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81.
Hirokazu Osabe Yasuo Morishima Yukihisa Goto Kazuhisa Masamoto Yoshiaki Nakagawa Toshio Fujita 《Pest management science》1992,34(1):17-25
A number of substituted 4-pyridone-3-carboxanilide derivatives show various degrees of light-dependent herbicidal activity. The effects of substituents at the anilide moiety on the activity against Echinochloa oryzicola were analysed quantitatively with physicochemical substituent parameters. The activity was shown to vary parabolically with total hydrophobicity (Σπ) of the anilide ring substituents as well as with the steric parameter (Es) of the ortho-substituents. The Es parameter for only the bulkier ortho-substituent, with the more negative value, but not for the two, was significant. The effect of substituents at the 1-position was expressible by indicator variables assigned to each 1-substituted series, the physicochemical rationalization being left for future analyses. 相似文献
82.
Hirokazu Osabe Yasuo Morishima Yukihisa Goto Toshio Fujita 《Pest management science》1992,35(2):187-200
Using a set of representative members selected from 4-pyridone-3-carboxanilides and ortho-chlorinated diphenyl ethers exhibiting light-dependent herbicidal activity, we examined the three-dimensional structure-activity relationships quantitatively. The most stable conformation of each compound, regarded as the ‘active conformation’, was determined by either semi-empirical or ab initio molecular orbital calculations. With a hypothesis defining a common substructural skeleton between the two different compound series. each molecule was superimposed. We first analysed the structure-activity relationship using an index for the substructure shape similarity according to the superimposed conformations. After finding the relevance of the hypothesis, we examined the three-dimensional structure-activity relationship using the comparative molecular field analysis procedure. The results suggested that the two different series of compounds have a common region of the receptor site. The variations in the light-dependent herbicidal potency were governed by hydrophobicty and three-dimensional steric and electronic field parameters of molecules participating in the transport process and the interaction with the receptor site. The result was consistent with that derived from our previous quantitative analysis with the use of free-energy-related substituent parameters and the traditional regression procedure for a large number of pyridone-carboxanilides. 相似文献
83.
84.
Characterization of myofibrillar adenosine triphosphatase activity and liberation of actin from myofibrils upon heating chicken breast meat 下载免费PDF全文
Masanori Matsuishi Yoshitaka Eda Emi Saito Shohei Yamamoto Kenji Kanamori Yuto Goto Yutaro Kobayashi Akihiro Okitani 《Animal Science Journal》2018,89(3):597-605
Denaturation of actin and myosin in myofibrils induced by heating at 50°C was investigated to reveal the mechanism of irreversible liberation of actin from myofibrils on heating at lower temperatures than conventional cooking. Denaturation of these proteins was determined by Mg2+‐ATPase (adenosine triphosphatase) and Ca2+‐ATPase activities. When minced meat was heated for 20 min, actin was liberated accompanying denaturation of 80% of actin and 50% of myosin. Heating of the myofibrillar fraction (MFF) isolated from meat homogenate induced much slower denaturation of actin than myosin. When MFF was heated with sarcoplasmic fractions, denaturation of actin was facilitated, suggesting that sarcoplasmic fractions contain factors to facilitate actin denaturation. Inosine‐5′‐monophosphate, a component of sarcoplasmic fractions, was shown to have no effect on actin and myosin denaturation. These results suggest that heating meat at 50°C dissociates binding (‘Bond A’) between actin and myosin participating in ATPase activities, resulting in denaturation of both proteins under influence of sarcoplasmic components. Although denaturation of actin and myosin disrupted Bond A, actin was not liberated simultaneously, suggesting the presence of another bond (‘Bond B’, more heat‐stable than Bond A) between both proteins and necessity of disruption of Bond B for actin release from myofibrils. 相似文献
85.
