Relation of soya bean meal level to the concentration of plasma free amino acids and body growth in white rats

Amino acid (AA) levels in plasma and body growth were determined in rats (n20) fed diets with different soya bean meal levels. Free AA in plasma was determined by reversed-phase high-pressure liquid chromatography. We have used four levels of protein diets like 8%, 15%, 23% and 35% in this trial. Rats which were fed the low-protein (8%) diet with low percentage of soya bean meal were found to be growth-retarded. The body weight gain of high protein group (35%) was lower than that of the 23% groups. In the rats fed with the low-soya bean meal diet, some nonessential AA (NEAA) in plasma like asparagine, aspartic acid, cysteine, glutamic acid and serine increased, whereas the essential AA (EAA), with the exception of arginine, methionine and valine decreased. Here, plasma EAA-to-NEAA ratios were not correlated to growth and experimental diet. We hypothesize that AA metabolism is associated to changes in growth in rats on different protein intake. This study has showed the sensitivity of body mass gain, feed intake, feed conversion rate of rats to four levels of protein in the diet under controlled experimental conditions.     

Multi-polarized Radarsat-2 satellite sensor in assessing forest vigor from above ground biomass

Accurate estimates of forest biomass are increasingly important in relation to sequestration of carbon by forest trees.Satellite remote sensing is a useful tool for biomass estimation and monitoring of forest ecological processes.Microwave synthetic aperture radar(SAR) can increase the accuracy of estimations of forest biomass in comparison to optical remote sensing, due to the unique capacities of SAR, including high penetrability, volumetric scattering, interaction with surface roughness, and dielectric property.We studied the potential of multi-polarized C-band Radarsat-2, a SAR technology, with HH, HV and VV polarization for estimating biomass of moist tropical Indian forest.Backscatter values are correlated with fieldbased biomass values and are regressed to generate models for estimating biomass.HH polarization provided maximum information regarding tree biomass.A coefficient of determination of 0.49 was calculated for HH polarized C-band image with in situ measurements.An exponential model was proved to be best suited for estimating forest biomass.Correlation of 0.62 and RMSE of 24.6 t ha~(-1) were calculated for the relationship between estimated and predicted biomass values for the best fit model.The average absolute accuracy of the model was 61%, while Willmott's index of agreement was 0.87.Results suggest that most of the biomass of the area ranged within70 t ha~(-1) a probably due to the saturation of C-band around 60–70 t ha~(-1) for tropical forests.     

Arsenic contamination in cropping systems under varying irrigation sources in the deltaic plain of India

A field experiment was conducted in an arsenic endemic area of West Bengal, India (22°57?N, 89°33?E) in 2010–2012 to understand different prevalent cropping systems of the area as to nature of arsenic uptake by the crops and influence of different sources of irrigation water. The experiment was laid out in split plot design consisting two irrigation managements [I₁: irrigation with shallow tube well (STW) and I₂: irrigation from harvested pond water (PW)] in main plot and four cropping systems in sub plot were C₁: pea- summer rice- cowpea, C₂: potato- green gram- elephant foot yam (EFY), C₃: wheat- jute- winter rice and C₄: French bean- sesame- winter rice. Irrigation from PW recorded less arsenic uptake compared to STW. Arsenic uptake was minimum with French bean- sesame- winter rice (C₄), followed by potato – green gram – EFY (C₂). System equivalent yield was the highest with C₂. The highest return was recorded with C₂ and the return per dollar (USD) investment was the maximum with C₁, followed by C₂. Potato- green gram- EFY (C₂) proved to be the better option for the farmers in arsenic contaminated area with greater yield potential, highest return per dollar investment and less arsenic uptake.     

Maternal antibody to infectious bronchitis virus: its role in protection against infection and development of active immunity to vaccine

Chicks hatched with high levels of maternal antibody had excellent protection (>95%) against infectious bronchitis virus (IBV) challenge at 1 day of age, but not at 7 days (<30%). This protection significantly (P<0.05) correlated with levels of local respiratory antibody and not with serum antibody.A high percentage of both maternal antibody-positive (Mab+) and maternal antibody-negative (Mab-) chicks failed to produce IBV antibody when vaccinated at 1 day of age by the intraocular route. In addition, Mab+ chickens had a weaker virus-neutralizing antibody response to a second IBV vaccination compared to Mab- birds (P<0.05). Mab+ chicks experienced a more rapid decline (P<0.01) in maternal antibody after 1-day-of-age vaccination compared to their unvaccinated counterparts.A monoclonal antibody-based blocking ELISA that measured antibody levels specific to S1 glycoprotein of IBV correlated well with virus-neutralizing antibody titers.     

