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排序方式: 共有391条查询结果,搜索用时 343 毫秒
61.
Uechi M Harada K Mizukoshi T Mizuno T Mizuno M Ebisawa T Ohta Y 《Veterinary surgery : VS》2011,40(4):413-417
Objective: To describe surgical repair of a large atrial septal defect (ASD) in a cat. Study Design: Clinical report. Animal: A 3‐year‐old, 3.3 kg, intact male Japanese domestic short‐haired cat. Methods: A 10.2‐mm‐diameter ASD detected by echocardiography was surgically corrected because pulmonary vascular resistance‐to‐systemic vascular resistance ratio (Qp/Qs) was 3.2. Using cardiopulmonary bypass (CPB), open surgical repair was achieved with an expanded polytetrafluoroethylene (e‐PTFE) graft. The priming volume of the CPB circuit was minimized by cutting the CPB tubing, and partially replacing the priming fluid with whole cat blood. To prevent hemodilution associated with use of cardioprotective agents, surgery was performed on the beating heart. Results: At 1‐year echocardiographic evaluation, the repair was intact, and at 3 years, the cat was alive without need of medication. Conclusions: Large ASD in a cat can be repaired using e‐PTFE under CPB. 相似文献
62.
Yusaku Tsugami Norihiro Suzuki Manabu Kawahara Takahiro Suzuki Takanori Nishimura Ken Kobayashi 《Animal Science Journal》2020,91(1)
This study attempted to establish a culture model to recreate the milk production pathway in bovine mammary epithelial cells (BMECs). BMECs were isolated from Holstein cows (nonlactating, nonpregnant, and parous) and were stored by cryopreservation. To separate the apical and basolateral compartments, BMECs were cultured on a cell culture insert with a collagen gel in the presence of bovine pituitary extract and dexamethasone to induce milk production and tight junction (TJ) formation. The culture model showed the secretion of the major milk components, such as β‐casein, lactose, and triglyceride, and formed less‐permeable TJs in BMECs. Moreover, the TJs were distinctly separated from the apical and basolateral membranes. Glucose transporter‐1, which transports glucose into the cytoplasm through the basolateral membrane, localized in the lateral membrane of BMECs. Toll‐like receptor‐4, which binds to lipopolysaccharide in the alveolar lumen in mastitis, localized in the apical membrane. Beta‐casein was mainly localized near the Golgi apparatus and the apical membrane. Moreover, milk components were almost secreted into the upper chamber of the cell culture insert. These findings indicate that this model has clear cell polarity as well as in vivo and is effective to study of milk production and the blood–milk barrier in lactating BMECs. 相似文献
63.
Naoki Isobe Shoko Matsukawa Kota Kubo Kazutoshi Ueno Toshihisa Sugino Takahiro Nii Yukinori Yoshimura 《Animal Science Journal》2020,91(1)
The present study was conducted to examine whether colostrum supplementation in peripartum goats increases the antimicrobial peptides in their milk. Goats were orally administered 2 ml of colostrum whey products (colostrum group) or water (control group) daily, from 2 weeks before until 2 weeks after kidding. Body weights of mothers and kids were measured. Blood, milk, and fecal samples were collected from the mothers, and blood samples were collected from the kids. Concentrations of milk antimicrobial peptides (beta‐defensin, cathelicidin, lactoferrin, S100A7, lactoperoxidase, and immunoglobulin A [IgA]) were determined. IgA and nutritional parameters (glucose, total cholesterol, triglyceride, ketone bodies, and non‐esterified fatty acids) were also determined in the blood of mothers and kids. Milk IgA and lactoferrin concentrations were higher in the colostrum group than in the control group. Conversely, lower milk concentrations of S100A7 were observed in the colostrum group than that in the control group. Plasma IgA concentrations were higher for kids from the colostrum group than for those from the control group. These results suggest that oral administration of colostrum in pregnant goats increases IgA concentration in postpartum milk, which can subsequently improve the health of their kids. 相似文献
64.
Takehiro Hachiken Kei Sato Takahiro Hasegawa Katsuyuki Ichitani Makoto Kawase Kenji Fukunaga 《Genetic Resources and Crop Evolution》2013,60(4):1559-1570
To elucidate diversity and evolution of the Waxy gene in foxtail millet, Setaria italica, we analyzed sequence polymorphism of Waxy gene in 83 foxtail millet landraces collected from various regions covering the entire geographical distribution of this millet in Europe and Asia. We found a unique geographic distribution pattern at the sequence level of gene haplotypes and also found a large diversity in East Asian landraces. We also found a higher degree of genetic polymorphism in a non-waxy phenotype than in other low amylose types, supporting the hypothesis that low amylose types recently originated from non-waxy type. 相似文献
65.
