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81.
Masashi Yamamoto Asad Asadi Abkenar Ryoji Matsumoto Tatsuya Kubo Shigeto Tominaga 《Genetic Resources and Crop Evolution》2008,55(8):1167-1173
Fluorochrome staining with chromomycin A3 (CMA) was used to characterize and compare the CMA banding patterns of chromosomes of 17 species from 13 genera of Aurantioideae,
which is one of the seven subfamilies of Rutaceae. All species used in this study had 2n = 18 chromosomes. These chromosomes
were classified into five types based on the number and position of CMA-positive bands; B: one telomeric and one proximal
band, C: two telomeric bands, D: one telomeric band, E: without bands and Dst: type D with a satellite chromosome. Each species
possessed two or four types of chromosomes and unique CMA banding patterns. The CMA banding patterns were 6D + 12E in Clausena anisata (Willd.) Hook. f.,1C + 3D + 14E in Murraya koenigii (L.) Spreng., 1C + 11D + 6E in Murraya paniculata (L.) Jack, 1B + 1C + 10D + 6E in Merrillia caloxylon (Ridl.) Swing., 1C + 9D + 7E + 1Dst in Paramignya lobata Burkill, 2C + 5D + 11E in Severinia buxifolia (Poir.) Tenore, 5D + 13E in Hesperethusa crenulata (Roxb.) Roem., 16D + 2E in Citropsis gabunensis (Engl.) Swing. et M. Kell, 14D + 4E in Citropsis schweinfurthii (Engl.) Swing. et M. Kell, 8D + 10E in Atalantia monophylla DC., 1C + 3D + 14E in Atalantia ceylanica (Arn.) Oliv., 2D + 16E in Atalantia roxburghiana Hook. f., 18E in Swinglea glutinosa (Blanco) Merr., 2D + 16E in Aegle marmelos (L.) Corr., 4D + 14E in Afraegle paniculata (Schum.) Engl., 2B + 2D + 13E + 2Dst in Feronia limonia (L.) Swing. and 3B + 9D + 6E in Feroniella oblata Swing. The advanced genera in each tribe and subtribe such as Merrillia and Feroniella showed heterochromatin (CMA-positive bands) rich chromosome configuration in general. Swinglea glutinosa without heterochromatin rich chromosome, which is considered to be the most primitive chromosome configuration, seems to
be the key genus for clarifying the evolution of the tribe Citreae. 相似文献
82.
The identification of homogeneous management zones (MZs) within a field is a basis for site-specific management (SSM). We
assessed the method of defining MZs based on the spatio-temporal homogeneity of six soil properties and above-ground biomass
data from paddy rice, winter wheat and soybean over 3 years on a farm with 124 contiguous small paddy fields. The soil data
were recorded at 372 soil sampling sites on a rectangular grid over the farm. A non-hierarchical cluster analysis was applied
to the soil data and the algorithm grouped the sites into three clusters with similar soil properties. These clusters represent
soil fertility and soil drainage. The three clusters were not randomly distributed across the fields, but formed contiguous
areas associated with landscape position. This was due to the spatial variation of the soil in the study area. We delineated
five MZs based on the spatial structure of the soil heterogeneity of the study area. The validity of the MZs was evaluated
using the biomass data from paddy rice, winter wheat and soybean in each MZ; this depended mainly on soil fertility when conditions
were dry. When the growing season precipitation was greater than the 10-year average, the biomass of winter wheat and soybean
depended on soil drainage. This suggested that the delineation of MZs for site-specific management in fields under a paddy-upland
crop rotation system should be based on several soil properties. The biomass data from the three crops over 3 years was not
effective for delimiting MZs. 相似文献
83.
Tadahide Kurokawa Takuma Okamoto Koichiro Gen Susumu Uji Koji Murashita Tatsuya Unuma Kazuharu Nomura Hajime Matsubara Shin‐Kwon Kim Hiromi Ohta Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735
The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity. 相似文献
84.
Tsutsui T Wada M Anzai M Hori T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):397-399
Artificial insemination with frozen cauda epididymal sperm was performed in cats. Sperm were transmigrated from the epididymides in 10 male cats. The mean sperm motility and viability were 67% and 82.5%, respectively, and 11.6 x 10(7) sperm were recovered. The mean sperm motility after thawing was 24.0%. Eleven female cats received unilateral intrauterine insemination of 5 x 10(7) sperm, and the conception rate was 27.3% (3/11). This was the first case of conception obtained with frozen epididymal sperm in cats. 相似文献
85.
Fisheries Science - We investigated the compositions of free amino acids (FAAs), organic acids (OAs), and nucleotides and their related compounds (NAs) in the gonads of edible sea urchins in Japan.... 相似文献
86.
