The effect of initial soil water content and rainfall intensity on near-surface soil hydrologic conductivity: A laboratory investigation

This paper investigates the influence of rainfall intensity and initial soil water content on changes in the near-surface soil hydraulic conductivity. While numerous papers have examined surface sealing, this paper outlines two important innovations: the design and application of time-domain reflectometry probes with the ability to measure and record soil water content at very short time and length scales; the design and application of stainless steel tensiometers to measure soil water potential at the same, very short, time and length scales. These permit the Richards Equation to be applied and the near-surface hydraulic conductivity computed. An exponential curve was fitted to the temporal changes in hydraulic conductivity, as both a function of water potential or soil water content, with a high degree of fit. As rainfall intensity increased, the degree of scatter about the best-fit line increased, probably due to the ability of high intensity rainfall to disrupt the near-surface soil structure. While utilising deterministic models, such as the Richards Equation, may be appropriate at the column scale there are practical difficulties due to the very small length scale variability in each of the input variables. The experimental results detailed here indicate that even with data at very short time and length scales it is still not possible to utilize the Richards Equation, or at least, it may be difficult to transfer the laboratory results to the field due to the effect of the specific laboratory experimental conditions on the results.     

Learning and teaching animal handling at the University of Sydney's Faculty of Veterinary Science

Handling animals in veterinary contexts can present unique challenges that are difficult to anticipate. Skillful handling of animals in health and disease enhances client and patient relations, while inappropriate handling affects diagnostic parameters and is detrimental to a compromised patient's ability to cope. This article describes animal-handling opportunities in the veterinary curriculum at the University of Sydney, Australia, with an overview of the core handling skills required at graduation.     

Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins

The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm‐raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive‐sequence‐mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep‐PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep‐PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.