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81.
Tsukamoto A Ohno K Irie M Ohmi A Maeda S Nakashima K Fukushima K Fujino Y Uchida K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):503-506
Endoscopic polypectomy and argon plasma coagulation (APC) were performed in a refractory case of inflammatory colorectal polyps in a 7-year-old male Miniature Dachshund. Colonoscopic examination revealed a large sessile polyp and multiple diffuse small polyps, localized to the descending colon and rectum. The case showed a poor therapeutic response to prednisolone and cyclosporine. Under anesthesia, piecemeal resections were performed by polypectomy. APC was carried out to cauterize the polyp remnants. After treatment, reduction of the lesions and the improvement in clinical signs were observed, without recurrence of lesions for at least 10 months. Endoscopic treatment by polypectomy and APC is suggested to be a therapeutic option for refractory cases of inflammatory colorectal polyps in dogs. 相似文献
82.
K Nakamura M Yamasaki T Osaki H Ohta N Sasaki K Aoshima T Kimura M Takiguchi 《Journal of the American Animal Hospital Association》2012,48(5):327-330
Bilateral segmental aplasia of the uterine horns with unilateral pyometra and uterine horn torsion were diagnosed in a Pomeranian bitch that presented with chronic abdominal distension and an acute onset of anorexia and lethargy. Because radiographic and ultrasonographic findings revealed the presence of markedly enlarged bilateral uterine horns filled with fluid in the caudal abdomen, a tentative diagnosis of either pyometra or hydrometra with uterine horn torsion was made. Exploratory laparotomy showed bilateral, segmentally distended uterine horns with unilateral uterine horn torsion. Ovariohysterectomy was performed, and bilateral segmental aplasia of the uterine horns with the development of unilateral uterine horn torsion was diagnosed histopathologically. To the authors' knowledge, this is the first report of uterine horn torsion in conjunction with segmental aplasia of the uterine horn in a bitch. 相似文献
83.
To reveal the relationship between tolerance to submergence and the distribution ranges of riparian tree species in the floodplains
of the warm-temperate regions in Japan, we assessed the response and tolerance of current-year seedlings of two Salix and three Ulmaceae species to submergence treatments for 1, 2, 4, and 8 weeks. We investigated shoot elongation, number of
leaves, and survival rate of seedlings during the experiment, and biomass allocation was determined at the end of the experiment.
Shoot elongation and leaf production of seedlings were suppressed in all the species during the submergence treatments. However,
the seedlings of Salix chaenomeloides and S. pierotii elongated shoots rapidly and produced leaves after the treatments. All the underground biomass in these two species was larger
than aboveground biomass. These results are considered as typical responses of flood-tolerant species. In contrast, the underground
biomass of Ulmaceae seedlings (Celtis sinensis var. japonica, Aphananthe aspera, and Ulmus parvifolia) decreased more rapidly than aboveground biomass during prolonged submergence. Furthermore, A. aspera seedlings also showed suppression of shoot elongation and leaf production after the treatments. Our results indicate that
the three Ulmaceae species are less tolerant to submergence than the two Salix species. In conclusion, we suggest that submergence stress is one of the important factors determining the distribution of
tree species in the floodplains of lowlands in the warm-temperate regions of Japan. 相似文献
84.
Guanghua Wang Jun Murase Susumu Asakawa Makoto Kimura 《Biology and Fertility of Soils》2010,46(2):93-102
In order to evaluate the genetic diversity of cyanophage communities of rice fields, viral capsid assembly protein gene (g20) was amplified with primers CPS1 and CPS8. The DNA was extracted three times from viral concentrates obtained from floodwater
samples collected in each of four different plots (no fertilizer; P and K chemical fertilizers; N, P, and K chemical fertilizers;
and chemical fertilizers with compost). Denaturing gradient gel electrophoresis (DGGE) gave different g20 clones. The sequencing of DGGE bands revealed that the g20 genes of the floodwater were divergent and that the majority of clones formed several unique groups. However, they were more
closely related to g20 sequences from freshwaters than to those from marine waters, suggesting that g20 genes in terrestrial aquatic environments are different from those in marine environments. 相似文献
85.
Khaled Mohamed El-Dakhly El-Shaymaa El-Nahass Kosei Inui Junpei Kimura Hiroki Sakai Tokuma Yanai 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):421-424
An adult male rufous turtle dove, Streptopelia (S.) orientalis (Aves: Columbiformes), was found dead in Yorii-machi Town, Osato District 369-1217, Saitama Prefecture, Japan, and subjected to necropsy. A large number of immobile hypopi (deutonymphs) of the hypoderatid mite, Hypodectes (H.) propus (Acarina: Hypoderatidae), were found individually encapsulated subcutaneously primarily in the adipose tissue. The mites were 1.43 mm in length and 0.44 mm in width on average, and had provoked mild inflammatory reactions that predominantly manifested as foamy macrophages and lymphoplasmocytes. PCR analysis using ribosomal DNA extracted from paraffin-blocked tissues produced a 240 bp band specific for hypoderatids. Based on the morphological features (distinct coxal apodemes, especially in the anterior portion) and PCR-based findings, the hypopi were identified as H. propus. To the best of our knowledge, this is the first case describing the subcutaneous mite H. propus in a rufous turtle dove, S. orientalis, in Japan. This study also highlights the use of paraffin blocks as a source of tissue DNA for molecular evaluation. 相似文献
86.
