Immunoferritin and immune electron microscopic study of bovine herpesvirus strain DN-599.

Intracellular antigens of strain DN-599 bovine herpesvirus were detected in the cytoplasm and the nucleus of infected bovine embryonic kidney cells by the indirect immunoferritin (IF) technique. Specific tagging was observed in viral envelope and capsids. Aggregates of viral particles heavily coated with antibody were seen by immune electron microscopy (IEM).     

Effect of the herbicide butachlor on methane emission and ebullition flux from a direct-seeded flooded rice field

 Application of a commercial formulation of the herbicide butachlor (N-butoxymethyl-2-chloro-2′,6′-diethyl acetanilide) at 1 kg a.i. ha^–1 to an alluvial soil planted with direct-seeded flooded rice (cv. Annada), significantly inhibited both crop-mediated emission and ebullition fluxes of methane (CH₄). Over a cropping period of 110 days, the crop-mediated cumulative emission flux of CH₄ was lowered by ∼20% in butachlor-treated field plots compared with that of an untreated control. Concurrently, ebollition flux of CH₄ was also retarded in butachlor-treated field plots by about 81% compared with that of control plots. Significant relationships existed between CH₄ emission and redox potential (E _h) and Fe²⁺ content of the flooded soil. Application of butachlor retarded a drop in soil redox potential as well as accumulation of Fe²⁺ in treated field plots. Methanogenic bacterial population, counted at the maturity stage of the crop, was also low in butachlor-treated plots, indicating both direct and indirect inhibitory effects of butachlor on methanogenic bacterial populations and their activity. Results indicate that butachlor, even at field-application level, can effectively abate CH₄ emission and ebollition from flooded soils planted to rice whilst maintaining grain yield. Received: 15 March 2000     

Water transmission characteristics of a Vertisol and water use efficiency of rainfed soybean (Glycine max (L.) Merr.) under subsoiling and manuring

In Vertisols of central India erratic rainfall and prevalence of drought during crop growth, low infiltration rates and the consequent ponding of water at the surface during the critical growth stages are suggested as possible reasons responsible for poor yields (<1 t ha⁻¹) of soybean (Glycine max (L.) Merr.). Ameliorative tillage practices particularly deep tillage (subsoiling with chisel plough) can improve the water storage of soil by facilitating infiltration, which may help in minimizing water stress in this type of soil. In a 3-year field experiment (2000–2002) carried out in a Vertisol during wet seasons at Bhopal, Madhya Pradesh, India, we determined infiltration rate, root length and mass densities, water use efficiency and productivity of rainfed soybean under three tillage treatments consisting of conventional tillage (two tillage by sweep cultivator for topsoil tillage) (S₁), conventional tillage + subsoiling in alternate years using chisel plough (S₂), and conventional tillage + subsoiling in every year (S₃) as main plot. The subplot consisted of three nutrient treatments, viz., 0% NPK (N₀), 100% NPK (N₁) and 100% NPK + farmyard manure (FYM) at 4 t ha⁻¹ (N₂). S₃ registered a significantly lower soil penetration resistance by 22%, 28% and 20%, respectively, at the 17.5, 24.5 and 31.5 cm depths over S₁ and the corresponding decrease over S₂ were 17%, 19% and 13%, respectively. Bulk density after 15 days of tillage operation was significantly low in subsurface (15–30 cm depth) in S₃ (1.39 mg m⁻³) followed by S₂ (1.41 mg m⁻³) and S₁ (1.58 mg m⁻³). Root length density (RLD) and root mass density (RMD) of soybean at 0–15 cm soil depth were greater following subsoiling in every year. S₃ recorded significantly greater RLD (1.04 cm cm⁻³) over S₂ (0.92 cm cm⁻³) and S₁ (0.65 cm cm⁻³) at 15–30 cm depth under this study. The basic infiltration rate was greater after subsoiling in every year (5.65 cm h⁻¹) in relation to conventional tillage (1.84 cm h⁻¹). Similar trend was also observed in water storage characteristics (0–90 cm depth) of the soil profile. The faster infiltration rate and water storage of the profile facilitated higher grain yield and enhanced water use efficiency for soybean under subsoiling than conventional tillage. S₃ registered significantly higher water use efficiency (17 kg ha⁻¹ cm⁻¹) over S₂ (16 kg ha⁻¹ cm⁻¹) and S₁ (14 kg ha⁻¹ cm⁻¹). On an average subsoiling recorded 20% higher grain yield of soybean over conventional tillage but the yield did not vary significantly due to S₃ and S₂. Combined application of 100% NPK and 4 t farmyard manure (FYM) ha⁻¹ in N₂ resulted in a larger RLD, RMD, grain yield and water use efficiency than N₁ or the control (N₀). N₂ registered significantly higher yield of soybean (1517 kg ha⁻¹) over purely inorganic (N₁) (1392 kg ha⁻¹) and control (N₀) (898 kg ha⁻¹). The study indicated that in Vertisols, enhanced productivity of soybean can be achieved by subsoiling in alternate years and integrated with the use of 100% NPK (30 kg N, 26 kg P and 25 kg K) and 4 t FYM ha⁻¹.     

