithelial cells. These results suggest that E. stiedai sporozoites have a trail antigen, located in the anterior region on the outer surface of the sporozoites, which has an epitope with thrombospondin-/properdin-like motifs similar to E. tenella microneme protein Etp 100. This protein may play an important functional role in the process of penetration of host cells. 相似文献
98.
Arthroderma benhamiae infection in a rabbit 总被引:3,自引:0,他引:3
Saito K Kano R Nakamura Y Watanabe S Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(8):929-931
The isolate from the rabbit with dermatophytosis which was transmitted to the owners was proved to be Arthroderma benhamiae (-) by mating experiments as well as by chitin synthase 1 (CHSI) gene analysis. 相似文献
99.
Hirashima A Eiraku T Watanabe Y Kuwano E Taniguchi E Eto M 《Pest management science》2001,57(8):713-720
Some octopamine agonists were found to suppress in vitro biosynthesis of the calling pheromone of the Indian meal moth, Plodia interpunctella. Isolated pheromone-gland preparations incorporated sodium [14C]acetate at a linear rate for 3 h when incubated with the pheromone biosynthesis activating neuropeptide (PBAN). This incorporation was dependent on the dose of PBAN (up to 0.5 microM). Thin-layer chromatography of a pheromone-gland extract revealed quantitative incorporation of radioactivity into a product exhibiting the same mobility as (Z,E)-9,12-tetradecadienyl acetate, the main component of the calling pheromone of P interpunctella. Twenty-seven octopamine agonists were initially screened using a calling behaviour bioassay of female P interpunctella. Four derivatives with activity in the nanomolar range were identified which were, in order of decreasing pheromonostatic activity: 2-(2,6-diethylphenylimino)thiazolidine > 2-(2,6-diethylphenylimino)oxazolidine > 2-(2,6-dimethylphenylimino)thiazolidine > 2-(2-ethylphenylimino)oxazolidine. These compounds also showed in vitro inhibitory activity in intracellular de novo pheromone biosynthesis. The results of the present study indicate that these derivatives could provide useful information in the characterization and differentiation of octopaminergic receptor types and subtypes. 相似文献
100.
Satoshi Watanabe Kazuo Kumakura Hideki Kato Hiroyuki Iyozumi Masayuki Togawa Kozo Nagayama 《Journal of General Plant Pathology》2005,71(5):351-356
Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum. 相似文献