广西玉米纹枯病菌培养性状及其致病力研究

从广西玉米主产区的8个县市秋玉米的纹枯病病株中分离出8个玉米纹枯病菌株,经生物性状的观察和菌丝融合群测定表明,它们都属于立枯丝核菌AGk-1-IA融合群;腐生定殖能力和致病力测定表明,菌株GX4、GX1的腐生定殖能力和致病力最强。     

无公害高山辣椒高效栽培技术

介绍了无公害高山辣椒高效栽培技术,主要包括：品种选择、播种育苗、合理定植、大田管理、病虫害防治和适时采收等,以期为发展山区经济、致富山区农民提供参考。     

Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits

OBJECTIVE: To investigate the effects of acupuncture on neural activity detected by use of manganese-enhanced functional magnetic resonance imaging (fMRI) and elucidate the relationship between somatic acupoint stimulation and brain activation. ANIMALS: 40 New Zealand White rabbits. PROCEDURE: Manganese-enhanced fMRI was performed in anesthetized rabbits manipulated with electroacupuncture (EA) on Zusanli (ST-36) and Yanglingquan (GB-34) acupoints. Image acquisition was performed on a 1.5T superconductive clinical scanner with a circular polarized extremity coil. T1-weighted images were acquired sequentially as follows: baseline, after mannitol injection, after manganese infusion, and 5 and 20 minutes after initiation of EA. RESULTS: Changes in focal neural activity were detected by use of manganese-enhanced fMRI. Stimulation on Zusanli (ST-36) for 5 minutes resulted in activation of the hippocampus, whereas stimulation on Yanglingquan (GB-34) resulted in activation of the hypothalamus, insula, and motor cortex. Activation became less specific after 20 minutes of EA. Furthermore, stimulation on ipsilateral acupoints led to bilateral brain activation. CONCLUSIONS AND CLINICAL RELEVANCE: Each acupoint has a corresponding cerebral linkage, and stimulation on these points resulted in time-dependent neural activation. Understanding the linkage between peripheral acupoint stimulation and central neural pathways may provide a useful guide for clinical applications of acupuncture.     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

胡萝卜黄色突变体的遗传及表现研究

 从胡萝卜‘新黑田五寸’品种的高世代自交系中发现了能稳定遗传的黄色突变体。遗传分析证明该黄色突变性状为单基因隐性遗传, 绿色对黄色为完全显性。黄色突变体叶片中叶绿素含量仅为正常株的29.3% ～50.1% , 且黄色不受温度光照等环境的影响。黄色突变体( yelyel) 地上部和地下部的生长量明显少于正常株系( YELYEL ) , 但在等位基因杂合时( YELyel) 与正常株系的生长量、产量和营养品质无显著差异。     

气候生态与栽培方式对砂梨生长结果习性的影响

在对地处同一纬度、不同海拔高度的利川市与荆州市的生态条件与砂梨栽培方式进行调查分析的基础上,探讨了气候生态条件和栽培方式对湖北省砂梨３个主栽品种黄花、湘南和长十郎的生长结果习性的影响。结果表明,鄂西南海拔１０００ｍ左右山地砂梨品种表现为干周大、树势强、物候期比江汉平原推迟１３～４０ｄ,相邻两物候期延长５～３１ｄ,树体生长期缩短２３～２５ｄ,新梢生长量、花序坐果率和花朵坐果率均极显著高于江汉平原,短果枝比例极显著低于江汉平原。两者对各品种之间的影响也存在不同程度的差异。     

不同含水量菜豆种子老化过程中生理特性的研究

 对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。     

牦牛源正呼肠孤病毒2型的检测和分离鉴定

旨在调查川西北牦牛哺乳动物正呼肠孤病毒(MRV)的感染情况并分离病毒.采用RT PCR方法,对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测,阳性样本进一步用分型PCR确定其血清型.结果 显示,粪便样本中MRV检出率为20.83％(15/72),血清2型的比例为60％(...     

草莓杯装软罐头的研制

试验对杯装草莓罐头的工艺进行了研究。结果表明,烫漂的工艺为75 ℃、4 min(分钟);注液汤汁的配比为白砂糖25 %、柠檬酸0.3 %、氯化钙0.2 %;杀菌工艺为7.5 kGy辐射杀菌。     

不同低温处理对桃1年生枝相对电导率的影响

以9个不同桃品种1年生枝条为试材。通过在-5℃,-10℃,-15℃,-20℃,-25℃,-30℃处理24h后,测定不同低温胁迫下桃枝条的相对电导率。结果表明:随着温度下降,相对电导率逐步上升,呈S型变化;不同桃种类间抗寒性表现为毛桃蟠桃油桃;不同熟期桃品种间抗寒性表现为晚熟早熟中熟;不同桃品种间依半致死温度其抗寒性表现为早露蟠桃金秋早金锦霞瑞蟠4号瑞蟠3号晚金大久保艳霞。