全文获取类型
收费全文 | 16496篇 |
免费 | 5篇 |
专业分类
林业 | 3633篇 |
农学 | 1294篇 |
基础科学 | 137篇 |
2745篇 | |
综合类 | 715篇 |
农作物 | 2095篇 |
水产渔业 | 1793篇 |
畜牧兽医 | 1114篇 |
园艺 | 1111篇 |
植物保护 | 1864篇 |
出版年
2022年 | 3篇 |
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 2747篇 |
2017年 | 2702篇 |
2016年 | 1179篇 |
2015年 | 67篇 |
2014年 | 16篇 |
2013年 | 16篇 |
2012年 | 793篇 |
2011年 | 2130篇 |
2010年 | 2103篇 |
2009年 | 1259篇 |
2008年 | 1317篇 |
2007年 | 1576篇 |
2006年 | 35篇 |
2005年 | 101篇 |
2004年 | 104篇 |
2003年 | 153篇 |
2002年 | 62篇 |
2001年 | 7篇 |
2000年 | 47篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 14篇 |
1992年 | 8篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 9篇 |
1988年 | 12篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1977年 | 4篇 |
1972年 | 1篇 |
1969年 | 2篇 |
1968年 | 5篇 |
1967年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
52.
A double seismic zone for deep earthquakes was found in the Izu-Bonin region. An analysis of SP-converted phases confirms that the deep seismic zone consists of two layers separated by approximately 20 kilometers. Numerical modeling of the thermal structure implies that the hypocenters are located along isotherms of 500 degrees to 550 degrees C, which is consistent with the hypothesis that deep earthquakes result from the phase transition of metastable olivine to a high-pressure phase in the subducting slab. 相似文献
53.
S. Gandhi Doss S. P. Chakraborti S. Roychowdhuri N. K. Das K. Vijayan P. D. Ghosh 《Euphytica》2012,185(2):215-225
Sericulture is an agro-based industry, which heavily depends on mulberry (Morus spp.) for its sustainability, as mulberry leaf is the only available feed for the silkworm Bombyx mori L. Sericulture, in West Bengal and other subtropical regions in India, suffers from lack of adequate quantity of quality
mulberry leaf during the colder months, the best season for rearing the high yielding bivoltine silkworm hybrids. In order
to develop mulberry varieties, which can sustain normal growth during these colder months to yield better, efforts have been
made in different research institutes in India. The present report is part of such an effort in which accessions in germplasm
bank were screened for better growth, leaf retention and leaf yield contributing associated traits. Twenty three selected
parents were crossed, 3,500 seeds were sown, 2,700 seedlings were transplanted to progeny row trail, selected 210 hybrids
were evaluated under primary yield trial and finally nine hybrids along with a control variety were studied in detail for
all leaf yield contributing traits under final yield trial. Stability analysis was adopted to identify hybrids, which can
yield stably across seasons. The hybrids CT-44 and CT-11 out yielding the control by 17.17 and 7.11% were selected as these
hybrids yield 7.93 and 8.15 mt/ha leaf respectively during the colder months (February) for their direct use as cultivars
in West Bengal and other subtropical areas of India to sustain bivoltine sericulture to produce quality and gradable silk
fibers. 相似文献
54.
Solidity of microsatellite markers is a key issue for varietal identification, especially when they are used for legal purposes, what includes their probable future use in the distinctness, uniformity and stability testing of new varieties needed for the granting of Plant Breeders’ Rights. Nine grapevine microsatellites (VVS2, VVMD5, VVMD27, VVMD28, ssrVrZAG29, ssrVrZAG62, ssrVrZAG67, ssrVrZAG83 and ssrVrZAG112), which had previously demonstrated its capacity to discriminate any grapevine variety, have been assessed to evaluate its uniformity and stability. 19 varieties were selected, representative of a high diversity for morphological, agronomical, cultural and historical aspects, as well as for microsatellite allele variability. Then, for each variety, uniformity and stability were evaluated through the analysis of 50 plants from each of three different plots, and five plants from each of seven additional plots. Material from 4,137 plants of 229 plots of the 19 varieties was sampled in seven countries. Of 3,654 plants analyzed with the set of nine microsatellites, 3,299 were of the right variety and used for the survey. An average of 172 individual values was studied for each allele of each microsatellite of each variety, and none differences were detected that could not be explained as technical variations, with the exception of several putative chimeras in two varieties. Of the total of 171 variety x microsatellite combinations, only in one combination (‘Merlot’ x VVMD27) the number of off-types exceeded the threshold allowed. The remaining 170 combinations have been found uniform and stable according to internationally accepted rules. 相似文献
55.
