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B Costas P C N P Rêgo I Simões J F Marques M Castro‐Cunha A Afonso 《Journal of fish diseases》2013,36(6):543-553
The present study aimed to investigate leucocyte responses to inflammation as well as some innate immune parameters of Senegalese sole, Solea senegalensis, following challenge with two strains of Photobacterium damselae subsp. piscicida belonging to the European and Japanese clones described for this bacterium. Pathogenicity assays were performed to assess the virulence of each Photobacterium damselae subsp. piscicida strain for sole. Subsequently, fish were intraperitoneally injected with phosphate‐buffered saline (control) or two concentrations (2 × 102 and 2 × 106 CFU mL?1) of each bacterial strain and sampled after 6 and 24 h. Results showed that the European isolate induces a higher degree of response than the Japanese strain. While blood neutrophilia and monocytosis correlated well with the increase in neutrophil and macrophage numbers in the peritoneal cavity, fish infected with the European isolate presented higher peritoneal cell numbers than fish challenged with the Japanese strain. In addition, alternative complement pathway activity and respiratory burst of head kidney leucocytes increased significantly in fish infected with the European isolate. The enhanced innate immune response displayed by Senegalese sole challenged with the European isolate is probably due to the higher degree of virulence presented by this Photobacterium damselae subsp. piscicida strain. 相似文献
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Cândida Toni Alexssandro Geferson Becker Larissa Novaes Simões Carlos Garrido Pinheiro Lenise de Lima Silva Berta Maria Heinzmann Braulio Otomar Caron Bernardo Baldisserotto 《Fish physiology and biochemistry》2014,40(3):701-714
The anesthetic activities of the essential oils (EOs) of Hesperozygis ringens (EOHR) and Lippia alba (EOLA) and their effects in silver catfish (Rhamdia quelen) after anesthesia and recovery were investigated. Fish (32.19 ± 1.24 g) were submitted to one of the following treatments for each EO: basal group, control, or anesthesia (150, 300, or 450 μL L?1 EO). After that the anesthesia was induced or simulated and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. Fish were sampled at 0, 15, 30, 60, and 240 min after recovery. At time 0 of recovery, the ventilatory rate was lower in the groups anesthetized with either EO. In comparison with the basal group, control fish showed an increase in plasma glucose, aspartate aminotransferase (AST), and Na+ levels and a reduction in Na+/K+-ATPase activity at 0 min of recovery. Plasma levels of ammonia and Na+ were lower in the fish anesthetized with EOLA (450 μL L?1) and EOHR (all concentrations), respectively, than in the control fish. Additionally, lactate, AST, alanine aminotransferase, K+ plasma levels, and gill Na+/K+-ATPase and H+-ATPase activities were higher in the fish anesthetized with either EOHR or EOLA than in the control fish. The EOs promoted slight changes in silver catfish that enabled both an adaptive response and the recovery of most of the measured parameters after 240 min regardless of concentration or EO that was used. These findings support the use of EOHR and EOLA as anesthetics for fish. 相似文献
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Hyun-Joo Kim Mi Jung Kim Eun-Yeong Sim Choon Ki Lee Yong Hee Jeon Sun Lim Kim Gun-Ho Jung Beom-Young Son Koan Sik Woo 《Journal of Crop Science and Biotechnology》2018,21(1):57-65
We evaluated the proximate composition, free sugar content, fatty acid composition, carotenoid content, total phenol content, and radical scavenging activity of the grain from various Korean maize hybrid cultivars grown in two different cropping seasons. The moisture, crude ash, crude fat, crude protein, total starch, and amylose contents were significantly higher in most of the maize hybrid cultivars when grown in the early-season than when grown in the late-season. The free sugar content, fatty acid composition, carotenoid content, and total phenol content differed significantly between cultivars and cropping seasons. The highest unsaturated fatty acid compositions of maize hybrids of early-season and late-season were 86.05 and 86.29%, respectively, in the Daanok cultivar. The carotenoid contents were significantly higher in maize hybrids of late-season compared to those of early-season. The highest total phenol content was 108.09 mg/100 g in Singwangok of the late-season. The radical scavenging activity of maize hybrids differed significantly between cultivars and cropping seasons. 相似文献
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Camilo Allyson Simões de Farias Celso Augusto Guimarães Santos 《Journal of Soils and Sediments》2014,14(7):1242-1250
Purpose
We investigated the application of Kohonen Neural Networks (KNNs) in order to estimate sediment yield based on runoff and climatological data in a semiarid region of Brazil. Accurate estimations of sediment yield are essential to improve the management of soil erosion in semiarid areas, where large quantities of sediments tend to be produced only periodically.Materials and methods
The case study is an erosion plot within the São João do Cariri Experimental Basin, which is located in the semiarid portion of Paraíba State, Brazil. KNNs are unsupervised neural networks capable of reducing a multidimensional data set to a bidimensional matrix of features, which can be used for analysis and prediction purposes. A total of 60 rainfall events, which occurred between 1999 and 2002, were used to calibrate and test the model. The application of a multivariate linear regression (MLR) model was also carried out.Results and discussion
Statistical indexes were used as criteria for evaluating the performance of the KNN and MLR models for the test data set. The correlation and relative bias of the KNN model estimations with those from observed data were 0.90 and ?4.39 %, respectively. A correlation of 0.70 and a relative bias of 15.63 % were found from the comparison of sediment yields obtained by the MLR model with those of the observed data. Analysis of the outcomes indicates that the KNN model, which is capable of detecting and extracting nonlinear trends, produced more reliable results than the regression model.Conclusions
The KNN model results appear to be superior to those generated by the MLR model and suggest that the developed methodology may be applied to similar case studies. 相似文献49.
Galiza GJ Garcia HA Assis AC Oliveira DM Pimentel LA Dantas AF Simões SV Teixeira MM Riet-Correa F 《Veterinary parasitology》2011,182(2-4):359-363
Here, we report an outbreak of Trypanosoma vivax-induced trypanosomosis in Brazilian hair sheep on a farm in Paraíba state, a non-endemic region in northeastern Brazilian. Of 306 total sheep, 240 showed clinical signs and 216 died. Clinical signs included anorexia, lethargy, anemia, rough hair coat, weight loss, submandibular edema, abortion, and in some cases, neurological signs such as head pressing, lateral recumbence, paddling movements and muscle tremors. T. vivax was identified by blood smear analysis and polymerase chain reaction (PCR). At necropsy, animals exhibited watery blood, pale tissue coloring, and the presence of liquid in the peritoneal cavity and pericardial sac. Histologically, nonsuppurative myocarditis and meningoencephalitis with areas of malacia were observed. After treatment, no parasites were detected by blood smear analysis or PCR. Cattle and buffalo that remained in the same pasture were also infected but presented with asymptomatic infections. Epidemiological data suggest that T. vivax was introduced to the farm and the susceptible flock by buffalos that were asymptomatic carriers of the infection; T. vivax was most likely transmitted by Tabanus spp. bites and also iatrogenically. 相似文献
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Verónica González María Díez-Ortiz Mariano Simón Cornelis A. M. van Gestel 《Journal of Soils and Sediments》2011,11(7):1199-1208