Taiichiro Hattori Kaori Sonobe Hideki Araki Shinobu Inanaga Ping An Shigenori Morita 《Journal of plant nutrition》2013,36(8):1482-1495
In this study, it was verified whether silicon (Si) affected plant hydraulic resistance, which was one of the significant factors affecting water uptake. Sorghum bicolor (L.) Moench. was grown hydroponically under varying silicon levels and exposed to osmotic stresses. Under osmotic stress, reduction in growth, photosynthesis, and transpiration were alleviated as supplied silicon levels increased. These alleviative effects were ascribed to enhancement of water uptake. Although shoot/root ratio was not affected by silicon, estimated apparent hydraulic resistance was lower in silicon-supplied sorghum than silicon-deficient one under osmotic stress. Simultaneous measurement of transpiration and water uptake rates indicated that under osmotic stress silicon-deficient sorghums showed unbalanced water relation that transpiration rate exceeded water uptake rate, while they were balanced in silicon-supplied sorghums. The results indicated that silicon improved hydraulic resistance, allowing sorghum to avoid from decrease in water uptake rate that happens to silicon-deficient sorghum under water stress. 相似文献
66.
Haruto Sasaki Takahiro Hara Satoshi Ito Naoko Uehara Han-Yong Kim Mark Lieffering Masumi Okada Kazuhiko Kobayashi 《Field Crops Research》2007
Increasing global atmospheric CO2 concentrations are expected to influence crop production. To investigate the effect on rice (Oryza sativa L.), plants were grown under ambient CO2 (AMB) or free-air CO2-enrichment (FACE) at CO2 concentrations ranged from 275 to 365 μmol mol−1 above AMB. We supplied 13CO2 to the plants at different growth stages so we could examine the contribution of carbohydrate stored during the vegetative stage or newly fixed carbohydrate produced during the grain-filling stage to ear weight at grain maturity. In plants supplied with 13C at the panicle-initiation or pre-heading stages, plants grown under FACE had more starch in the stems at heading, but there was no difference in stem 13C content. Furthermore, there were no differences between treatments in whole-plant 13C contents at heading and grain maturity. In contrast, plants supplied with 13C at the grain-filling stage had more 13C in the whole plant and the ears at grain maturity under FACE than under AMB, indicating that the increased amount of photosynthate produced at the grain-filling stage under CO2 enrichment might be effectively stored in the grains. Furthermore, regardless of when the 13C was supplied, plants had more 13C in starch in the ears at grain maturity under FACE than under AMB. Therefore, CO2 enrichment appears to promote partitioning of photosynthate produced during both vegetative and grain-filling stages to the grains. 相似文献
67.
Shimotori Koichi Yamamoto Haruki Imai Akio Kohzu Ayato Okamoto Takahiro 《Journal of Soils and Sediments》2021,21(2):1222-1231
Journal of Soils and Sediments - Sediment oxygen demand (SOD) measurement currently requires a long preparation time and bulky experimental equipment, which represent major obstacles to conducting... 相似文献
68.
Assessment of the water quality of two rivers in Hanoi City and its suitability for irrigation water
Nguyen Thi Lan Huong Masami Ohtsubo Loretta Li Takahiro Higashi Motohei Kanayama 《Paddy and Water Environment》2008,6(3):257-262
The To Lich and Kim Nguu Rivers in Hanoi City are the main sources of irrigation water for suburban agricultural land and
fish farm. Wastewater from the industrial plants located along these rivers has been discharged, and has degraded the water
quality of the rivers. This study describes the chemical properties of water from the rivers, focusing on heavy metal pollution
and the suitability of water quality for irrigation water. Water from the rivers was heavily polluted with organic matter
and heavy metals such as Pb, Cu, Zn, Cr, Cd and Ni. Dissolved oxygen, chemical oxygen deman, and total suspended solids, and
the concentrations of all heavy metals exceeded the Vietnamese standard for surface water quality in all investigated sites.
The concentrations of some heavy metals such as Cu, Cd, Cr and Ni were above the internationally recommended WHO maximum level
for irrigation water. A wide variation in the heavy metal concentration of water due to metal types is the result of wastewater
discharged from different industrial sources. 相似文献
69.
70.
Masato MIYAKE Shinichiro HAYASHI Yoshikazu TAKETA Shunsuke IWASAKI Kouichi WATANABE Shyuich OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2010,81(2):223-229
Myostatin (MSTN) is a negative regulator during muscle differentiation, whereas insulin‐like growth factors (IGFs) are essential for muscle development. MSTN and IGFs act oppositely during myogenesis, but there is little information on the mutual relationship of MSTN and IGFs. The present study was conducted to examine whether MSTN affects IGF expression during early myogenesis in cattle. IGF‐1 mRNA was similarly expressed in M. longissimus thoracis of double‐muscled (DM) and normal (NM) Japanese shorthorn cattle. IGF‐2 mRNA expression was consistently higher in the normal and regenerating muscle of DM cattle than those of NM cattle. When myoblasts were isolated from regenerating M. longissimus thoracis, IGF‐2 mRNA expression showed a significant increase in differentiating DM derived myoblasts (DM‐myoblasts) as compared with differentiating NM derived myoblasts (NM‐myoblasts). An addition of recombinant mouse myostatin (rMSTN) to myoblast cultures attenuated IGF‐2 mRNA expression and decreased myotube formation, but did not effect IGF‐1 mRNA expression. An activin‐like kinase (ALK) inhibitor, SB431542, mediates MSTN action, suppressed the translocation of Smad2/3 into the nucleus in DM‐myoblasts, and restored the attenuated IGF‐2 mRNA expression and the decreased myotube formation induced by rMSTN in myoblast cultures. The findings indicate that MSTN may negatively regulate myoblast differentiation by suppressing IGF‐2 expression via ALK‐Smad signaling. 相似文献