Kawakami E Kuroda D Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):561-562
The peripheral blood plasma testosterone (T) levels and superoxide dismutase (SOD) activity were measured in 5 azoospermic (AZ-) beagles. The mean values in the AZ-dogs were significantly lower than in 7 control beagles (P<0.001). Subcutaneous injections of 1 mug/kg GnRH analogue three times weekly in the AZ-dogs induced significant increases in mean T level and SOD activity (P<0.05) and improvement in spermatogenesis. Thus, spermatogenic function in the dog appears to be maintained by T and normal SOD activity in the testis. 相似文献
87.
Ito H Takemura N Sonoyama K Kawagishi H Topping DL Conlon MA Morita T 《Journal of agricultural and food chemistry》2011,59(10):5771-5778
This study examined the role of degree of polymerization (DP) of inulin-fructans in modulating the interaction between lactic acid bacteria and IgA cecal secretion. Rats were fed a control diet or a diet containing one of the fructans with different DP. Consuming fructans increased the cecal IgA concentrations in the order DP4 > DP8 > DP16. Cecal lactobacilli counts were higher in DP4, DP8, and DP16, whereas bifidobacteria were higher in DP8, DP16, and DP23. Cecal IgA concentrations were correlated with cecal lactobacilli counts (P < 0.01). DP4, DP8, and DP16, but not DP23, significantly increased IgA-producing plasma cells in the cecal mucosa. IFN-γ and IL-10 production in the cecal CD4(+) T cells was enhanced solely in DP4. The results show that fructans with lower DP enhance cecal IgA secretion and increase the plasma cells and suggest that the increased lactobacilli may contribute to the stimulation of cecal IgA secretion. 相似文献
88.
Tsutsui T Mizutani T Matsubara Y Toyonaga M Oba H Hori T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(2):259-262
The mean post-thaw sperm motilities of feline frozen semen prepared with 1% OEP or 3 g/ml SLS as a cryoprotective agent, in addition to 7% glycerin, were 35.0 ± 2.4 and 37.0 ± 2.5%, respectively, showing no significant difference. On unilateral intrauterine insemination (UIUI) using these semen samples at a sperm number of 40 × 10(6), the conception rate was 70.0% (7/10) in the OEP group and 30% (3/10) in the SLS group, showing that the rate was higher in the OEP group, but the difference was not significant. It was suggested that sperm in frozen semen showing the above qualities were transferred to the contralateral uterine horn on UIUI. 相似文献
89.
Masanori KOBAYASHI Chie TSUZUKI Marika KOBAYASHI Hinano TSUCHIYA Yume YAMASHITA Kanako UENO Moe ONOZAWA Masato KOBAYASHI Eiichi KAWAKAMI Tatsuya HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1044
Oxidative stress owing to an imbalance between reactive oxygen species and antioxidants, such as coenzyme Q10 (CoQ10), is a major contributor to male infertility. We investigated the effects of the reduced form of CoQ10 (ubiquinol) supplementation on semen quality in dogs with poor semen quality. Three dogs received 100 mg of ubiquinol orally once daily for 12 weeks. Semen quality, serum testosterone, and seminal plasma superoxide dismutase (SOD) activity were examined at 2-week intervals from 2 weeks before ubiquinol supplementation to 4 weeks after the treatment. Ubiquinol improved sperm motility, reduced morphologically abnormal sperm, and increased seminal plasma SOD activity; however, it had no effect on testosterone level, semen volume, and sperm number. Ubiquinol supplementation could be used as a non-endocrine therapy for infertile dogs. 相似文献
90.
Takuya Morikawa Tatsuya Ashitani Nobuhiro Sekine Norihisa Kusumoto Koetsu Takahashi 《Journal of Wood Science》2012,58(6):544-549
In order to find new utilization method of woody wastes, we examined the bioactivities of extracts from branch heartwood of Chamaecyparis obtusa (hinoki) and compared to extracts from trunk heartwood. The bioactivities examined were antifungal activities against four fungi (Trametes versicolor, Fomitopsis palustris, Trichoderma virens, Rhizopus oryzae), and bioassay with brine shrimp (Artemia salina) which shows any allelopathic activities not measurable with fungi. Antifungal activities were observed in the hexane and ethyl acetate extracts of branch and trunk heartwood. The hexane and ethyl acetate extracts of branch and hexane extract of trunk showed strong lethality against brine shrimp. The yields of the active extracts of branch were much more than that of trunk. The identified compounds in the active extracts of branch were germacra-1-(10),5-dien-4??-ol, t-cadinol, t-muurolol, hinokiresinol, and hinokinin. Hinokiresinol and t-muurolol showed strong antifungal activities. Hinokiresinol showed bioactivities against T. virens, R. oryzae and brine shrimp. Germacra-1-(10),5-dien-4??-ol was lethal to brine shrimp. Germacra-1-(10),5-dien-4??-ol and hinokiresinol were minor components in trunk heartwood, but major components in branch. These qualitative and quantitative results suggest that the branch heartwood could be a valuable chemical resource because it contains large amounts of antifungal and allelopathic compounds. 相似文献