Moytri RoyChowdhury Yulin Jia Aaron Jackson Melissa H. Jia Robert Fjellstrom Richard D. Cartwright 《Euphytica》2012,184(1):35-46
The Pi-z gene in rice confers resistance to a wide range of races of the rice blast fungus, Magnaporthe oryzae. The objective of this study was to characterize Pi-z in 111 rice germplasm accessions using DNA markers and pathogenicity assays. The existence of Pi-z in rice germplasm was detected by using four simple sequence repeat (SSR) markers (RM527, AP4791, AP5659-1, AP5659-5) closely
linked to Pi-z, and was verified using pathogenicity assays with an avirulent strain (IE1k) and two virulent races (IB33 and IB49). Among
111 germplasm accessions evaluated, 73 were found to contain the Pi-z gene using both SSR markers and pathogenicity assays. The remaining 38 germplasm accessions were found to be inconsistent
in their responses to the blast races IB33, IEIk and IB49 with expected SSR marker alleles, suggesting the presence of unexpected
SSR alleles and additional R gene(s). These characterized germplasm can be used for genetic studies and marker-assisted breeding for improving blast resistance
in rice. 相似文献
56.
Zhi-Wei Wang Lei Gao Hai Zhou Liu Shi Yong Mei Yuan Zhou Chang Ping Xiang Ting Wang 《Euphytica》2012,186(2):313-320
A male sterile plant appeared in the radish breeding program at the Hubei Academy of Agricultural Sciences, Hubei, China.
In its progeny, a two-type (half of plants male sterile, the other half male fertile) line 01GAB was established. An F2 population of 260 plants from a cross of male-sterile 01GAB and a male fertile line 9802H segregated for male fertility in
a 3:1 ratio indicating that fertility was restored by a single dominant gene, here designated RsMs. A PCR-based DNA marker specific to the male fertility Rfob gene in 9802H was absent in 01GAB. Linkage analysis placed the RsMs locus 10.7 cM away from the Rfo locus. In an F2 population of hybrids between 01GAB and male fertile 9802B, a co-dominant DNA marker for the RSultr3.2A (a radish sulfate transporter gene) locus was linked to the RsMs locus at 1.5 cM suggesting that fertility restoration in 01GAB was located in the region with known male sterility restorers
in radish. However, no maintainer for the 01GAB source of male sterility has been identified so far. Cytological observations
have shown that the abnormalities in male sterile anthers first appeared in tapetum at the tetrad stage, followed by a hypertrophy
of the tapetal cells at the vacuolate microspore period. These results suggest that male sterility in 01GAB is likely to be
genetic in nature, or it may represent a new type of the cytoplasmic male sterility. 相似文献
57.
Rishi R. Burlakoti Mohamed Mergoum Shahryar F. Kianian Tika B. Adhikari 《Euphytica》2010,172(2):197-205
Fusarium head blight (FHB) is a devastating disease in wheat throughout the world. FHB resistance consists of two components: resistance
to initial infection (type I) and resistance to spread within infected spikes (type II). Current wheat breeding programs for
FHB focus on type II resistance, which limits pathogen spread but may not be sufficiently durable. To combine type I with
existing type II resistance, 113 F9-derived recombinant inbred lines (RILs) were developed from a cross between three wheat genotypes Frontana, W9207, and Alsen.