Trace elemental characterization of some food crustacean tissue samples by EDXRF technique

The trace elemental analysis of two species of mud crab and three species of prawn samples from Chilika lagoon, Orissa, India has been carried out by using Energy Dispersive X-ray Fluorescence (EDXRF) technique available at Institute of Physics, Bhubaneswar. Elements namely K, Ca, Mn, Fe, Cu, Zn, Se, Br, Sr and Pb have been measured in the present investigation. The study indicates the effectiveness of the technique in analyzing biological materials like tissue samples and opens a door for easy analysis of seafood items with a easy, fast, sensitive, simultaneous multi-elemental technique with a simple sample preparation procedure without any chemical treatment. Though all the specimens were collected from the same environment of the lagoon the elemental variation might be due to the differential migration pattern and metabolism or other biological factors.     

Micronutrients (Fe,Mn, Zn and Cu) balance under long-term application of fertilizer and manure in a tropical rice-rice system

Purpose

The balance of micronutrients in soils is important in nutrient use efficiency, environmental protection and the sustainability of agro-ecological systems. The deficiency or excess of micronutrients in the plough layer may decrease crop yield and/or quality. Therefore, it is essential to maintain appropriate levels of micronutrients in soil, not only for satisfying plant needs in order to sustain agricultural production but also for preventing any potential build-up of certain nutrients.

Materials and methods

A long-term fertilizer experiment started in 1969 at Central Rice Research Institute, Cuttack, Odisha, India. Using this experiment, a study was conducted to analyze the balance of micronutrients and their interrelationship. The experiment was composed of ten nutrient management treatments viz. control; nitrogen (N); N + phosphorus (NP); N + potassium (NK); nitrogen, phosphorus and potassium (NPK); farmyard manure (FYM); N + FYM; NP + FYM; NK + FYM; and NPK + FYM with three replications. Micronutrients in soil (total and available), added fertilizers and organic manures and in rice plant were analyzed. Besides, atmospheric deposition of the micronutrients to the experimental site was also calculated. A micronutrient balance sheet was prepared by the difference between output and input of total micronutrients.

Results and discussion

Application of FYM alone or in combination with chemical fertilizer increased the diethylenetriamine pentaacetate (DTPA)-extractable Fe, Mn and Zn over the control treatment. The treatment with NPK + FYM had the highest soil DTPA-extractable Fe, Mn, Zn and Cu after 41 years of cropping and fertilization. Application of chemical fertilizers without P decreased the DTPA-extractable Zn over the control while the inclusion of P in the fertilizer treatments maintained it on a par with the control. The application of P fertilizer and FYM either alone or in combination significantly increased the contents of total Fe, Mn, Zn and Cu in soil mainly due to their micronutrient content and atmospheric depositions. A negative balance of Zn was observed in the N, NP, NK and NPK treatments, while a positive balance observed in the remaining treatments. The balance of Mn was negative in all the treatments, due to higher uptake by the rice crop than its addition.

Conclusions

Long-term application of chemical fertilizers together with FYM maintained the availability of micronutrients in soil and, thus, their uptake by rice crop.