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41.
Precision Agriculture - Accurate and rapid diagnosis of nitrogen status in fruit trees on an individual tree basis is a prerequisite for precision orchard nutrient management. This study presents a... 相似文献
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Yamada O Kobayashi M Sugisaki O Ishii N Ito K Kuroki S Sasaki Y Isotani M Ono K Washizu T Bonkobara M 《Veterinary immunology and immunopathology》2011,142(1-2):101-106
Target therapy using the tyrosine kinase inhibitor imatinib is one of the new therapeutic approaches for canine mast cell tumors (MCTs). In the present report, we demonstrate a clinical response to imatinib in a dog with MCT carrying a c-kit c.1523A>T mutation. Moreover, the effect of this mutation on the phosphorylation status of KIT and the inhibitory potency of imatinib on the phosphorylation of the mutant KIT were examined in vitro. A dog with a MCT tumor mass on the right forelimb sole with lymph node metastasis and mastocytemia was treated with imatinib. The MCT mass markedly shrank and mastocytemia became undetectable with 2 weeks of treatment. The lymph node enlarged by metastasis became normal in size with 5 weeks of treatment. From the sequencing analysis of c-kit in tumor cells, a substitution mutation c.1523A>T that alters the amino acid composition (p.Asn508Ile) within the extracellular domain of KIT was identified. The mutant KIT expressed on 293 cells showed ligand-independent phosphorylation and imatinib suppressed this phosphorylation in a dose-dependent manner. From these findings, imatinib was considered to elicit a clinical response in a canine case of MCT via inhibition of the constitutively activated KIT caused by a c-kit c.1523A>T mutation. 相似文献
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Shiori Yabe Takashi Hara Mariko Ueno Hiroyuki Enoki Tatsuro Kimura Satoru Nishimura Yasuo Yasui Ryo Ohsawa Hiroyoshi Iwata 《Breeding Science》2014,64(4):291-299
For genetic studies and genomics-assisted breeding, particularly of minor crops, a genotyping system that does not require a priori genomic information is preferable. Here, we demonstrated the potential of a novel array-based genotyping system for the rapid construction of high-density linkage map and quantitative trait loci (QTL) mapping. By using the system, we successfully constructed an accurate, high-density linkage map for common buckwheat (Fagopyrum esculentum Moench); the map was composed of 756 loci and included 8,884 markers. The number of linkage groups converged to eight, which is the basic number of chromosomes in common buckwheat. The sizes of the linkage groups of the P1 and P2 maps were 773.8 and 800.4 cM, respectively. The average interval between adjacent loci was 2.13 cM. The linkage map constructed here will be useful for the analysis of other common buckwheat populations. We also performed QTL mapping for main stem length and detected four QTL. It took 37 days to process 178 samples from DNA extraction to genotyping, indicating the system enables genotyping of genome-wide markers for a few hundred buckwheat plants before the plants mature. The novel system will be useful for genomics-assisted breeding in minor crops without a priori genomic information. 相似文献
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Yuta ISHIZUKA Toru TAKEO Satohiro NAKAO Hidetaka YOSHIMOTO Yumiko HIROSE Yuki SAKAI Yuka HORIKOSHI Shiori TAKEUJI Shuuji TSUCHIYAMA Naomi NAKAGATA 《The Journal of reproduction and development》2014,60(6):454-459
Hyaluronidase is generally used to remove cumulus cells from mouse oocytes before oocyte cryopreservation, intracytoplasmic sperm injection or DNA injection. In general, use of cumulus-free mouse oocytes decreases in vitro fertilizing ability compared with cumulus-surrounded oocytes. The effect of hyaluronidase exposure on the quality of mouse oocytes is not fully understood. Here, we investigated the effect of hyaluronidase exposure time on the fertilization rate of fresh and vitrified mouse oocytes and their subsequent developmental ability in vitro. We found that the fertilization rate decreased with hyaluronidase treatments. This reduction in the fertilization rate following treatment with hyaluronidase was fully reversed by removal of the zona pellucida. In addition, oocytes treated with hyaluronidase for 5 min or longer had a reduced capacity to develop to the morula and blastocyst stage. The survival, fertilization, and
developmental rates of vitrified-warmed oocytes were also reduced by longer exposure to hyaluronidase. In conclusion, these results suggest that prolonged exposure to hyaluronidase decreases the quality of mouse oocytes and shorter hyaluronidase treatment times may help achieve a stable and high fertilization rate in fresh and cryopreserved oocytes. 相似文献
46.