The RILs were evaluated for resistance to initial infection, FHB spread within spike, kernel damage, and deoxynivalenol (DON)
content in two independent greenhouse experiments in 2006 and 2007. Among the 113 RILs, 20% lines showed ≤10% initial disease
severity (IDS) and ≤11 to 30% final disease severity (FDS), and 19% had DON content ≤5 μg/g. Approximately 11% of the RILs
showed tendency of higher resistance (as exhibited by lower IDS, FDS, and DON content) than the resistant parents. The 42
of the FHB-resistant RILs were analyzed with seven simple sequence repeat (SSR) markers or microsatellites known to be linked
to FHB resistance. Approximately half of the RILs had molecular markers linked to both types of FHB resistance indicated
the presence of type I and II resistance alleles in the RILs. The resistant RILs identified in this study should be useful
for the future improvement of FHB resistance in spring wheat. 相似文献
58.
Aneeta Pradhan Julie A. Plummer Matthew N. Nelson Wallace A. Cowling Guijun Yan 《Euphytica》2010,176(1):87-98
A triploid hybrid with an ABC genome constitution, produced from an interspecific cross between Brassica napus (AACC genome) and B. nigra (BB genome), was used as source material for chromosome doubling. Two approaches were undertaken for the production of hexaploids:
firstly, by self-pollination and open-pollination of the triploid hybrid; and secondly, by application of colchicine to axillary
meristems of triploid plants. Sixteen seeds were harvested from triploid plants and two seedlings were confirmed to be hexaploids
with 54 chromosomes. Pollen viability increased from 13% in triploids to a maximum of 49% in hexaploids. Petal length increased
from 1.3 cm (triploid) to 1.9 cm and 1.8 cm in the two hexaploids and longest stamen length increased from 0.9 cm (triploid)
to 1.1 cm in the hexaploids. Pollen grains were longer in hexaploids (43.7 and 46.3 μm) compared to the triploid (25.4 μm).
A few aneuploid offsprings were also observed, with chromosome number ranging from 34 to 48. This study shows that trigenomic
hexaploids can be produced in Brassica through interspecific hybridisation of B. napus and B. nigra followed by colchicine treatment. 相似文献
59.
Jundae Lee Jae Bok Yoon Jung-Heon Han Won Phil Lee Sang Hoon Kim Hyo Guen Park 《Euphytica》2010,173(1):55-61
Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms
3
gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP)
techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms
3
locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and
flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using
the GMS system. 相似文献
60.
The objective of this study was to identify molecular markers linked to fruit-related traits in the tomato subjected to high
temperatures. In total, 160 F2 plants derived from a cross between a heat-tolerant breeding line, CL5915-93D4-1-0-3 (Solanum esculentum), and a heat-sensitive wild accession, L4422 (S. pimpinellifolium), were grown in a greenhouse. Six traits including fruit number, fruit weight, brix, seed number, fruit setting, and flower
number were scored. The distributions of fruit number, fruit set, flower number, and seed number were skewed towards heat
susceptibility which is known to be characteristic of L4422. Polymorphic bands were generated by PCR-derived methods of RAPD,
ISSR and AFLP Polymorphism, the segregation ratio, and distribution over the genome of the above 3 markers were compared.
Ten linkage groups, ranging 20.6–151.6 cM in size, were constructed with 62 informative markers spanning a total of 776.3 cM.
Fruit-related quantitative trait loci (QTLs) were non-randomly distributed in the tomato genome. For the 6 traits investigated,
21 QTLs were dispersed on linkage groups 2–5. The genetic effects of the various QTLs were differently exhibited, in our study
we have respectively found from 10.5% to 30.2% of the variation explained by the QTL for flower number (FRN4) and brix (BX2).
Thirteen QTL-mapped markers were unique to 1 trait, and 4 markers were linked to more than 1 trait. Among them, QTLs linked
to the I868-470 marker had effects on fruit weight and brix, and a significant positive correlation between these 2 traits
was noted (r = 0.35, P < 0.05). Thus, the I868-470 marker may have the potential for simultaneous selection of high fruit weight and brix. These
markers also allowed us to align genome linkage maps across distantly related species and to reveal the co-localization between
these QTLs and major genes. 相似文献