Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor

Bacteria have evolved a set of highly specialized proteins to capture iron in iron-depleted environments. The acquisition and uptake of iron present in the extracellular milieu of eukaryotic organisms is indispensable for the growth and survival of microbial pathogens in the course of infection. Haemophilus parasuis is the causative agent of Gl?sser disease, which is responsible for considerable financial losses in pig-rearing worldwide. To gain insight into the mechanisms involved in siderophore-mediated iron uptake in H. parasuis, genes in the H. parasuis ferric hydroxamate uptake (Fhu) region were amplified in the work being reported here. As has been described in A. pleuropneumoniae, an Fhu genomic region was also present in H. parasuis, being composed of four potential consecutive open reading frames (ORF) designated as fhuC, fhuD, fhuB, and fhuA, respectively. By immunoblotting, using a cross-reactive polyclonal antibody raised against Actinobacillus pleuropneumoniae FhuA protein, it was demonstrated that this protein was constitutively expressed in H. parasuis and its level of expression was not modified under conditions of restricted iron availability. This is the first report describing the presence of the fhu genes in H. parasuis. Our results indicate that FhuA protein expression is not affected under iron-restricted conditions, however, it is one of the targets of the humoral immune response.     

COLOR DOPPLER AND DOPPLER SPECTRAL ANALYSIS IN THE SPINAL CORD OF NORMAL DOGS

Doppler ultrasonography of the spinal cord was performed in 34 normal, anesthetized dogs following hemilaminectomy. This study was part of an investigation to evaluate the efficacy of a 21-aminosteroid compound and high dose methylprednisolone for the treatment of spinal cord trauma. Grey-scale images of the canine spinal cord were similar to those described for the spinal cord of people. Doppler waveforms of intraparenchymal spinal arteries exhibited high end diastolic blood flow velocities, indicating low resistance to flow. Doppler values (mean ± SD) for arteries immediately ventrolateral to the central canal were: Peak Systolic Velocity = 5.78 ± 2.5 cm/sec, Minimum Diastolic Velocity = 3.5 ± 1.62 cm/sec, Mean Velocity = 4.45 ± 1.96 cm/sec, Minimum Diastolic Velocity = 3.5 ± 1.62 cm/sec, Mean Velocity = 4.45 ± 1.96 cm/sec, Systolic/Diastolic ratio = 169 ± 0.19, Pulsatility Index = 0.53 ± 0.09, and Resistance Index = 0.4 ± 0.06.     

Detection of all Chlamydophila and Chlamydia spp. of veterinary interest using species-specific real-time PCR assays

The aim of the present study was to analyse the occurrence of chlamydiae in several mammalian host species. Clinical samples that previously tested positive in a Chlamydiaceae-specific real-time PCR were retested using six species-specific real-time PCR assays to identify the chlamydial species involved. Chlamydophila (Cp.) abortus was the agent most frequently found in cattle, sheep, horses, goats, and pigs. Detection in cattle of Cp. psittaci (11% of samples) and Chlamydia (C.) suis (9%), as well as Cp. psittaci in a goat sample was somewhat unexpected. DNA of two different chlamydiae was identified in 56 (12.7%) of 440 samples tested. Cp. felis was the predominant species found in cats, while in guinea pigs and rabbits only Cp. caviae was detected. Interestingly, the latter two pathogens were also identified in samples from dogs. The data show that mixed chlamydial infections are not rare and suggest an extended host range of individual species.     

Potential new genes for resistance to <Emphasis Type="Italic">Mycosphaerella graminicola</Emphasis> identified in <Emphasis Type="Italic">Triticum aestivum</Emphasis> × <Emphasis Type="Italic">Lophopyrum elongatum</Emphasis> disomic substitution lines

Lophopyrum species carry many desirable agronomic traits, including disease resistance, which can be transferred to wheat by interspecific hybridization. To identify potentially new genes for disease and insect resistance carried by individual Lophopyrum chromosomes, 19 of 21 possible wheat cultivar Chinese Spring × Lophopyrum elongatum disomic substitution lines were tested for resistance to barley yellow dwarf virus (BYDV), cereal yellow dwarf virus (CYDV), the Hessian fly Mayetiola destructor, and the fungal pathogens Blumeria graminis and Mycosphaerella graminicola (asexual stage: Septoria tritici). Low resistance to BYDV occurred in some of the disomic substitution lines, but viral titers were significantly higher than those of two Lophopyrum species tested. This suggested that genes on more than one Lophopyrum chromosome are required for complete resistance to this virus. A potentially new gene for resistance to CYDV was detected on wheatgrass chromosome 3E. All of the substitution lines were susceptible to Mayetiola destructor and one strain of B. graminis. Disomic substitution lines containing wheatgrass chromosomes 1E and 6E were significantly more resistant to M. graminicola compared to Chinese Spring. Although neither chromosome by itself conferred resistance as high as that in the wheatgrass parent, they do appear to contain potentially new genes for resistance against this pathogen that could be useful for future plant-improvement programs.     

