非洲鸵鸟的雌雄鉴别研究

对鸵鸟进行早期的性别鉴定，可以确保期望的鸵鸟雌雄性别比例，雏鸟雌雄分群饲养，加快鸵鸟驯养进程，提高育雏成活率都有着重要意义。本研究采用翻肛法，对人工饲养的1～3月龄育雏期非洲鸵鸟进行试验与研究。结果表明，在1月龄时就可根据有无生殖交媾器及其形态、大小的差别进行雌雄鉴别，打破了通常认为鸵鸟在4月龄时才可以进行性别鉴定的传统做法，为非洲鸵鸟的饲养管理提供了有利的依据。     

酸奶消费者购买行为的实证研究

以在校大学生问卷调查为例,应用联合分析法,运用极差分析、方差分析的统计学方法得出结论:价格是影响消费者酸奶购买行为的最显著因素;品牌和包装类型对酸奶购买的影响不相上下;净含量和保质期对消费者酸奶购买影响较小。乳品企业应针对不同的消费群体开发相应的产品、制定不同的市场营销策略。     

三峡库区农户肉羊肥育状况及肉用性能分析

三峡库区重庆境内沿江的区县(如移民大县云阳县等)山羊业发展一直处于全市前列,已逐渐成为农民稳定增收的重要产业。我们针对一些养殖大户对山羊杂交改良和饲养管理实践中存在的困惑,结合前期研究成果,将具有明显生长优势、有市场需求和群众愿意采用的优良杂交组合和自制补饲料     

非典型鸡新城疫的发病原因及其防制措施

目前,鸡新城疫仍是危害养禽业发展的一种严重疫病,尽管通过免疫预防接种与综合防制可以有效地控制新城疫,但在一些鸡场仍时有发生,大多以非典型鸡新城疫形式发病。     

大约克夏生长肥育期生长规律的研究

对60头大约克夏从70～190日龄,每隔15d测定体重和背膘厚,分析其生长发育规律。结果表明:①大约克夏生长肥育期的各阶段日龄与体重间的相关系数r≥0.67(P<0.0001)。②Logistic模型和Compertz模型均可拟合大约克夏在生长肥育期的生长曲线(P<0.0001),但Logistic模型(R2=0.9967)优于Compertz模型(R2=0.9694)。③大约克夏130￣160日龄间的背膘厚度与体重保持极小的比值(约0.11),160日龄前背膘厚的分化生长率小于1(0.2653￣0.7711);大约克夏190日龄时体重为119.31kg,背膘厚为14.59mm。     

Mechanisms for protection of berberine against LPS-induced acute lung injury in mice

AIM:To investigate the mechanisms by which berberine attenuates LPS-induced acute lung injury, and provide a new strategy for the treatment of the lung injury due to LPS. METHODS:BALB/c mice were randomly assigned into three groups (control, LPS group, and berberine treatment group). Mice were administered intragastrically with distilled water (0.1 mL/10 g) or neutral sulfate berberine (50 mg/kg) once a day for 3 days, 1 h after intragastrical treatment on day 3, LPS (20 mg/kg) or normal saline was injected intraperitoneally (ip). All animals were sacrificed 12 h after LPS injection, the left lung tissue sections were prepared for histology analysis and the right lung were used to determine the ratio of wet to dry lung tissue weight (W/D). In another experiment, bronchoalveolar lavage fluid (BALF) was collected, and then the total protein content, and the amounts of white blood cells (WBC) and polymorphonuclear neutrophils (PMN) in BALF were determined. Furthermore, the phosphorylation of cytosolic phospholipase A₂ (cPLA₂) was detected with immunohistochemical analysis by using phospho-cPLA2(Ser505) antibody, and the contents of thromboxane B₂ (TXB₂) in BALF, malondialdehyde (MDA) in the lungs, and activity of superoxide dismutase (SOD) in lung tissues were also determined.RESULTS:LPS induced acute lung injury, activated cPLA₂, and increased TXB₂ content in the BALF and MDA level in the lung tissue. The pretreatment with berberine significantly attenuated lung injury, lung edema and protein leakage induced by intraperitoneal injection of LPS. The expression of phospho-cPLA₂ in the lung tissues and TXB₂ content in the BALF in the berberine treatment group were lower than those in LPS group (P<0.05). In addition, the content of MDA in the lung tissue was lower in the berberine treatment group than LPS group (P<0.05), but there was no significant difference in activity of lung SOD between the berberine treatment and LPS group (P>0.05). CONCLUSION:Pretreatment with berberine remarkably reduces the LPS-induced lung injury, which is, at least in part, through inhibiting phosphorylation of cPLA₂ and decreasing lipid peroxidation. These findings provide a new strategy for the prevention and treatment of LPS-induced acute lung injury.     