Establishment of persistent avian infectious bronchitis virus infection in antibody-free and antibody-positive chickens

Avian infectious bronchitis virus (IBV) causes a highly contagious and economically significant disease in chickens. Establishment of a carrier state in IBV infection and the potential for the persistent virus to undergo mutations and recombination in chicken tissues have important consequences for disease management. Nevertheless, whether chickens can maintain persistent IBV infection in the absence of reinfection from exogenous sources or the presence of antibody in the host can modulate virus persistence remains unclear. Indeed, whether or not IBV genome can undergo genetic changes during in vivo infection has not been demonstrated experimentally. In the present study, IBV shedding and tissue persistence were monitored in individual chickens maintained under strict isolation that precluded reinfection from exogenous sources. In the first of two experiments, intranasal exposure of 6-wk-old antibody-free chickens to IBV vaccine virus resulted in intermittent shedding of the virus from both trachea and cloaca of individual birds for up to 63 days. Also, the virus was recovered from the internal organs (spleen, gonad, kidney, lung, cecal tonsil, and cloacal bursa) of six of eight birds killed at various intervals between 27 and 163 days postinoculation (DPI). In the second experiment, IBV exposure of 1-day-old maternal antibody-positive chicks led to periodic virus shedding from the trachea and cloaca in all chickens until 77 days; however, internal organs (lungs and kidneys) of only one of seven birds (killed at 175 DPI) were virus positive, suggesting that presence of antibody at the time of infection protects internal organs from IBV infection. When the lung and kidney isolates of IBV from the latter experiment were compared with the parent-vaccine virus, no changes in their antigenicity, tissue tropism, or the nucleotide sequence of the S1 glycoprotein gene were observed. These findings indicate that, unlike the mammalian coronaviruses, propensity for frequent genetic change may not be inherent in the IBV genome.     

Characterization of infectious bronchitis virus isolates by slot blot hybridization

We used slot blot hybridization of the hypervariable regions of the S1 subunit of spike peplomer gene to identify and characterize infectious bronchitis virus (IBV) strains. Template DNA was created from six reference strain IBVs of different serotypes and immobilized on a nitrocellulose membrane. We synthesized digoxigenin-labeled probes from reference and unknown field viruses and hybridized them to template DNA. All reference strains could be distinguished and isolates identified by serotype if they were at least 95% identical to a reference strain. This slot blot hybridization procedure was specific and reproducible, and strain typing was consistent with the S1 sequencing of the IBV genome. This study thus provides a simple and rapid method for typing of IBV.     

Diversity,structure and standing stock of wood in the homegardens of Kerala in peninsular India

A survey was conducted in 17 selectedthaluks (revenue sub-divisions) of Kerala state to elucidate the floristic structure, composition and the extent of similarities and diversities in the composition of homegardens of Kerala, with particular reference to small (below 0.4 ha), medium (0.5 to 2 ha) and large (>2.0 ha) holding size categories. Besides attempts wer also made to characterize the potential of homegardens to supply commercial timber and fuelwood. Two hundred and fifty two farmers were selected through a stratified random process. In addition to gathering general information on crop and livestock production enterprises, all scattered trees and shrubs (15 cm girth at breast height) on the homestead and border trees except palms and rubber were enumerated.There was tremendous variability both in the number of trees and shrubs present and species diversity of the selected homesteads in different provinces. The small, medium and large sized holdings also exhibited profound variability in the number of woody taxa and individuals present. In total, 127 woody species (Girth at Breast Height (GBH) 15 cm) were encountered. The mean number of woody taxa found in the homegardens ranged from 11 for Pathanapuram to 39 in Perinthalmanna. Floristic diversity was higher in the smaller homesteads. It decreased with increasing the size of holdings. Mean Simpson's diversity index for the homesteads ranged from 0.251 (Kochi) to 0.739 (Kottarakkara) suggesting that floristic diversity of homegardens was moderate to low compared to a value over 0.90 for the species-rich evergreen forests of the Western Ghats. The Sorensen's similarity indexes suggested a moderately high degree of similarity for the different tree species encountered in the homesteads of Kerala.No clear cut planting pattern was discernible in the homegardens of Kerala. The homegarden trees and shrubs were either scattered throughout the homestead or on farm boundaries. Farmers tend to prefer timber trees such as ailanthus (highest frequency) and teak besides fruit trees such as mango, jack, cashew and the like. Major homegarden species were represented in all diameter classes. The diameter structure, however, exhibited a slightly skewed (+) distribution pattern, having the highest frequency in the 20–30 cm classes ensuring adequate regeneration status and in that process making homegardens a sustainable as well as dynamic land use system.Standing stock of timber and firewood in the homestead of differentthaluks are presented. Average commercial standing stock of homesteads ranged from 6.6 to 50.8 m³ ha^–1 and fuel wood volume was of the order of 23 to 86 m³ ha^–1. Implicit in the high commercial timber volume and fuel wood volume is that a substantial proportion of the society's wood demands are met from the homesteads. Palms, however, constituted the dominant component of standing commercial timber and fuel wood volumes accounting as much as 63% and 72%, respectively, of the total wood in these categories.