During the growth of sago palm (Metroxylon sagu Rottb.), primary suckers appear from the main stem (MS) of transplanted suckers. Then, secondary suckers appear from the primary suckers. After the MS (trunk) is first harvested 10 and several years after transplantation, trunks can be harvested persistently using the primary, secondary, and subsequent suckers, which are designated as derivative suckers, growing with the MS. However, little knowledge exists about the growth behavior of derivative suckers. This study clarified how derivative suckers, especially the primary and secondary suckers, spread in the horizontal direction, and how they form a plant with the MS during the creeping growth stage. Most derivative suckers crept in the direction of about 70° subtended by the mother stem. However, two primary suckers that appeared early after transplantation crept in an obtuse angle to the creeping direction of the MS. As the reason for this obtuse angle direction, we considered the following four factors: (1) the existence of petiole, (2) distance from the sucker to the ground surface, (3) enlargement of the MS, and (4) space for sucker growth. The growth behaviors of the two primary suckers and the other derivative suckers differed. Therefore, in sago palm cultivation, the two primary suckers which appeared first from the MS were very important for formation of the framework of the plant with the MS, in terms of efficient utilization of space for the growth of derivative suckers. 相似文献
86.
Nagao Y Nishio Y Shiomoda H Tamaru S Shimojima M Goto M Une Y Sato A Ikebe Y Maeda K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):699-705
Canine distemper virus (CDV), a morbillivirus that causes one of the most contagious and lethal viral diseases known in canids, has an expanding host range, including wild animals. Since December 2009, several dead or dying wild raccoon dogs (Nyctereutes procyonoides) were found in and around one safari-style zoo in Japan, and CDV was isolated from four of these animals. In the subsequent months (January to February 2010), 12 tigers (Panthera tigris) in the zoo developed respiratory and gastrointestinal diseases, and CDV RNA was detected in fecal samples of the examined tigers. In March 2010, one of the tigers developed a neurological disorder and died; CDV was isolated from the lung of this animal. Sequence analysis of the complete hemagglutinin (H) gene and the signal peptide region of the fusion (F) gene showed high homology among these isolates (99.8-100%), indicating that CDV might have been transmitted from raccoon dog to tiger. In addition, these isolates belonged to genotype Asia-1 and had lower homology (<90%) to the vaccine strain (Onderstepoort). Seropositivity of lions (Panthera leo) in the zoo and wild bears (Ursus thibetanus) captured around this area supported the theory that a CDV epidemic had occurred in many mammal species in and around the zoo. These results indicate a risk of CDV transmission among many animal species, including large felids and endangered species. 相似文献
87.
Minami Goto Keishi Owaki Akihiro Hirata Tokuma Yanai Hiroki Sakai 《Veterinary and comparative oncology》2019,17(3):285-297
Tumour necrosis factor‐related apoptosis‐inducing ligand (TRAIL) is an apoptosis‐inducing cytokine that shows potential therapeutic value for human neoplasms, and is effective in some canine tumours; however, its potential for killing canine hemangiosarcoma (HSA) cells is unknown. Thus, we evaluated the proapoptotic effect of TRAIL in nine canine HSA cell lines. Cells (JuA1, JuB2, JuB2‐1, JuB4, Re11, Re12, Re21, Ud2 and Ud6) were cultured with three recombinant human TRAILs (rhTRAILs): TRAIL‐TEC derived from Escherichia coli, TRAIL‐TL derived from mammalian cells and isoleucine zipper recombinant human TRAIL (izTRAIL) containing an isoleucine‐zippered structure that facilitates trimerization. TRAIL‐TEC did not decrease the cell viability in any of the cell lines tested, whereas the other two rhTRAILs effectively decreased the viability of all cell lines as assessed by the WST‐1 assay. In canine HSA cells, izTRAIL induced apoptosis more effectively than TRAIL‐TL. In JuB4, Re12, and Ud6 cells, izTRAIL increased the activation of caspase‐3 and caspase‐8 and caused poly (ADP‐ribose) polymerase degradation. Moreover, izTRAIL treatment increased the proportion of Annexin V+/ Propidium iodide (PI)? apoptotic cells and nuclear fragmentation in izTRAIL‐sensitive cells. These results show that rhTRAIL can induce apoptosis in canine HSA cells, but the sensitivity of TRAIL was different depending on the cell lines. Therefore, TRAIL could be an effective therapeutic agent against canine HSA, but the specific mechanism of resistance should be determined to clarify under what conditions this treatment would be most effective. 相似文献
88.
