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51.
Natsumi ENDO Kiyosuke NAGAI Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2013,59(3):282-287
The aim of this study was to examine whether increased frequency of luteinizing hormone
(LH) pulses influences luteal progesterone (P4) secretion by measuring
progesterone concentrations at the secreted (caudal vena cava) and circulating levels
(jugular vein) in lactating dairy cows. Cows received six intravenous administrations of
2.5 μg of GnRH (gonadorelin acetate, n=4) or 2 ml saline (n=3) at 1-h intervals on 12.4 ±
0.4 (mean ± SE) days after ovulation. Blood samples were collected from the caudal vena
cava and jugular vein every 12 min for 12 h (6 h before and after treatment). During the 6
h after treatment, frequency of LH pulses (5.3 ± 0.3 and 3.0 ± 0.0 pulses/6 h) and mean LH
concentration (0.50 ± 0.06 and 0.38 ± 0.05 ng/ml) were greater (P<0.05) in GnRH-treated
cows than in saline-treated cows. Mean P4 concentration and amplitude of
P4 pulses in the caudal vena cava during the 6 h after treatment were greater
(P<0.05) in GnRH-treated cows than in saline-treated cows, but the frequency of
P4 pulses was not different between the groups. Mean P4
concentration in the jugular vein during the 6 h after treatment was also higher
(P<0.05) in GnRH-treated cows than in saline-treated cows (7.0 ± 1.3 and 5.4 ± 0.9
ng/ml). These results indicate that the increased frequency of LH pulses stimulates
progesterone secretion from the functional corpus luteum and brings about higher
P4 concentrations in the circulating blood in lactating dairy cows. 相似文献
52.
53.
Natsumi ENDO Kiyosuke NAGAI Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2013,59(2):107-114
The present study was designed to assess progesterone profiles at the secreted (caudal
vena cava) and circulating levels (jugular vein) and luteinizing hormone (LH) secretion
pattern in lactating and non-lactating cows with reference to feeding. Four lactating and
four non-lactating cycling Holstein cows were examined. Blood samples were collected
simultaneously from the caudal vena cava (via a catheter inserted from the coccygeal vein)
and the jugular vein every 15 min for 12 h (0500–1700 h) during the functional luteal
phase. Cows were fed 50% of the daily diet 6 h after the start of blood sampling. During
the 12-h sampling period, mean progesterone concentrations in the caudal vena cava did not
differ between lactating and non-lactating cows (49.0 ± 2.9 and 53.3 ± 3.7 ng/ml; mean ±
SE), whereas mean progesterone concentrations in the jugular vein in lactating cows were
higher than those in non-lactating cows (6.4 ± 0.1 and 5.6 ± 0.1 ng/ml, P < 0.001).
Lactating cows had a higher frequency of LH pulses than non-lactating cows (7.0 ± 0.7 and
4.3 ± 0.9 pulses/12 h, P<0.05). The influence of feeding was not observed on LH
profiles but was observed on progesterone profiles in both veins. Progesterone
concentrations in the caudal vena cava increased after feeding in both groups.
Progesterone concentrations in the jugular vein decreased after feeding in lactating cows
but not in non-lactating cows. These results indicate the difference in feeding-related
changes in progesterone dynamics between lactating and non-lactating cows. 相似文献
54.
Rumi Kaida Tomomi Kaku Kei’ichi Baba Masafumi Oyadomari Takashi Watanabe Sri Hartati Enny Sudarmonowati Takahisa Hayashi 《Journal of Wood Science》2009,55(5):381-386
We examined the saccharification and fermentation of meals from Acacia mangium wood, Paraserianthes falcataria wood, and Elaeis guineensis trunk. The levels of enzymatic hydrolysis of cellulose and ethanol production were highest for P. falcataria wood and lowest for A. mangium wood. Ultrasonication pretreatment of meal further increased the rates of hydrolysis and ethanol production in meal from
P. falcataria wood. Through this pretreatment, hemicelluloses (xylan and xyloglucan) and cellulose were released in the meal from P. falcataria wood. Loosening of hemicellulose associations can be expected to make P. falcataria wood more useful for bioethanol production. 相似文献
55.
Tomomi TANAKA Satoshi OHKURA Yoshihiro WAKABAYASHI Takenobu KUROIWA Kiyosuke NAGAI Natsumi ENDO Akira TANAKA Hisanori MATSUI Masami KUSAKA Hiroaki OKAMURA 《The Journal of reproduction and development》2013,59(6):563-568
The aim of the present study was to determine if the estradiol-induced luteinizing
hormone (LH) surge is influenced by the constant exposure to TAK-683, an
investigational metastin/kisspeptin analog, that had been established to depress the
pulsatile gonadotropin-releasing hormone (GnRH) and LH secretion in goats.
Ovariectomized goats subcutaneously received TAK-683 (TAK-683 group, n=6) or vehicle
(control group, n=6) constantly via subcutaneous implantation of an osmotic pump.
Five days after the start of the treatment, estradiol was infused intravenously in
both groups to evaluate the effects on the LH surge. Blood samples were collected at
6-min intervals for 4 h prior to the initiation of either the TAK-683 treatment or
the estradiol infusion, to determine the profiles of pulsatile LH secretion. They
were also collected at 2-h intervals from –4 h to 32 h after the start of estradiol
infusion for analysis of LH surges. The frequency and mean concentrations of LH
pulses in the TAK-683 group were remarkably suppressed 5 days after the start of
TAK-683 treatment compared with those of the control group (P<0.05). On the other
hand, a clear LH surge was observed in all animals of both groups. There were no
significant differences in the LH concentrations for surge peak and the peak time of
the LH surge between the TAK-683 and control groups. These findings suggest that the
effects of continuous exposure to kisspeptin or its analog on the mechanism(s) that
regulates the pulsatile and surge mode secretion of GnRH/LH are different in
goats. 相似文献
56.
