Acaricide resistance in Rhipicephalus microplus collected from selected districts of Madhya Pradesh,Uttar Pradesh and Punjab states of India

Tropical Animal Health and Production - The isolates of Rhipicephalus microplus collected from Madhya Pradesh (MP), Punjab (PJB) and Uttar Pradesh (UP) states of India were characterized using...     

Canine periodontal disease control using a clindamycin hydrochloride gel

Stabilizing or reducing periodontal pocket depth can have a positive influence on the retention of teeth in dogs. A topical 2% clindamycin hydrochloride gel (CHgel) was evaluated for the treatment of periodontal disease in dogs. The CHgel formulation provides for the sustained erosion of the matrix, but also flows into the periodontal pocket as a viscous liquid, and then rapidly forms a gel that has mucoadhesive properties and also may function as a physical barrier to the introduction of bacteria. A professional teeth cleaning procedure including scaling and root planing was done in dogs with one group receiving CHgel following treatment. Periodontal health was determined before and after the procedure including measurement of periodontal pocket depth, gingival index, gingival bleeding sites, and number of suppurating sites. There was a statistically significant decrease in periodontal pocket depth (19%), gingival index (16%), and the number of bleeding sites (64%) at 90-days in dogs receiving CHgel. Additionally, the number of suppurating sites was lower (93%) at 90-days for the group receiving CHgel. The addition of CHgel effectively controlled the bacterial burden (e.g, Fusobacterium nucleatum) at both day 14 and 90. Gingival cells in culture were shown to rapidly incorporate clindamycin and attain saturation in approximately 20-minutes. In summary, a professional teeth cleaning procedure including root planning and the addition of CHgel improves the gingival index and reduces periodontal pocket depth.     

Growth Analysis Using Classical and Functional Techniques in Relation to Soil Moisture in Mustard

A Reid experiment was conducted to study the effect of soil moisture on growth of two mustard cultivars using classical and functional techniques of growth analysis. Two soil moisture levels were irrigated at 10-day intervals and a rainfed control was included. Total dry matter (TDM), leaf area index (LAI) and leaf area duration (LAD) were significantly increased by irrigation at most of stages of growth. Starting from a lower value, LAI and LAD reached a peak and then gradually declined. Among the growth attributes, crop growth rate (CGR), leaf area ratio (LAR) and leaf weight ratio (LWR) increased significantly under irrigation. Net assimilation rate (NAR) decreased more in the irrigated plants than the rainfed plants at the later stages of growth. LAR and LWR declined throughout with increasing time and plant weight. Seed yield of the irrigated plants was positively correlated with the pre-flowering LAI and the post-flowering CGR and NAR. In the rainfed plants, seed yield was positively correlated with LAI and CGR at the post-flowering stage and negatively with the post-flowering NAR and pre-flowering LAR.     

Genetic Variance Estimates in Heterogeneous Potato Populations Propagated from True Potato Seed(TPS)

Twenty nine cross populations developed according to line x tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ^2p was mostly contributed by the δ^2g. Among the seven characters, tuber weight per plant showed the highest δ^2g, PCV, GCV, H2b, GA and expected GA%.On the other hand, dry matter contents (%) showed the lowest H^2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive.     

Liquid chromatography-electrospray ionization-tandem mass spectrometry method for the determination of epoxidized soybean oil in food products

Epoxidized soybean oil (ESBO) is widely used as a plasticizer and stabilizer in such polymers [poly(vinyl chloride) in particular] commonly adopted for manufacturing of gaskets of the lids for glass jars and plastic films for food packaging. Human exposure to ESBO and its derivatives is likely to occur over a lifetime with a significant variation according to life stage. A reversed phase liquid chromatography interfaced with electrospray ion trap tandem mass spectrometry method for the determination of ESBO in foods was developed. A simple sample treatment procedure entailing the use of an extraction step with dichloromethane without any further cleanup was proved. Chromatographic separation was performed using two C18 columns with an aqueous acetic acid-acetone-acetonitrile mixture as the mobile phase under gradient conditions. The method was validated in terms of detection limits (4 mg kg(-1)), quantitation limits, linearity (established over 2 orders of magnitude), recovery (good mean recoveries, higher than 90% for all of the signals detected), precision (RSD% < 8), and trueness. The applicability of the method to the determination of ESBO in different food matrices (in particular those rich in edible oil) was demonstrated, and the performances were compared to those reachable by the commonly well-known gas chromatography-mass spectrometry procedure.     

