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11.
Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis 总被引:4,自引:0,他引:4
Dey S Mohan CM Kumar TM Ramadass P Nainar AM Nachimuthu K 《Veterinary microbiology》2004,103(1-2):99-106
A recombinant antigen-based single serum dilution enzyme-linked immunosorbent assay (ELISA) was developed to measure the specific antibody activity in sera of dogs with leptospirosis. The recombinant antigen developed and used in the assay was specific for the pathogenic serovars of Leptospira. A linear relationship was found to exist between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial-dilution method. Regression analysis was used to determine a standard curve from which an equation can be derived that allows demonstration of the mentioned correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay was proved to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). 相似文献
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Dey Daniel C. Gardiner Emile S. Schweitzer Callie J. Kabrick John M. Jacobs Douglass F. 《New Forests》2012,43(5-6):955-978
New Forests - Oaks (Quercus spp.) are one of the most important tree taxa in the northern hemisphere. Although they are dominant in mixed species forests and widely distributed, there are frequent... 相似文献
14.
Dey Daniel C. Knapp Benjamin O. Battaglia Mike A. Deal Robert L. Hart Justin L. O’Hara Kevin L. Schweitzer Callie J. Schuler Thomas M. 《New Forests》2019,50(1):11-40
New Forests - For millennia, natural disturbance regimes, including anthropogenic fire and hunting practices, have led to forest regeneration patterns that created a diversity of forest lands... 相似文献
15.
Jiangjiang Zhang Li Chang Jiangtao Yan Susmita Dey Defang Li Lining Zhao 《中国油料作物学报(英文)》2019,(2):110-117
In order to accelerate breeding progress of oil-used hemp, 8 quality traits related to lipid component were measured and analyzed in 55 representative hemp germplasms. Results showed that linolenic acid content had the highest variance coefficient of 51.67% as well as protein content had minimum variance coefficient of 4.07%. Correlation analysis showed that total oil content was significantly positively correlated to linolenic acid, while significantly negatively correlated with oleinic acid. Protein content was significantly positively correlated with linolenic acid but significantly negatively with stearic acid. Both total oil and protein content were closely correlated with other traits. The first 3 PCs from principal component analysis of 8 traits accounted for 83.53% contribution. All hemp germplasms were classified into 3 groups at the level of euclidian distance D=8.5 using cluster analysis. The above analysis and classification may provide a reliable theoretical basis and technological guidance for germplasms utilizing and accelerating breeding process. 相似文献
16.
Phytochemical composition and metabolic performance-enhancing activity of dietary berries traditionally used by Native North Americans 总被引:1,自引:0,他引:1
Burns Kraft TF Dey M Rogers RB Ribnicky DM Gipp DM Cefalu WT Raskin I Lila MA 《Journal of agricultural and food chemistry》2008,56(3):654-660
Four wild berry species, Amelanchier alnifolia, Viburnum trilobum, Prunus virginiana, and Shepherdia argentea, all integral to the traditional subsistence diet of Native American tribal communities, were evaluated to elucidate phytochemical composition and bioactive properties related to performance and human health. Biological activity was screened using a range of bioassays that assessed the potential for these little-known dietary berries to affect diabetic microvascular complications, hyperglycemia, pro-inflammatory gene expression, and metabolic syndrome symptoms. Nonpolar constituents from berries, including carotenoids, were potent inhibitors of aldose reductase (an enzyme involved in the etiology of diabetic microvascular complications), whereas the polar constituents, mainly phenolic acids, anthocyanins, and proanthocyanidins, were hypoglycemic agents and strong inhibitors of IL-1beta and COX-2 gene expression. Berry samples also showed the ability to modulate lipid metabolism and energy expenditure in a manner consistent with improving metabolic syndrome. The results demonstrate that these berries traditionally consumed by tribal cultures contain a rich array of phytochemicals that have the capacity to promote health and protect against chronic diseases, such as diabetes. 相似文献
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Effect of high inclusion of rendered animal by‐product ingredients on growth,digestibility and economic performances in climbing perch Anabas testudineus 下载免费PDF全文
Md Sakhawat Hossain Md Abdul Kader Tanwi Dey Nadia Mahjabin Sony Mahbuba Bulbul Shunsuke Koshio 《Aquaculture Research》2017,48(3):931-940
A 70‐day growth trail was conducted to investigate the effects of inclusion of high levels of meat and bone meal (MBM) and protein concentrate (PC) on growth, digestibility and economic performances of climbing perch, Anabas testudineus. Four isonitrogenous diets were formulated by lowering the level of dietary fishmeal protein at 0 (D1, control), 70 (D2), 85 (D3) and 100% (D4) with a mixture of MBM and PC protein (1:1). Triplicate groups of 300 fish (mean weight of 0.80 g) stocked in each 40 m2 pond and fed the respective test diets. A digestibility trial was conducted after the growth trial in indoor glass aquarium. The result showed that growth parameters were significantly decreased (P < 0.05) with fishmeal replacement levels. However, significant differences were not found in feed conversion ratio and survival of fish. No difference was also found in protein efficiency ratio among D1, D2 and D3. Similar to growth parameters, total fish production was highest in D1, intermediate in D2 and D3; and lowest in D4. Apparent digestibility coefficients of dry matter, protein and lipid were highest (P < 0.05) in D1 and lowest in D4. The economic analysis revealed that the benefit cost ratio was ranked by D3 (1.81), D2 (1.71), D1 (1.66) and D4 (1.46) respectively. Upon considering the overall performances and unavailability of finite protein sources, it can be concluded that 70–85% fishmeal could be replaced with a mixture of MBM and PC (1:1) in practical diets for climbing perch. 相似文献
19.