Monotonic and reversed cyclic loading tests were conducted on dowel-type timber joints with varying degrees of wood decay
due to Fomitopsis palustris (Berk. et Curt.), a brown rot fungus, and the effect of decay on various shear performances of dowel-type joints was investigated.
For joints affected by the brown rot fungus, the initial stiffness, yield load, and maximum load of dowel-type joints were
significantly decreased, even with a small mass loss of wood. The reductions in shear performance were the largest for initial
stiffness, followed by yield load and maximum load, in that order. For a 1% reduction of the yield load, initial stiffness
and maximum load showed reductions of 1.15% and 0.77%, respectively. When dowel-type joints that had been exposed to the brown
rot fungus were subjected to reversed cyclic loading, the gap between the dowel and the lead hole of the wood was increased
and equivalent viscous damping was decreased. These results indicate that decay around the dowel lead hole especially affects
the load-displacement behavior at small displacement level, and dowel-type joints under cyclic loading have very low resistance
to forces acting on the wooden structure.
Part of this report was presented at the 5th Symposium on Timber Bridges of the Japan Society of Civil Engineers, Tokyo, July
2006; the 56th Annual Meeting of the Japan Wood Research Society, Akita, August 2006; and the Annual Meeting of the Architectural
Institute of Japan, Fukuoka, August 2007 相似文献
89.
Toshinori Sako Akihiro Mori Peter Lee Hiroshi Goto Hiroko Fukuta Hitomi Oda Kaori Saeki Yohei Miki Yuki Makino Katsumi Ishioka Hisashi Mizutani Yuzo Kojima Satoshi Koikeda Toshiro Arai 《Veterinary research communications》2010,34(2):161-172
Indigestible oligosaccharides have been shown to normalize blood glucose and insulin concentration thereby promoting good health and preventing diseases, such as diabetes. Transglucosidase (TG, α-glucosidase, enzyme code (EC) 3.2.1.20) is an enzyme capable of converting starch to oligosaccharides, such as iso-malto-oligosaccharides from maltose, via the action of amylase. The aim of this study was to evaluate whether oral administration of TG with maltose or dextrin is capable of reducing post-prandial serum glucose concentration in experimentally streptozotocin (STZ)-induced diabetic dogs fed on a high-fiber diet. Five healthy and five STZ-induced diabetic dogs were employed in this study. TG supplementation with dextrin or maltose had no detrimental effect in healthy dogs. In fact, TG and dextrin exhibited a flatlined serum glucose pattern, while reducing mean post-prandial serum insulin and glucose concentration as compared to control diet alone. When TG supplementation was tested in STZ-induced diabetic dogs under the context of a high fiber diet, a 13.8% and 23.9% reduction in mean glucose concentration for TG with maltose and dextrin, respectively was observed. Moreover, TG with dextrin resulted in a 13% lower mean post-prandial glucose concentration than TG with maltose, suggesting that dextrin may be a more efficient substrate than maltose when used at the same concentration (1 g/kg). Our results indicate that TG supplementation with diet can lead to lower postprandial glucose levels versus diet alone. However, the efficacy of TG supplementation may depend on the type of diet it is supplemented with. As such, TG administration may be useful for preventing the progression of diabetes mellitus and in its management in dogs. 相似文献
90.
Jin J Xu D Narongwanichgarn W Goto Y Haga T Shinjo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):273-276
The 16S-23S rRNA intergenic spacer regions (ISRs) of Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme were characterized. Products of two sizes, about 360 bp (small) and 530 bp (large), were generated by PCR amplification from the 16S-23S rRNA ISR of all the strains tested. The large and small 16S-23S rRNA ISRs of F. necrophorum exhibited a level of sequence similarity of 93.9% to 99.7% and 94.2% to 98.6% homologies within the species, respectively. Only the large spacer regions in these bacteria contained one or two tRNA genes. F. necrophorum subsp. necrophorum contains the isoleucine and alanine tRNA gene, whereas F. necrophorum subsp. funduliforme contains the isoleucine tRNA gene. 相似文献