全文获取类型
收费全文 | 805篇 |
免费 | 45篇 |
专业分类
林业 | 56篇 |
农学 | 18篇 |
基础科学 | 2篇 |
239篇 | |
综合类 | 35篇 |
农作物 | 43篇 |
水产渔业 | 108篇 |
畜牧兽医 | 300篇 |
园艺 | 16篇 |
植物保护 | 33篇 |
出版年
2024年 | 2篇 |
2023年 | 7篇 |
2022年 | 8篇 |
2021年 | 37篇 |
2020年 | 30篇 |
2019年 | 41篇 |
2018年 | 27篇 |
2017年 | 23篇 |
2016年 | 42篇 |
2015年 | 27篇 |
2014年 | 26篇 |
2013年 | 66篇 |
2012年 | 77篇 |
2011年 | 61篇 |
2010年 | 34篇 |
2009年 | 27篇 |
2008年 | 45篇 |
2007年 | 50篇 |
2006年 | 47篇 |
2005年 | 41篇 |
2004年 | 31篇 |
2003年 | 35篇 |
2002年 | 33篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1989年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1972年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有850条查询结果,搜索用时 265 毫秒
101.
García-Llatas G Cercaci L Rodriguez-Estrada MT Lagarda MJ Farré R Lercker G 《Journal of agricultural and food chemistry》2008,56(2):469-475
The effect of storage on sterol oxidation of ready-to-eat infant foods was evaluated. Two different liquid infant foods (honey or fruits flavors), prepared with milk and cereals, were stored for 0, 2, 4, 7 and 9 months at 25 degrees C. Sterol oxidation products (SOP) were isolated by cold saponification, purified by silica solid-phase extraction, and analyzed by gas chromatography (GC) and GC-mass spectrometry. beta-Sitosterol was the most representative sterol, followed by cholesterol and campesterol. No significant differences in the total and single SOP content (0.8-1 mg/kg of product) were observed with respect to storage time and type of sample; the main SOP found was 7-ketositosterol (<0.2 mg/kg of product). The extent of stigmasterol oxidation (2.9%) was higher than that of cholesterol (1.9%) and beta-sitosterol (1.4%). The type and quality of raw materials, as well as the processing conditions, seem to greatly influence SOP formation and accumulation in infant foods. 相似文献
102.
Danilo G. R. Castanho Cláudia P. de Deus Jansen Zuanon Sergio Santorelli Rafael P. Leitão Fabrício B. Teresa 《Ecology of Freshwater Fish》2020,29(4):533-541
The consequences of over-exploitation may seriously impair the integrity and functioning of ecosystems. When loss of species is accompanied by the loss of ecological processes, the effects are no longer just taxonomic and may affect the stability of the environments. Ornamental fishing is one of the main economic activities of the middle Negro River. Such activity is directed to a small number of species and has the Cardinal Tetra (Paracheirodon axelrodi) as the main target species. Given the potential effects this pressure and the lack of information on its consequences, the present study analysed, through simulations, the possible effects of depletion of ornamental fish populations on the functional structure of fish assemblages, represented by functional diversity (FD) and functional redundancy (FR) indexes. We sampled 13 streams exploited by the ornamental fishery, where we collected 4,286 specimens of 110 species, of which 22 were targeted as ornamental fishing. We found that the ornamental species corresponded, on average, to 27.8% of the species and 33.3% of the FD of the assemblages. In a scenario of complete exclusion of ornamental species, local communities would lose 24.6% of the FD and reduce up to 12% of the FR. With these results, it is possible to infer that the local extinction of exploited species would cause negative impacts on the multifunctionality and the resilience of the streams ecosystems. In this way, we emphasise the need for proper management of the ornamental fisheries aiming to the sustainability of the activity and to the conservation of ecosystem functionality. 相似文献
103.
Moura-Costa LF Bahia RC Carminati R Vale VL Paule BJ Portela RW Freire SM Nascimento I Schaer R Barreto LM Meyer R 《Veterinary immunology and immunopathology》2008,126(1-2):131-141
Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a disease that affects goats and sheep, and can cause severe economic losses. In this study, four different antigenic extracts were obtained from the attenuated strain T1, which was isolated in the state of Bahia (Brazil). Forty-four Canindé breed goats were divided in five groups, each receiving a different antigen solution and saline buffer as a control. The humoral response was monitored through the identification of specific IgG by indirect ELISA and Western Blotting, and the production of IFN-gamma was followed in order to observe the activation of cellular response. After twelve weeks of antigen inoculation, the animals were challenged with 2 x 10(5)CFU of a wild strain, also isolated in Bahia, and necropsy was performed on all animals twelve weeks afterwards. It was observed that the attenuated bacteria gave a protection of 33.3%, in addition to the weak humoral response elicited. Animals inoculated with secreted antigen associated with Freund's incomplete adjuvant and oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) showed a strong humoral response, but this inoculation could not prevent the spread of challenge bacteria in the majority of animals. These results demonstrate the immunogenic potential of the attenuated T1 strain in the development of a vaccine against caseous lymphadenitis in goats. 相似文献
104.
105.
106.
107.
108.
Roser Morat Sondes Hammami Maria Teresa Paramio Dolors Izquierdo 《Reproduction in domestic animals》2019,54(5):804-807
This study examines the presence of activin IIA and IIB receptors (ActR‐IIA and ActR‐IIB) by Western blotting and immunocytochemistry in immature and IVM‐oocytes, 2 to 8‐cells embryos and blastocysts from prepubertal goats. Western blotting revealed that activin receptors are synthesized during oocyte maturation and embryo development. In the immunocytochemistry experiments, no immunostaining for either receptor was detected in oocytes while both receptors were immunolabelled in all the cells of cleaved embryos. In blastocysts, while ActR‐IIA expression appeared evenly distributed in the two cell lineages, inner cell mass and trophectoderm, the ActR‐IIB immunosignal was restricted mainly to the inner cell mass. Our findings reveal the presence of activin type II receptors (ActR‐IIA and ActR‐IIB) in in vitro matured prepubertal goat oocytes and blastocyst‐stage embryos. The expression of these receptors could be a key factor in understanding differences between competent and incompetent oocytes. 相似文献
109.
110.
Distribution of alveolar‐interstitial syndrome in dogs and cats with respiratory distress as assessed by lung ultrasound versus thoracic radiographs 下载免费PDF全文