A demonstration of the inheritance of field resistance to lettuce downy mildew (Bremia lactucae Regel.) in progeny derived from cv. Grand Rapids

Summary The inheritance of field resistance to downy mildew in lettuce derived from cv. Grand Rapids was studied in F₂, F₃ and F₄ progeny from a cross with the more susceptible cv. Lobjoits Green Cos. The results for 32 randomly produced F₃ families and the F₂ population corresponded closely although the absolute level of resistance varied from season to season according to disease pressure and environmental conditions. The field resistance character showed a quantitative pattern of inheritance but the rapid response to selection at F₃ implied that only a few genes may be involved. F₄ families raised from families selected from the two extremes of the F₃ distribution fell into distinct categories with resistance levels corresponding to that of their F₃ parents. An association between resistance and morphological features of the resistant parent was present at F₂ but not in subsequent generations when individuals and families which were as resistant as cv. Grand Rapids and yet bore little morphological resemblance to this cultivar were readily recovered.     

Altered vegetation structure and composition linked to fire frequency and plant invasion in a biodiverse woodland

Relationships between fire history, vegetation structure and composition, and invasion by introduced plant species have received limited attention in Australian woodlands. A study in a Mediterranean, fire adapted urban Banksia woodland remnant in the biodiversity hotspot of southwest Australia investigated: (1) Have significant changes occurred in the woodland tree canopy between 1963 and 2000? (2) Do correlations exist between fire frequency and canopy cover? (3) If there is a difference in the vegetation composition of Banksia woodland invaded by the South African Ehrharta calycina (PCe) and Pelargonium capitatum (PCp) compared to largely intact remnants (GC)? and (4) Do correlations exist between vegetation condition, composition, fire frequency and invasion? Aerial photography, processed in a Geographical Information System, was used to establish fire history and changes in canopy cover over time (1963–2000). PCe and PCp sites experienced the greatest number of fires, with a net reduction in canopy cover in all areas experiencing four or more fires (60% of all woodlands). Frequent fire corresponded with a decline in native cover, richness and diversity, a shift from native to introduced species, changes in the relative importance of fire response categories, and loss of native resprouting shrub cover. Life forms of introduced species, which included no trees, shrubs and perennial sedges, contrasted strongly with those of native species, which had poor representation of annual and perennial grasses. Clear ecological and conservation consequences due to the loss of species diversity, changes in fire ecology and invasion have occurred in the Banksia woodlands. This study provides an understanding of the invasion process, enhancing conservation knowledge to improve the adaptive management of the key threatening process of invasion in biodiverse communities.     

Effects of Simulated Acid Mist on a Sitka Spruce Forest Approaching Canopy Closure: Significance of Acidified Versus Non-Acidified Nitrogen Inputs

Effects of enhanced N, S and NS Acid additions, up to approximately 100 kg N and S ha^?1 y^?1, are described for a 15-year-old Sitka spruce forest growing on an acid peat in Scotland. Groups of 10 trees, replicated over 4 blocks, have been treated at canopy height on approximately 50 or 100 occasions with 2 mm precipitation equivalent, between April and November, since 1996. Relative stem volume increment (RSVI) has been consistently higher in the NS Acid treated trees compared with control or N treated trees since the second year of treatment, although no dose response was found. Litterfall was also considerably increased in the NS Acid treatments and showed a clear dose effect but was not correlated with RSVI. Base cation concentrations in soil waters, collected using zero tension lysimeters reflected the presence or absence of the spray treatments and showed a dose related increase in response to NS Acid inputs. Treatment with 2NS Acid increased NH₄-N and NO₃-N by an order of magnitude. Results after 4 years of treatment showed a positive growth and litterfall response to NS Acid inputs but no effect of N alone. Enhanced stemwood growth may be linked to the higher base cation and phosphate concentrations measured in the soil water in the presence of NS Acid and S treatments.