Panja Suraj Mondal Kongkong Kar Rup Kumar Dey Pradip Chandra Dey Narottam 《Journal of Crop Science and Biotechnology》2023,26(1):87-95
Journal of Crop Science and Biotechnology - Assamese Bora rice lines are valuable genetic resource for their socio-economic importance and traditional usage. Being lowland rice lines of upper... 相似文献
20.
Shiv Ram Singh Dilip Kumar Kundu Pradip Dey Bikash Singha Mahapatra 《Communications in Soil Science and Plant Analysis》2017,48(18):2170-2192
The scarcity of non-renewable fertilizers resources and the consequences of climate change can dramatically influence the food security of future generation. Introduction of high yielding varieties, intensive cropping sequence and increasing demand of food grains day-by-day, application of recommended dose of fertilizers could not fulfill our targets due to outdated fertilizers recommendations are yet in practice. It not only alters soil quality, nutrient balance, microbial and enzymatic ecology but also affected productivity and sustainability of rice in Gangetic alluvial soils of India. The effect of fertilizers application based on “fertilizing the soil versus fertilizing the crop” which insure real balance between the applied and available soil nutrient is urgently needed. Hence, the present study was conducted during three consecutive crop seasons (2010, 2011, and 2012) to assess the effect of imbalance and balance fertilization based on initial soil test values and targeted yields, and to determine the effect of farmyard manure (FYM) when superimposed with balanced fertilizers on identification of minimum data set for the development soil quality, nutrient acquisition, and grain yield of rice. The six fertilizer treatments were laid out in a randomized block design with three replications. The treatments were: T1-control (no fertilization), T2-farmyard manure @ 5 t ha?1, T3-farmers practice (60:30:30 kg N:P2O5:K2O ha?1), T4-precise application of mineral fertilizers based on initial soil test values (77:24:46 kg N:P2O5:K2O ha?1) for targeted grain yield of 4.0 t ha?1, T5-precise application of mineral fertilizers based on initial soil test values (74:23:43 kg N:P2O5:K2O ha?1) plus FYM (5 t ha?1) for targeted grain yield of 4.0 t ha?1 and T6-precise application of mineral fertilizers based on initial soil test values (135:34:65 kg N:P2O5:K2O ha?1) for targeted rice grain yield of 5.0 t ha?1. Result revealed that the targeted rice grain yield of 4.0 and 5.0 t ha?1 was achieved in T4 and T6 treatments with 1.59% (4.06 t ha?1) and –3.40% (4.83 t ha?1) deviations, respectively. T4, T5, and T6 significantly increased crop growth, nutrient uptake, available P (Pa) and K (Ka) and augmented rice grain yield by 10.6, 20.2 and 31.6%, respectively, over T3. Microbial biomass carbon, soil respiration and enzymatic activity were enhanced significantly in T5 as compared to T6. Highest soil quality index was found in T5 (0.95) followed by T6 (0.90) and, lowest was in T1 (0.63). The contribution of minimum data set (MDS) toward the SQI was in the descending order of ALP (30.6%) > SOC (21.5%) > Ka (11.3%) > PSM (9.68%) > Na (8.51%). Overall, rice yield and soil quality was improved by using balance fertilization based on fertilizing the crop Vs fertilizing the soil in alluvial soils of India. 相似文献