Katsuhiko Yoshizawa Yuichi Kinoshita Yuko Emoto Ayako Kimura Norihisa Uehara Takashi Yuri Nobuaki Shikata Airo Tsubura 《Journal of toxicologic pathology》2013,26(2):141-148
N-Methyl-N-nitrosourea (MNU)-induced renal tumors in rats and Wilms tumors in humans were compared. Renal mesenchymal tumors (RMTs) and nephroblastomas (blastemal and epithelial components) in female Lewis rats treated with a single intraperitoneal injection of 50 mg/kg MNU at birth and Wilms tumors (blastemal, epithelial and mesenchymal components) in humans were analyzed for the expression of pancytokeratin (CK), vimentin, p63, α-smooth muscle actin (SMA), desmin, S-100, CD57, CD117/c-kit, Wilms tumor 1 protein (WT1) and β-catenin. The mesenchymal components of rat RMTs and human Wilms tumors expressed vimentin, SMA and β-catenin. The blastemal components of rat nephroblastomas and human Wilms tumors expressed vimentin, CD117/c-kit and β-catenin. The epithelial components of rat nephroblastomas and human Wilms tumors expressed vimentin and β-catenin. WT1 was expressed in different cellular components of rat tumors as compared with human Wilms tumors; the expression was seen in mesenchymal tumors and blastemal components of nephroblastomas in rats and epithelial components in human Wilms tumors. CK, p63 and CD57 were not expressed in rat RMTs or nephroblastomas, while CK and WT1 were expressed in epithelial components and CD57 was expressed in blastemal and epithelial components of human Wilms tumors. Rat and human tumors were universally negative for the expression of desmin and S-100. The immunohistochemical characteristics of rat renal tumors and human Wilms tumors may provide valuable information on the differences in renal oncogenesis and biology between the two species. 相似文献
87.
K Kaneko E Uematsu Y Takahashi B Tong S Takino Y Wajiki T Kimura H Yamashiro Y Kaneko H Iwaisaki T Sugiyama T Yamada S Yamagishi 《Reproduction in domestic animals》2013,48(6):1001-1005
This study aimed to develop a polymerase chain reaction (PCR)‐based sexing and effective semen collection methods for black‐headed and straw‐necked ibis species. However, most birds are not sexually dimorphic, that is, the sexes appear similar. Therefore, the gender should be determined before semen collection. DNA was extracted from the blood samples of 11 black‐headed and 4 straw‐necked ibis. The sex was determined after PCR amplification of the EE0.6 region of W‐chromosome. The PCR products were separated using gel electrophoresis. A single band indicated the presence of the EE0.6 region and that the individual was a female, while no band indicated that the individual was a male. Further, the single bands from seven specimens were amplified. Semen collection was performed by massage or a combination of massage with electro‐ejaculation and was attempted during all four seasons. The semen was successfully collected in March from male straw‐necked ibis using the massage method. Limited motility, viability and concentration of straw‐necked ibis sperm were observed. The sperm length was 180 μm and that of the nucleus was 30 μm with acrosome located at the tip of the nucleus. Thus, the PCR‐based sexing proved to be an accurate molecular sexing method for black‐headed and straw‐necked ibis. Furthermore, we successfully collected semen and observed the stained sperm nucleus and acrosome of the straw‐necked ibis sperm. We propose that the use of this PCR methodology can be applied as a routine method for sex determination and semen collection in ibis species for future ecological research. However, considering our limited success, further studies on semen collection method are required. 相似文献
88.
Mark K. Asplen Gianfranco Anfora Antonio Biondi Deuk-Soo Choi Dong Chu Kent M. Daane Patricia Gibert Andrew P. Gutierrez Kim A. Hoelmer William D. Hutchison Rufus Isaacs Zhi-Lin Jiang Zsolt Kárpáti Masahito T. Kimura Marta Pascual Christopher R. Philips Christophe Plantamp Luigi Ponti Gábor Vétek Heidrun Vogt Vaughn M. Walton Yi Yu Lucia Zappalà Nicolas Desneux 《Journal of pest science》2015,88(3):469-494
89.
Masashi?Hatamoto Takanori?Tanahashi Jun?Murase Kazuo?Matsuya Motoki?Hayashi Makoto?Kimura Susumu?AsakawaEmail author 《Biology and Fertility of Soils》2008,44(3):527-532
To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of
rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis
(DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities
in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed
of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which
existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were
associated with the decomposition of rice straw compost in paddy field soil directly or indirectly. 相似文献
90.
Hypocretin/orexin is produced exclusively in the dorsal and lateral hypothalamus but its projection is widespread within the brain and plays important roles. In this paper, we review the independent discoveries of the hypocretin/orexin peptides, the neuroanatomy of this system, and the link to the sleep disorder narcolepsy that has led to the idea that this system plays a crucial role in the regulation of sleep and wakefulness. 相似文献