高效液相色谱(High-performance liquid chromaography)测定水产品中孔雀石绿及其代谢物的残留量的检测方法

本中心应用《水产品中孔雀石绿残留量的测定液相色谱法》（SC／T3021—2004）检测方法，多次对实际水产品中孔雀石绿及其代谢物的残留量进行检测，总结了一些经验并对方法中的一些环节进行了改进，使检测方法更加科学，回收率更合理，符合要求，检出限更低。     

马尾松材脱脂干燥的初步研究

马尾松(Pinus massoniana Lamb)材因有翘曲、变形、松脂多等缺陷,在使用上受到一定限制。作者进行的试验结果表明:通过一定温度、一定浓度碱溶液处理,进行脱脂干燥,可以提高材质,从而能够以松代杉,扩大利用范围,增加经济收益。     

乏情母猪治疗的试验

用PMSG +LH、维生素D3,维生素E对乏情母猪进行试验处理 ,结果表明 ,PMSG +LH、维生素D3,维生素E联合使用 ,效果最好     

珍稀濒危树种连香树造林试验

本文通过对连香树造林成活率、中幼龄林生长情况调查,结果表明,连香树在前期生长较快,病虫害少,树体大小中等,适宜作为城市行道树和园林、庭院绿化树种,影响栽植成活率和生长量的主导因子是水分,苗期和幼树易发生日灼危害,平原地区栽植应以4～5年生的大苗为主,栽植幼苗应适当遮荫。     

Cryptic occurrence of <Emphasis Type="Italic">Chattonella marina</Emphasis> var. <Emphasis Type="Italic">marina</Emphasis> in mangrove sediments in Probolinggo,East Java Province,Indonesia

Mangrove forests and adjacent creeks are known to be highly productive estuaries, which are partly supported by benthic microalgae that grow on the sediments. During surveys investigating the microalgal floras of mangrove swamps in the eastern part of Java Island, a mud sample unexpectedly included large numbers of a notorious fish killer, Chattonella-like motile cells, and its resting cysts. These motile cells were established as clonal cultures for further identification and physiological tests. The cysts were examined through palynological and molecular biological means. Identification based on light microscopy and ribosomal RNA gene sequences confirmed that these cells and cysts were Chattonella marina var. marina. While the strains were genetically identical to the temperate strains isolated from Japan and China, temperature experiments showed that the Indonesian strains possessed a high maximum quantum yield of photosystem II even after exposure to 34 °C, a temperature at which the Japanese strain could not survive. Salinity experiments showed adaptation of the strains to a salinity of 15. These findings, together with the discovery of populations of cysts in the mangrove sediment, highlight the tough and unique nature of the Indonesian strains, which are likely adapted to wide fluctuations of temperature and salinity in mangrove swamps, and pose a potential risk to fisheries in Indonesia.     

有益菌根真菌及其互作对带叶兜兰试管苗生理生长的影响

为探讨菌根真菌的互作效应,筛选协同互作模式组合,对从野生兜兰新鲜营养根中分离、筛选出的3种菌根真菌（PF02、PF06和PF07）,采用单菌接种、双菌混合接种和三菌混合菌种等7种接种方式,开展菌−苗共生培养,筛选出优势菌株PF02和PF07,揭示在实验室环境下接菌方式与接种效应之间的相互联系,分析菌根真菌互作对带叶兜兰植株生长和生理的影响。结果表明：显示菌根真菌互作对宿主植株的生物量、保护酶活性及叶绿素总量具有较好的正向效应。3种双菌混合接种方式PF02−PF06、PF02−PF07及PF06−PF07均能有效促进带叶兜兰生物量增长,尤其是PF02−PF06接种方式,抗氧化酶POD、超氧化物歧化酶SOD、过氧化氢酶CAT活性及叶绿素总量提高显著。选择优化的接种方式,以及能够协同促进、达到共生平衡的最佳接种方式组合,最大限度发挥菌根对宿主植物的益生作用。     

Effects of nitrogen and water stress on the rehydration,endogenous hormonal regulation and yield of maize