Shiori Arai Mary Beth Callan 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(11):1066-1068
This report describes the presentation of acute arterial thrombosis causing triparesis in a 6-year-old female Chihuahua with pyometra and its successful management in combination with warfarin therapy. This is the first case report of a dog with arterial thrombosis associated with pyometra. 相似文献
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Masaki Michishita Shiori Ezaki Kikumi Ogihara Yuko Naya Daigo Azakami Takayuki Nakagawa Nobuo Sasaki Toshiro Arai Takuo Shida Kimimasa Takahashi 《Research in veterinary science》2014
Tumor-initiating cells (TICs) or cancer stem cells (CSCs), a small subset of tumor cells, are involved in tumor initiation, progression, recurrence and metastasis. In human hepatocellular carcinoma (HCC), TICs are enriched with cell surface markers and have the ability to self-renew and differentiate tumors at a high frequency. We established a canine HCC cell line, HCC930599, and analyzed it for stem and progenitor cell marker expression using flow cytometry. HCC930599 showed high CD44 and CD29, moderate CD90, and low CD133, CD34, CD24, CD117, and CD13 expression. CD90+CD44+ and CD90−CD44+ cells were characterized using the in vitro sphere assay and an in vivo transplant model. CD90+CD44+ cells acquired enhanced self-renewal capacity, proliferative activity and tumourigenicity compared with CD90−CD44+ cells, suggesting that TICs exist in the HCC930599 cell line and that CD90 is a marker for enriched TICs. Understanding TIC characteristics may help elucidate hepatic carcinogenesis and HCC therapy development. 相似文献
48.
Kanata Ibi Shiori Chiba Naomi Koyama Kazuto Hashimoto Hiroaki Nejishima 《Journal of toxicologic pathology》2021,34(2):147
Extraskeletal osteosarcoma is extremely rare in humans and animals, especially in rodents. This is the first case report on spontaneous extraskeletal osteosarcoma in the neck skeletal muscle of a Crlj:CD1 (ICR) mouse (36 weeks, dead). Necropsy revealed a solid white mass located in the neck skeletal muscle (scalenus muscle). Histological examination showed that the tumor consisted of atypical polygonal cells, a small osteoid clump, and bone tissue. Mitotic figures were observed. Serial sections showed that neoplastic cells lacked clear invasive proliferation to adjacent normal skeletal muscle and continuity with normal bone tissue. Immunohistochemical analysis showed that the neoplastic cells were positive for osteocalcin, osterix, vimentin, and S-100. Based on these results, the tumor was diagnosed as extraskeletal osteosarcoma in the neck skeletal muscle. 相似文献
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Akiyama T Maeda S Yamane S Ogino K Kasai M Kajiura F Matsumoto M Inoue J 《Science (New York, N.Y.)》2005,308(5719):248-251
The microenvironments of the thymus are generated by thymic epithelial cells (TECs) and are essential for inducing immune self-tolerance or developing T cells. However, the molecular mechanisms that underlie the differentiation of TECs and thymic compartmentalization are not fully understood. Here we show that deficiency in the tumor necrosis factor receptor-associated factor (TRAF) 6 results in disorganized distribution of medullary TECs (mTECs) and the absence of mature mTECs. Engraftment of thymic stroma of TRAF6(-/-) embryos into athymic nude mice induced autoimmunity. Thus, TRAF6 directs the development of thymic stroma and represents a critical point of regulation for self-tolerance and autoimmunity. 相似文献
50.
Shoshi Mizuta Yuuki Koizumi Shiori Inoue Chiaki Someya Masatomi Hosoi Yoshihiro Yokoyama Reiji Yoshinaka 《Fisheries Science》2013,79(5):833-839
A large size (400 kDa) non-collagenous protein was detected as a major component in the extract, with neutral salt solution, from the dermis of sea cucumber Apostichopus armata. On SDS-PAGE analysis, the 400 K component shifted to a lower molecular weight component (about 200 K) by reduction with 2-mercaptoethanol, and they were both reactive for periodic acid-Schiff (PAS) reaction staining. From these results, this protein was suggested to be a glycoprotein consisting of disulfide-bonded two subunits with almost equal molecular weight (200 K). In addition to relatively high contents (>100/1,000 residues) of aspartic and glutamic acids, cysteine was also detected (6.1/1,000 residues) in amino acid analyses of this protein partially purified by anion-exchange column chromatography. These combined results suggest the structural similarity of the 400 K component to fibronectins from other vertebrate and invertebrate animals. 相似文献