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991.
ABSTRACT: Blood samples were obtained from 38 wild red deer (Cervus elaphus) at two sites in Ireland and subjected to PCR analysis of the 18S rRNA gene followed by sequencing. Two fragments of the 18S rRNA gene were generated by two different PCR protocols and subsequent sequencing suggested that at least six of the deer were infected by a babesia that, in those loci, is indistinguishable from Babesia divergens, an important tick-borne pathogen of cattle and of zoonotic significance. Additionally, a B. odocoilei-like parasite was detected in three samples and a babesia that did not match any sequences in the GenBank database was found in five samples. Neither B. capreoli nor B. venatorum (EU1) were found. There have been several reports of B. divergens occurring in deer species, including red deer, roe deer (Capreolus capreolus) and reindeer (Rangifer tarandus). However, in view of recent re-sequencing of bovine-origin samples deposited previously in GenBank, it is unlikely that any of these sequences from deer are B. divergens. The present study describes the only deer piroplasm detected so far that shows complete identity with B. divergens, in just over half of the 18S rRNA gene. The entire gene of this deer parasite should be analysed and transmission experiments undertaken before the infectivity of B. divergens for red deer can be confirmed. 相似文献
992.
Kobler H Monakhova YB Kuballa T Tschiersch C Vancutsem J Thielert G Mohring A Lachenmeier DW 《Journal of agricultural and food chemistry》2011,59(13):6877-6881
Nontargeted 400 MHz (13)C and (1)H nuclear magnetic resonance (NMR) spectroscopy was used in the context of food surveillance to reveal Pinus species whose nuts cause taste disturbance following their consumption, the so-called pine nut syndrome (PNS). Using principal component analysis, three groups of pine nuts were distinguished. PNS-causing products were found in only one of the groups, which however also included some normal products. Sensory analysis was still required to confirm PNS, but NMR allowed the sorting of 53% of 57 samples, which belong to the two groups not containing PNS species. Furthermore, soft independent modeling of class analogy was able to classify the samples between the three groups. NMR spectroscopy was judged as suitable for the screening of pine nuts for PNS. This process may be advantageous as a means of importation control that will allow the identification of samples suitable for direct clearance and those that require further sensory analysis. 相似文献
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Since 1996, the emergence of Asian-origin highly pathogenic avian influenza subtype H5N1 has spurred great concern for the global poultry industry. In the United States, there is concern over the potential of a foreign avian disease incursion into the country. Noncommercial poultry operations, such as upland game bird facilities in the United States, may serve as a potential source of avian disease introduction to other bird populations including the commercial poultry industry, backyard flocks, or wildlife. In order to evaluate how to prevent disease transmission from these facilities to other populations, we examined biosecurity practices and bird movement within the upland game bird industry in the United States. Persons that held a current permit to keep, breed, or release upland game birds were surveyed for information on biosecurity practices, flock and release environments, and bird movement parameters. Biosecurity practices vary greatly among permit holders. Many facilities allow for interaction between wild birds and pen-reared birds, and there is regular long-distance movement of live adult birds among facilities. Results suggest that upland game bird facilities should be targeted for biosecurity education and disease surveillance efforts. 相似文献
998.
Savary P Gygax L Jungbluth T Wechsler B Hauser R 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(1-2):17-21
In this pilot study, we tested whether a soft mat (foam covered with a heat-sealed thermoplastic) reduces alterations and injuries at the skin and the leg joints.The soft mat in the lying area of partly slatted pens was compared to a lying area consisting of either bare or slightly littered (100 g straw per pig and day) concrete flooring. In this study we focused on skin lesions on the legs of finishing pigs as indicators of impaired welfare. Pigs were kept in 19 groups of 8-10 individuals and were examined for skin lesions around the carpal and tarsal joints either at a weight of <35 kg, or at close to 100 kg. The likelihood of hairless patches and wounds at the tarsal joints was significantly lower in pens with the soft lying mat than in pens with a bare concrete floor. Pens with a littered concrete floor did not differ compared to pens with a bare concrete floor. The soft lying mat thus improved floor quality in the lying area in terms of preventing skin lesions compared to bare and slightly littered concrete flooring. Such soft lying mats have thus the potential to improve lying comfort and welfare of finishing pigs. 相似文献
999.
Omer RA Daugschies A Romig T 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(11-12):521-527
Cystic echinococcosis is a zoonotic disease affecting mainly various species of livestock and humans. This study aims at highlighting the course of research on cystic echinococcosis in different animals and humans in Sudan (including the recently independent state of South Sudan) since the disease was first reported in 1908. Recent data about the genetic identification of the parasite in the countries in both humans and animals and its importance for future control programs is discussed. 相似文献
1000.
Emaga Thomas Happi Bindelle Jérôme Agneesens Richard Buldgen André Wathelet Bernard Paquot Michel 《Tropical animal health and production》2011,43(1):171-177
Musa sp. peels are widely used by smallholders as complementary feeds for cattle in the tropics. A study of the influence of the
variety and the maturation stage of the fruit on fermentability and metabolisable energy (ME) content of the peels was performed
using banana (Yangambi Km5) and plantain (Big Ebanga) peels at three stages of maturation in an in vitro model of the rumen.
Peel samples were analysed for starch, free sugars and fibre composition. Samples were incubated in the presence of rumen
fluid. Kinetics of gas production were modelled, ME content was calculated using prediction equation and short-chain fatty
acids production and molar ratio were measured after 72 h of fermentation. Final gas production was higher in plantain (269–339 ml
g−1) compared to banana (237–328 ml g−1) and plantain exhibited higher ME contents (8.9–9.7 MJ/kg of dry matter, DM) compared to banana (7.7–8.8 MJ/kg of DM). Butyrate
molar ratio decreased with maturity of the peels. The main influence of the variety and the stage of maturation on all fermentation
parameters as well as ME contents of the peels was correlated to changes in the carbohydrate fraction of the peels, including
starch and fibre. 相似文献