Water scarcity is known to be a strong limiting factor affecting maize grown and yield in cold semi-arid regions. Numerous studies have shown that rehydration improves maize growth. Our study aimed to explore the effects of rehydration treatments on maize growth and yield under water and nitrogen stress during different growth stages. We selected the drought-tolerant maize variety Nendan 19 (ND19) and subjected it to water stress during the V6 (sixth-leaf), R2 (filling) and R6 (maturity) growth stages and a rehydration treatment after each stress stage. Our results indicated that N1 (N100 kg N ha⁻¹) and N3 (N300 kg N ha⁻¹) treatments significantly increased the leaf moisture status relative to water content (RWC), bound water content (BWC), free water content (FWC) and water potential (WP)) at different growth stages. Similar trends were observed in the accumulation of plant leaf and root hormones (zeatin+zeatin riboside, indole-3-acetic acid, abscisic acid and gibberellic acid), photosynthetic pigments and chlorophyll fluorescence. However, under the same water stress conditions, they decreased as the N rate increased and reached a minimum value in the S3 (water stress for N3) treatments. In addition, with growth stage advancement and extension of the rehydration time, both showed a gradual upward trend. The results showed that to save water resources in the cold semi-arid region, rehydration treatments (R2S1 and R2S3) significantly increased the photosynthetic pigments and chlorophyll fluorescence parameters, leaf moisture status, biomass, 100-grain weight, hormone content, ear characteristics and grain yield of maize.     

猪脐静脉血管内皮细胞的分离及体外培养研究

为建立一种操作简单并且能获得大量猪血管内皮细胞的分离培养方法,本研究采集新生健康长白猪脐带,取其脐静脉血管,灌注0.1%的Ⅰ型胶原酶,于37℃水浴消化10 min后收集内皮细胞,培养于含20%胎牛血清的M199培养基中,长满单层后用胰酶消化传代培养。并对培养的细胞形态学、相关抗原和其他特征以及对病毒的易感性进行鉴定。结果表明,Ⅰ型胶原酶消化后可获得大量内皮细胞,细胞培养后的形态学观察显示细胞贴壁生长良好,呈典型的铺路石状排列;第Ⅷ因子相关抗原和细胞摄取乙酰化低密度脂蛋白均呈阳性,内皮细胞比率可高达100%。传3代后的生长曲线显示细胞传代后延迟期小于1 d,对数生长期约3 d,第5 d进入平台期,第7 d开始脱落死亡。病毒感染试验表明所获得的内皮细胞对猪瘟病毒高度易感,病毒生长与常用的PK-15没有区别。上述研究结果表明本研究建立的血管内皮细胞分离培养方法简便有效,为大量制备原代血管内皮细胞提供了可靠的方法。     

罗布麻属和白麻属种子形态特征及生活力的测定方法

以采自新疆维吾尔自治区阿勒泰市阿拉哈克镇境内的罗布麻属(Apocynum)和白麻属(Poacynum) 8个基因型植物的种子为材料,进行了8个基因型植物种子形态特征、千粒重和生活力测定方法探究。研究结果显示,参试罗布麻种子的长为2.75 mm,宽为0.68 mm,厚为0.49 mm。白麻属7个基因型中以紫斑中花的种子最长,为4.51 mm;青杆白花最宽,为0.90 mm;青杆白花和大叶白麻最厚,为0.61 mm。8个基因型种子的长、宽、高的变化范围分别为2.75～4.51、0.68～0.90和0.48～0.61 mm。其中,紫斑中花为最大基因型,罗布麻为最小基因型。千粒重变化范围在0.38～1.32 g,所有参试种子颜色均为褐色,其中以大叶白麻颜色最深。参试种子均表现出非休眠性种子萌发的特征,萌发时间较短,可以用来快速测定参试种子的生活力。采用四唑染色测定生活力时,参试种子均表现出不透四唑的特征,应该纵切切破种皮,预湿时间为12～14 h,染色时间为12 h,鉴定标准为种子胚80%完全染色。此次针对罗布麻属和白麻属种子形态特征与生活力测定方法的研究,能够为罗布麻种子生活力鉴定、大规模种...     

Molecular cloning and expression of kidney‐type glutaminase from common carp (Cyprinus carpio) and its up‐regulation by glutamine in primary culture enterocyte

Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln.