血管回声跟踪技术对颈动脉脉搏波放大指数的检测及其意义

目的探讨血管回声跟踪技术(ET)对颈动脉脉搏波放大指数(AI)的检测及其临床意义。方法应用ET技术检测不同年龄正常人145例、高血压患者48例、糖尿病患者26例、终末期肾病20例的AI;正常健康人分为5个年龄组:20~29岁23人,30~39岁22人,40~49岁35人,50~59岁34人,≥60岁31人。比较不同性别及不同年龄组间的AI值有无差异;比较本组各年龄组ET检测的AI与其他方法测得同龄人的AI;比较上述各疾病与同龄正常人AI的差异。用SAS6.12做统计分析。结果AI无性别差异但有年龄差异:在上述5个年龄组的AI测值分别为5.2、7.6、12.8、16.2和20.8;各年龄组ET检测的AI与其他作者其他方法测定的AI相关性良好;高血压、糖尿病及终末期肾病患者的AI均较其同龄人增高。结论ET技术中的脉搏波放大指数AI能较好地反映颈动脉弹性情况,随着年龄增高,高血压、糖尿病及终末期肾病患者的AI明显增高。     

扁穗牛鞭草种质遗传多样性的ISSR分析

用ISSR标记对来自中国西南地区(四川、重庆、贵州)的28份扁穗牛鞭草材料的遗传多样性进行了检测。从96个ISSR引物中共筛选出13个多态性明显、反应稳定的引物。28份材料的DNA共扩增出129条谱带,平均每个引物扩增出9.9条带,多态性条带比率达84.2%。材料间遗传相似系数为0.466~0.980,表现出丰富的遗传多样性。通过聚类分析和主成分分析,将28份扁穗牛鞭草分为两大类,同一地区的扁穗牛鞭草品种(系)基本聚在同一类,呈现出一定的地域性分布规律。     

Purification and differentiation potentiality of fetal liver mesenchymal stem cells from mouse

AIM: To purify and investigate the differentiation potentials of fetal liver mesenchymal stem cells (flMSCs) from murine in vitro.METHODS: flMSCs from mouse fetuses at embryonic and fetal day (ED)13.5 or ED14.5 were isolated by adhering to plastic and passaged by modified method.Cell cycle and phenotype were analyzed by flow cytometry.The cell differentiation was induced by special induction media.The cells differentiated to adipose,cartilaginous and osteoid tissues were identified with oil red O,Toluid blue,alkaline phosphatease (ALP) and von Kossa’s staining.The cells differentiated to neural-like cells were detected by RT-PCR and immuno-staining.RESULTS: Fibroblast-like cells predominated in culture.(83.76±2.88)% of flMSCs stayed in the G₀/G₁ phases.Homogenous cells were positive for mesenchymal lineage markers CD44,CD29,but not for markers of hematopoietic cells CD45,CD11b.flMSCs were able to differentiate into adipogenic,chondrogenic,osteogenic and neurogenic cells.CONCLUSION: flMSCs can be purified by modified plastic-attachment method and have multiple differentiation,which is available to stem cell therapy for various diseases.     

不同退耕模式林草初夏小气候效应

通过对安塞县几种不同类型退耕还林还草模式,如混交林、人工灌木林、野生灌木林及林中革地进行了小气候观测,分析了不同退耕模式林草配置下的主要小气候特征,采用平行对比观测法定点定时观测了空气温湿度,土壤温度,水面蒸发并与对照裸地进行了对比.结果表明4种不同退耕模式林草配置在6月高温时期能显著降低空气温度和地表温度的日均值,提高空气湿度和土壤湿度,减少水面蒸发等,具有明显的调节功能.4种不同退耕模式日平均空气温度变化呈不对称的单峰曲线,较对照裸地可以降低0.41℃～1.2℃;0～20 cm各深度的土壤温度基本都是混交林＜人工灌木林＜野生灌木林＜林中草地＜对照裸地,混交林内地面最高温度为34.78℃,明显比对照裸地42.54℃低;空气相对湿度日动态呈"U"型变化,其中对照裸地相对温度日均值最低,混交林日均值最大,为55.2%,比对照裸地高3.95%;水面蒸发量为裸地最大,混交林及灌木林能有效减少水面蒸发,减少量较对照裸地可达50.54%和55.20%.     

东乡野生稻原产地蜘蛛群落结构动态研究

调查表明,东乡野生稻原产保护地蜘蛛群落优势科为狼蛛科、跳蛛科、管巢蛛科、球蛛科、肖蛸科,相对丰度分别为0.5010、0.1529、0.0785、0.0657、0.0559、0.0399、0.0392,其丰盛度总和达0.8540,构成了蜘蛛群落的主要成分;巨蟹蛛科为稀有科。优势种为拟水狼蛛、沟渠豹蛛、中华狼蛛、横窝希蛛、黄斑纯蛛、白纹舞蛛、微菱头蛛、柔弱锯螯蛛,其相对丰盛度分别为0.1802、0.1209、0.0833、0.0589、0.0484、0.0445、0.0417、0.0413,丰盛度总和达0.6192,埃比熊蛛等为稀有种。调查还表明,不同时期的物种丰富度变化不明显,但在6～7月间,群落中物种丰富度相对较高,各物种种群发生数量大,分布相对均匀,优势种不突出,群落结构较合理,均匀度和多样性指数较高。     

农药中毒的原因分析及防治对策

为探讨农业生产过程中农药中毒的发生原因及规律,我们在枣阳市的吴店、南城、兴隆和鹿头等乡镇进行了调查和试验,为广大农民科学掌握使用农药的方法进行了有益探索,并研究制定了科学有效的预防对策.     

稻水象甲幼虫发生程度对水稻生长发育及产量损失的影响

稻水象甲幼虫为害水稻根后,导致水稻植株矮缩、发黄、分蘖减少甚至死苗,造成水稻严重减产,本文对稻水象甲发生程度对水稻生长发育及产量损失的影响进行研究。     

湖北和安徽省油菜病毒病调查和病毒血清鉴定

2003年秋至2005年春2个油菜生长季分别到湖北和安徽2省12县市油菜产区进行病毒病发病情况调查,除局部地区和地块发病率达到20%～40%,多数大面积油菜发病率在0.1%以下。应用间接酶联免疫吸附法检测258份病害样品,TuMV占样品总数的90.7%,CMV占样品总数的8.9%,ORMV占样品总数的0.8%。     

Cloning of DNA fragments specific to the pathotype and race of <Emphasis Type="Italic">Verticillium dahliae</Emphasis>

Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction (PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race 1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB095266.