高效阴离子交换色谱法测定毛头鬼伞多糖中的单糖组成

本文采用高效阴离子交换色谱—脉冲安培检测器（HAPEC-PAD），建立了一种测定多糖中单糖比例的方法。以NaOH为淋洗液、CarboPac^TM A20预处理柱，CarboPac^TMPA20分离柱，金工作电极，Ag/AgCl参比电极，8种自然界中常见单糖标准品做混合标样，探索方法可行性。在淋洗液浓度为2．5mmol／L时，各种单糖组分得到有效分离，其线性和重现性均良好并在此基础上测定了毛头鬼伞多糖中单糖比例；与传统的方法相比，此方法具有前处理简单、灵敏度高、节省时间和试剂等优点。     

几种抗生素对梨叶片愈伤组织和不定梢诱导的影响

研究了氨苄青霉素(Amp)、羧苄青霉素(Carb)、头孢霉素(Cef)和卡那霉素(Km)对梨叶片愈伤组织和不定梢诱导的影响。结果表明,Amp对巴梨叶片分化影响较小,当浓度达500mg/L时,其出愈率和不定梢再生率分别为96.48%和56.17%,与对照差异不显著;Cef浓度在50-300mg/L时对巴梨和身不知叶片再生均无显著影响,只有当Cef浓度高于400mg/L时,巴梨的不定梢再生率才受到显著抑制;巴梨和身不知叶片对Carb的反应不相同,低浓度(≤200mg/L)的Carb对巴梨的出愈率和不定梢再生率影响较小,却显著抑制身不知的不定梢再生率,高浓度(≥300mg/L)的Carb不仅极显著抑制巴梨和身不知的出愈率,而且完全抑制了2者的不定梢再生;Km浓度大于10mg/L时,明显抑制愈伤组织的生长,且随着浓度的增加,死亡率逐渐增大,当Km浓度达100mg/L时,巴梨和身不知叶片死亡率达100%和96.30%。     

不同化学物质打破葡萄、桃休眠的研究

以保护地栽培的葡萄、桃为试材,对氰氨类、细胞分裂素、赤霉酸和KNO3打破休眠的效果进行了研究。结果表明,1.5%单氰氨、11%石灰氮可明显促使葡萄提前萌芽,提早着色9-10d;50mg/LGA3和100mg/L6-BA对打破休眠基本无效。TDZ与单氰氨混用提高萌芽率的效果最好。在2个葡萄品种上,均没有观察到单氰氨或石灰氮处理对果穗或果实品质性状的不良影响。在华光油桃上,没有观察到化学物质对提早开花或成熟有明显的作用。对葡萄和桃上应用化学物质打破休眠的有关问题进行了讨论。     

日光温室内外金太阳杏光合特性的比较研究

为了探明杏对设施栽培的生理反应,对日光温室内外金太阳杏光合特性进行了比较研究。结果表明,日光温室栽培条件下,金太阳杏单位重量叶片的叶绿素含量增加;光(CO2)饱和点和光(CO2)补偿点降低鸦露地晴天净光合速率Pn日变化呈明显的双峰曲线,温室内也呈双峰线,但双峰出现原因不同。并且研究了影响光合作用的主要生态因子及变化规律,发现净光合速率与胞间CO2浓度、蒸腾作用、气温、叶温、光合有效辐射等因子相关。     

剡溪山水文化旅游资源开发设想

文化底蕴越是丰厚的旅游资源，开发价值就越高。正是众多文人贤士游剡访古，使浙东剡溪的历史、文化与自然山水完美地结合在一起，形成了绚丽多彩的剡溪山水文化。文化被认为是旅游业的灵魂，是一种潜在的旅游产品。要提高山水旅游产品的文化品位，必须将山水文化的潜在价值转化为旅游产品。本文对剡溪山水文化旅游资源的开发提出了以下设想：再现“唐诗之路”山水旅游美景，并着重于按唐诗及有关文献、传说等增添新景点，沿江、沿山、于水中进行山水、文化全面开发，形成立体式、全景式的剡中山水文化游道；可开发出文化生态、修学、科考、宗教、节庆等剡溪山水文化旅游产品。     

Malignant catarrhal fever-like disease in sheep after intranasal inoculation with ovine herpesvirus-2.

A malignant catarrhal fever (MCF)-like disease was induced experimentally in 3 sheep after aerosol inoculation with ovine herpesvirus-2 (OvHV-2). Each of 3 OvHV-2-negative sheep was nebulized with 2 ml of nasal secretions containing approximately 3.07 X 10(9) OvHV-2 DNA copies from a sheep experiencing an intensive viral-shedding episode. Ovine herpesvirus-2 DNA became detectable by polymerase chain reaction in the peripheral blood leukocytes of all 3 sheep within 3 days, and all 3 seroconverted between 6 and 8 days postinfection (PI). The sheep developed clinical signs, with copious mucopurulent nasal discharge and fever around 14 days PI. One of the 3 clinically affected sheep was euthanized at 18 days PI. Major lesions at necropsy were multifocal linear erosions and ulcers in mucosa of the cheeks, tongue, pharynx, and proximal esophagus and mild disseminated pneumonia. Microscopically, there was extensive moderate superficial histiocytic-lymphocytic rhinitis with epithelial dissociation and degeneration. Moderate multifocal histiocytic bronchointerstitial pneumonia was associated with loss of terminal bronchiolar epithelium. Lymphocytic vasculitis was present only in the lung. The remaining 2 sheep recovered clinically, approximately 25 days PI. The study revealed that clinical signs and lesions resembling MCF can develop when uninfected sheep are exposed to a high dose of aerosolized OvHV-2.     

中国香瓜与菜瓜地方品种资源白粉病抗性评价

春秋2个季节在温室利用风媒接种方法对搜集的102份香瓜和14份菜瓜种质资源进行了白粉病抗性评价,结果鉴定出7份香瓜和1份菜瓜高抗白粉病,其病情指数为零,1份菜瓜抗病,其病情指数0.9,表明我国香瓜和菜瓜地方品种资源中蕴涵着潜在的改良甜瓜白粉病抗性的基因资源。     

樟子松在西北干旱沙区的光合日变化特征

在甘肃省民勤治沙站 ,用 Li- 62 0 0便携式光合仪分别测定人工栽植的 4龄、9龄和 2 0龄樟子松的净光合速率日进程 ,其结果表明 :在干旱沙区 ,9龄以上樟子松的光合速率在午前随光强和气温而上升 ,午后下降。其 4龄、9龄和 2 0龄樟子松的最大净光合速率分别为 8.7μmol· m- 2 · s- 1、62 .3μmol· m- 2 · s- 1、1 9.7μmol· m- 2 · s- 1。     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro

AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.