Rosalina M. LAPITAN Arnel N. DEL BARRIO Osamu KATSUBE Tomomi BAN-TOKUDA Edgar A. ORDEN Alberto Y. ROBLES Libertado C. CRUZ Yukio KANAI Tsutomu FUJIHARA 《Animal Science Journal》2008,79(2):210-217
The objective of this study was to compare the carcass and meat quality in crossbred cattle and crossbred water buffalo at the same younger age and fed with high roughage in the Philippines. Ten crossbred cattle and 10 crossbred water buffalo, with an average age of 22 months (18–24 months) were used in this experiment. The animals were fed a similar diet, which consisted of 85% Napier or Para grass and 15% concentrate mixture on a dry matter basis, for 180 days before slaughter. Slaughter weight of the crossbred water buffalo was higher (P < 0.05) than that of the cattle, although the dressing percentage was significantly (P < 0.01) lower for the former than for the latter. The estimated lean yield was higher (P < 0.05) in crossbred cattle than crossbred water buffalo. The muscle fiber diameter of loin in buffalo meat was smaller (P < 0.05) than that in cattle. In regard to carcass and meat quality, the crossbred water buffalo was comparable or slightly superior to the crossbred cattle in tenderness, sensory score of color (P < 0.01) and flavor (P < 0.05). These results clearly indicate that at a younger age and even under high roughage‐based fattening rations, crossbred water buffalo are able to produce good quality meat, which will be by no means inferior to that of crossbred cattle in the Philippines. 相似文献
57.
Mohammad M. Monir Kohzy Hiramatsu Sachiko Matsumoto Kei Nishimura Chihiro Takemoto Tomomi Shioji Takafumi Watanabe Kazumi Kita Shinichi Yonekura Sang Gun Roh 《Animal Science Journal》2014,85(5):581-587
Influences of a specific dietary nutrient on glucagon‐like peptide (GLP)‐1‐containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP‐1‐containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP‐1‐immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP‐1‐immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP‐1‐immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm2, mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P < 0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP‐1‐immunoreactive cells. The protein ingestion is one of the signals that influence GLP‐1‐containing cells in the chicken small intestine. 相似文献
58.
Tomomi Kaku Kei’ichi Baba Toru Taniguchi Manabu Kurita Ken-ichi Konagaya Katsuaki Ishii Teiji Kondo Satoshi Serada Haruka Iizuka Rumi Kaida Teruaki Taji Yoichi Sakata Takahisa Hayashi 《Journal of Wood Science》2012,58(4):281-289
The transgenic expression of Aspergillus xyloglucanase cDNA (AaXEG2) with 35S promoter in the leaves of open field-grown poplars was studied. The level of xyloglucan in the transgenic poplars was decreased to 15–16% in the non-fertile soil (forest-field soil) and to 21–22% in the fertile soil (farming-field soil) compared with that of the wild-type poplars. The leaves exhibited a smaller surface area with more rounded teeth than those of the wild-type plants, similar to the sun leaf variety that was grown in the incubation room and subsequently greenhoused. The majority of total veins with water-conducting vascular bundles were shorter in the leaves of the transgenic poplars than those of the wild type. This decrease in vein length may result from a decrease in xyloglucan during leaf development, from which large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. It seems likely that the leaves of the transgenic poplars came to relax the edges of their tooth rather than extend their veins as a result of the loosening of the xyloglucan cellulose networks in the leaves. 相似文献
59.
Kazuhiro KENGAKU Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2021,67(5):332
The aim of the present study was to clarify the ovarian and hormonal dynamics after the aspiration of follicular fluid in cows with follicular cysts. Follicular fluid was aspirated from the follicular cysts and follicles that were fated to become cystic follicles and other coexisting normal follicles, respectively, in lactating cows (n = 3). After the aspiration procedure, new follicles developed and reached a diameter of 25 mm without ovulation within 13–19 days. The plasma concentrations of inhibin decreased and follicle-stimulating hormone increased rapidly after the aspiration procedure, and subsequently increased and decreased, respectively, as a new follicle grew. No luteal structures developed after the aspiration procedure, and the animals’ plasma progesterone levels remained low. The present study indicates that the cystic follicles are never luteinized by the aspiration of follicular fluid, and consequently, new follicular cysts are observed to repeatedly develop. 相似文献
60.
Kasumi Takeuchi Keisuke Tomioka Hiroshi Kouchi Tomomi Nakagawa Hisatoshi Kaku 《Journal of General Plant Pathology》2007,73(5):336-341
A wilt disease of the model legume Lotus japonicus was observed in a greenhouse in Tokyo, Japan in May 2004. Roots of diseased plants were rotted and dark brown with lesions
spreading to lower stems and leaves, resulting in rapid plant death. The causal agent was identified as Fusarium solani based on the morphology. Sequence analysis of rDNA supported the identification. Inoculation of roots of healthy plants with
conidia reproduced characteristic disease symptoms, and F. solani was reisolated from lesions, satisfying Koch’s postulates. The isolate also caused chlorotic to necrotic lesions on leaves
of healthy plants after wound-inoculation. Infection by F. solani of leaves of L. japonicus was confirmed histologically. Mycelia were observed in the intercellular spaces of parenchymatous tissues in the lesion area
and the surrounding tissues. This is the first report of fungal disease on L. japonicus satisfying Koch’s postulates. We named it “Fusarium root rot of L. japonicus” as a new disease. The compatibility of L. japonicus and F. solani is expected to form a novel pathosystem for studying interactions between legumes and fungal pathogens.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AB258993 and
AB258994. 相似文献