Selection of Suitable Parents in the Development of Potato Hybrids in Bangladesh

Potatoes(Solanum tuberosum L)are the thirdlargest food crop in Bangladesh following rice andwheat and make a major contribution to the total foodsupply of the country.Although in many westerncountries potato is the number one staple food,it is al-most entirely used as a vegetable in Bangladesh.Thepotato is a short duration crop that produces a largeamount of calories in a short period of time[1].Thepotato produces more protein and calories per unitarea per unit time than any other major food…     

An alternative eco-friendly approach for sustainable crop production with the use of indigenous inputs under old alluvial soil zone of Burdwan,West Bengal,India

Experiments were conducted on mustard (Brassica campestris cv.B₉) in an old alluvial soil zone of Crop Research and Seed Multiplication Farm, Burdwan University, Burdwan, West Bengal, India, during the winter seasons of 2011–2012 and 2012–2013. The aim was to evaluate the use of vermicompost prepared from municipality waste and Eichhornia mixture and its efficacy on crop growth and yield. Different combined doses of vermicompost, dried cow dung and chemical fertilizer along with Azotobacter and phosphate-solubilizing bacteria compared to full recommended dose of chemical NPK fertilizer (100:50:50) were used to develop an alternative farming technology for sustainable crop production and conservation of natural resources. The variety B₉ gave a significantly higher seed yield and oil content along with other growth and yield-contributing factors as well as being the most economically viable option against treatment T4 (i.e. 75% NPK + vermicompost at 2.5 tons per hectare) among all the treatments applied for the experiment and was found to be superior to other treatments in old alluvial soil of Burdwan, India. In both the experimental years, seed yield and oil content were found to be the best for the treatment T4 and was better than using chemical fertilizer.     

Potassium fractions with other nutrients in crops: A review focusing on the tropics

Potassium (K), a plant nutrient with diverse roles to play in plant metabolism, is required in large amounts by most crops. It interacts with many other plant constituents to affect crop yield and quality. The magnitude of this interaction is high in areas of high cropping intensity, as in the tropics. The interaction of nutrients with K may be in the soil or in plant. Potassium modifies ammonium (NH₄ ⁺) ion fixation in soils to restrict nitrogen (N) availability. On the other hand, an antagonistic effect between K and NH₄ absorption has been suggested in which K absorption is restricted. Similarly, magnesium (Mg) or calcium (Ca) deficiency occurs from ion antagonism in acid soils following K fertilization and in soils with high exchangeable K. Sulfur (S) has been reported to increase K absorption and productivity of oilseed crops. With increasing levels of applied or soil K, the severity of phosphorus (P)‐induced zinc (Zn) deficiency in corn has been observed to decrease. Application of K decreases manganese (Mn) content and iron (Fe) toxicity in rice. Application of K has been reported to decrease B levels in plants and to increase incidence of boron (B) deficiency. Top‐dressing with K fertilizer was reported to lower the copper (Cu) content of alfalfa forage. In root, sugar‐producing, or fiber‐producing crops, the sodium (Na) and K relationship is important with the specific response to either element depending on which element is in low or high supply. Molybdenum (Mo) stimulated K uptake in alfalfa and com. In intensive agriculture with high‐yielding single crops or with multiple crops per year, farm management must include strategies to maintain substantial K reserves in the soil and to balance K nutrition with other fertilization practices.     

QTL for yield, yield components and canopy temperature depression in wheat under late sown field conditions

A wheat (Triticum aestivum L.) recombinant inbred line (RIL) population was used to identify quantitative trait loci (QTL) associated with yield, yield components, and canopy temperature depression (CTD) under field conditions. The RIL population, consisting of 118 lines derived from a cross between the stress tolerant cultivar ‘Halberd’ and heat stress sensitive cultivar ‘Karl92’, was grown under optimal and late sown conditions to impose heat stress. Yield and yield components including biomass, spikes m^?2, thousand kernel weight, kernel weight and kernel number per spike, as well as single kernel characteristics were determined. In addition, CTD was measured during both moderate (32–33 °C) and extreme heat stress (36–37 °C) during grain-filling. Yield traits showed moderate to high heritability across environments with a large percentage of the variance explained by genetic effects. Composite interval mapping detected 25 stable QTL for the 15 traits measured, with the amount of phenotypic variation explained by individual QTL ranging from 3.5 to 27.1 %. Two QTL for both yield and CTD were co-localized on chromosomes 3BL and 5DL and were independent of phenological QTL. At both loci, the allele from Halberd was associated with both higher yield and a cooler crop canopy. The QTL on 3BL was also pleiotropic for biomass, spikes m^?2, and heat susceptibility index. This region as well as other QTL identified in this study may serve as potential targets for fine mapping and marker assisted selection for improving yield potential and stress adaptation of wheat.