低温胁迫对东方山羊豆幼苗生理特性的影响

以东方山羊豆及敖汉苜蓿为材料,研究低温胁迫对东方山羊豆和敖汉苜蓿可溶性蛋白含量、保护酶活性、渗透调节物质的影响.结果表明:在低温胁迫下,东方山羊豆及敖汉苜蓿脯氨酸含量、SOD、POD活性、可溶性蛋白含量先上升后下降.经对几项生理指标的综合分析,认为东方山羊豆比敖汉苜蓿具较强的抗低温能力.     

Effects of atorvastatin on cardiac myocyte hypertrophy of neonatal rat induced by angiotensinⅡ in vitro and TLR4 expression

AIM: To assess effects of atorvastatin (Ator) on cardiac myocytes (CM) hypertrophy of neonatal rat induced by angiotensinⅡ (AngⅡ) in vitro and Toll like receptor 4 (TLR4) expression for theoretical bases of preventing and treating myocardial hypertrophy.METHODS: CM of neonatal Sprague-Dawley (SD) rats were isolated with trypsin digestion method and those growth-arrested cells were stimulated with 10^-7 mol/L AngⅡ in the presence of various concentrations of Ator.The method of coomassie brilliant blue was adopted to evaluate the protein contents of CM.The changes in β-MHC,AT₁ receptor and TLR4 mRNA expression were observed by RT-PCR.RESULTS: ① AngⅡ increased the protein content of CM and β-MHC mRNA expression significantly,upregulated AT₁ receptor and TLR4 gene expression.② In a dose-dependent manner,Ator inhibited the increases in the protein contents of CM and β-MHC mRNA expression induced by AngⅡ.③ In a dose-dependent manner,Ator downregulated the AT₁ receptor and TLR4 mRNA expression of CM hypertrophy of neonatal rat induced by AngⅡ.CONCLUSION: Ator inhibits CM hypertrophy,downregulates the AT₁ receptor and TLR4 gene expression.     

Overexpressed human FAT10 protein induces the apoptosis in the starved HEK293 cells

AIM: To study the effect of human FAT10 on the apopotosis of HEK293 cells using flag-tagged human FAT10 protein.METHODS: The fragment of FAT10 gene was cloned into the pcDNA3-flag vector,which was identified by PCR,enzyme digestion and sequencing.The reconstructed plasmids were transfected into HEK293 cells.The expression of introduced FAT10 in the normal cultured and starved cells was detected respectively by Western blotting.XTT assay and DNA ladder method were used to analyze the effect of FAT10 on the apoptosis of starved HEK293 cells.RESULTS: The reconstructed plasmids were highly expressed in HEK293 cells with different expression mode at the mormal cultured and starved state.The livability of starved FAT10 overexpressed HEK293 cells was significantly lower than that of normal cultured cells.DNA ladder was observed in the starved FAT10 overexpressed cells,but not in the normal cultured cells.CONCLUSION: The eukaryotic expressed plasmids of flag-tagged FAT10 were constructed successfully,and highly expressed in HEK293.Overexpressed FAT10 enhances the apoptosis in the starved HEK293 cells.